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Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium

Görgens, Johann F; van Zyl, WH; Knoetze, JH and Hahn-Hägerdal, Bärbel LU (2005) In Applied Microbiology and Biotechnology 67(5). p.684-691
Abstract
Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by... (More)
Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
volume
67
issue
5
pages
684 - 691
publisher
Springer
external identifiers
  • wos:000229722400014
  • pmid:15630584
  • scopus:21244466810
ISSN
1432-0614
DOI
10.1007/s00253-004-1803-3
language
English
LU publication?
yes
id
7c56cb4b-be29-4ca8-bfce-4a95a89f95f2 (old id 151244)
date added to LUP
2007-06-28 11:53:01
date last changed
2017-07-23 04:45:44
@article{7c56cb4b-be29-4ca8-bfce-4a95a89f95f2,
  abstract     = {Supplementation of a chemically defined medium with amino acids or succinate to improve heterologous xylanase production by a prototrophic Saccharomyces cerevisiae transformant was investigated. The corresponding xylanase production during growth on ethanol in batch culture and in glucose-limited chemostat culture were quantified, as the native ADH2 promoter regulating xylanase expression was derepressed under these conditions. The addition of a balanced mixture of the preferred amino acids, Ala, Arg, Asn, Glu, Gln and Gly, improved both biomass and xylanase production, whereas several other individual amino acids inhibited biomass and/or xylanase production. Heterologous protein production by the recombinant yeast was also improved by supplementing the medium with succinate. The production of heterologous xylanase during growth on ethanol or glucose could thus be improved by supplementing metabolic precursors in the carbon- or nitrogen-metabolism.},
  author       = {Görgens, Johann F and van Zyl, WH and Knoetze, JH and Hahn-Hägerdal, Bärbel},
  issn         = {1432-0614},
  language     = {eng},
  number       = {5},
  pages        = {684--691},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Amino acid supplementation improves heterologous protein production by Saccharomyces cerevisiae in defined medium},
  url          = {http://dx.doi.org/10.1007/s00253-004-1803-3},
  volume       = {67},
  year         = {2005},
}