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Increased levels of cell-free hemoglobin, oxidation markers, and the antioxidative heme scavenger alpha(1)-microglobulin in preeclampsia.

Gram, Magnus LU ; Centlow, Magnus LU ; Rutardottir, Sigurbjörg LU ; Stenfors, Iréne LU ; Larsson, Jörgen G LU ; Hosseini Maaf, Bahram LU ; Olsson, Martin L LU ; Hansson, Stefan LU and Åkerström, Bo LU (2010) In Free Radical Biology & Medicine 48. p.284-291
Abstract
Preeclampsia is a major cause of morbidity and mortality during pregnancy. To date, the pathogenesis of the disease is not fully understood. Recent studies show that preeclampsia is associated with overexpression of the hemoglobin genes alpha2 and gamma and accumulation of the protein in the vascular lumen of the placenta. Hypothesizing that cell-free hemoglobin leaks from the placenta into the maternal circulation and contributes to the endothelial damage and symptoms by inducing oxidative stress, we analyzed fetal and adult hemoglobin (HbF, HbA), haptoglobin, oxidation markers, and the heme scavenger and antioxidant alpha(1)-microglobulin in plasma, urine, and placenta in preeclamptic women (n=28) and women with normal pregnancy (n=27).... (More)
Preeclampsia is a major cause of morbidity and mortality during pregnancy. To date, the pathogenesis of the disease is not fully understood. Recent studies show that preeclampsia is associated with overexpression of the hemoglobin genes alpha2 and gamma and accumulation of the protein in the vascular lumen of the placenta. Hypothesizing that cell-free hemoglobin leaks from the placenta into the maternal circulation and contributes to the endothelial damage and symptoms by inducing oxidative stress, we analyzed fetal and adult hemoglobin (HbF, HbA), haptoglobin, oxidation markers, and the heme scavenger and antioxidant alpha(1)-microglobulin in plasma, urine, and placenta in preeclamptic women (n=28) and women with normal pregnancy (n=27). The mean plasma concentrations of HbF, HbA, protein carbonyl groups, membrane peroxidation capacity, and alpha(1)-microglobulin were significantly increased in preeclamptic women. The levels of total plasma Hb correlated strongly with the systolic blood pressure. The plasma haptoglobin concentrations of women with preeclampsia were significantly depressed. Increased amounts of alpha(1)-microglobulin mRNA and protein were found in placenta from preeclamptic women, and the levels of plasma and placenta alpha(1)-microglobulin correlated with the plasma Hb concentrations. The heme-degrading form t-alpha(1)-microglobulin was significantly increased in urine in preeclampsia. These results support the idea that hemoglobin-induced oxidative stress is a pathogenic factor in preeclampsia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Free Radical Biology & Medicine
volume
48
pages
284 - 291
publisher
Elsevier
external identifiers
  • wos:000273883800012
  • pmid:19879940
  • scopus:72649104840
ISSN
0891-5849
DOI
10.1016/j.freeradbiomed.2009.10.052
language
English
LU publication?
yes
id
1305abaf-3a5f-4a33-a97c-8eacd8c55a1e (old id 1512388)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19879940?dopt=Abstract
date added to LUP
2009-12-01 11:18:55
date last changed
2018-06-24 04:46:23
@article{1305abaf-3a5f-4a33-a97c-8eacd8c55a1e,
  abstract     = {Preeclampsia is a major cause of morbidity and mortality during pregnancy. To date, the pathogenesis of the disease is not fully understood. Recent studies show that preeclampsia is associated with overexpression of the hemoglobin genes alpha2 and gamma and accumulation of the protein in the vascular lumen of the placenta. Hypothesizing that cell-free hemoglobin leaks from the placenta into the maternal circulation and contributes to the endothelial damage and symptoms by inducing oxidative stress, we analyzed fetal and adult hemoglobin (HbF, HbA), haptoglobin, oxidation markers, and the heme scavenger and antioxidant alpha(1)-microglobulin in plasma, urine, and placenta in preeclamptic women (n=28) and women with normal pregnancy (n=27). The mean plasma concentrations of HbF, HbA, protein carbonyl groups, membrane peroxidation capacity, and alpha(1)-microglobulin were significantly increased in preeclamptic women. The levels of total plasma Hb correlated strongly with the systolic blood pressure. The plasma haptoglobin concentrations of women with preeclampsia were significantly depressed. Increased amounts of alpha(1)-microglobulin mRNA and protein were found in placenta from preeclamptic women, and the levels of plasma and placenta alpha(1)-microglobulin correlated with the plasma Hb concentrations. The heme-degrading form t-alpha(1)-microglobulin was significantly increased in urine in preeclampsia. These results support the idea that hemoglobin-induced oxidative stress is a pathogenic factor in preeclampsia.},
  author       = {Gram, Magnus and Centlow, Magnus and Rutardottir, Sigurbjörg and Stenfors, Iréne and Larsson, Jörgen G and Hosseini Maaf, Bahram and Olsson, Martin L and Hansson, Stefan and Åkerström, Bo},
  issn         = {0891-5849},
  language     = {eng},
  pages        = {284--291},
  publisher    = {Elsevier},
  series       = {Free Radical Biology & Medicine},
  title        = {Increased levels of cell-free hemoglobin, oxidation markers, and the antioxidative heme scavenger alpha(1)-microglobulin in preeclampsia.},
  url          = {http://dx.doi.org/10.1016/j.freeradbiomed.2009.10.052},
  volume       = {48},
  year         = {2010},
}