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Derivatization using dimethylamine for tandem mass spectrometric structure analysis of enzymatically and acidically depolymerized methyl cellulose

Momcilovic, Dane LU ; Schagerlöf, Herje LU ; Röme, Daniel LU ; Jornten-Karlsson, M; Karlsson, KE; Wittgren, B; Tjerneld, Folke LU ; Wahlund, Karl-Gustav LU and Brinkmalm, G (2005) In Analytical Chemistry 77(9). p.2948-2959
Abstract
Structure analysis of partially depolymerized methyl cellulose was performed by nanoelectrospray ionization tandem mass spectrometry (nano-ESI-MS/MS) and by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS). Dimethylamine (DMA) was used for the first time as a reducing end derivatization reagent for oligosaccharides. This is an attractive reagent since it could be easily removed from the reaction mixture. Most important it also introduces a basic functional group that increased the sensitivity in both MALDI and nano-ESI. Depolymerization was made in two ways: one by the cellulose selective endoglucanase 5A from Bacillus agaradhaerens (Ba Ce15A) and the other by trifluoroacetic acid. The DMA derivatives... (More)
Structure analysis of partially depolymerized methyl cellulose was performed by nanoelectrospray ionization tandem mass spectrometry (nano-ESI-MS/MS) and by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS). Dimethylamine (DMA) was used for the first time as a reducing end derivatization reagent for oligosaccharides. This is an attractive reagent since it could be easily removed from the reaction mixture. Most important it also introduces a basic functional group that increased the sensitivity in both MALDI and nano-ESI. Depolymerization was made in two ways: one by the cellulose selective endoglucanase 5A from Bacillus agaradhaerens (Ba Ce15A) and the other by trifluoroacetic acid. The DMA derivatives formed both protonated and sodiated molecules in nano-ESI and MALDI. Tandem MS of protonated molecules yielded predominantly Y fragments from which the distribution of the substituents in the oligomers could be measured. Fragments obtained in tandem MS of sodiated molecules provided information regarding the positions of the substituents within the anhydroglucose units (AGUs). It was found that Ba Ce15A could cleave glucosidic bonds also if the AGU on the reducing side of the bond was fully methylated. The combination of DMA derivatization and tandem MS was demonstrated as a tool for the characterization of endoglucanase selectivity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
77
issue
9
pages
2948 - 2959
publisher
The American Chemical Society
external identifiers
  • wos:000228877500047
  • scopus:18144395782
ISSN
1520-6882
DOI
10.1021/ac048194e
language
English
LU publication?
yes
id
d1ab995c-e21d-4f43-bf95-3d998076c447 (old id 151453)
date added to LUP
2007-06-27 12:47:01
date last changed
2017-07-23 04:53:42
@article{d1ab995c-e21d-4f43-bf95-3d998076c447,
  abstract     = {Structure analysis of partially depolymerized methyl cellulose was performed by nanoelectrospray ionization tandem mass spectrometry (nano-ESI-MS/MS) and by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS). Dimethylamine (DMA) was used for the first time as a reducing end derivatization reagent for oligosaccharides. This is an attractive reagent since it could be easily removed from the reaction mixture. Most important it also introduces a basic functional group that increased the sensitivity in both MALDI and nano-ESI. Depolymerization was made in two ways: one by the cellulose selective endoglucanase 5A from Bacillus agaradhaerens (Ba Ce15A) and the other by trifluoroacetic acid. The DMA derivatives formed both protonated and sodiated molecules in nano-ESI and MALDI. Tandem MS of protonated molecules yielded predominantly Y fragments from which the distribution of the substituents in the oligomers could be measured. Fragments obtained in tandem MS of sodiated molecules provided information regarding the positions of the substituents within the anhydroglucose units (AGUs). It was found that Ba Ce15A could cleave glucosidic bonds also if the AGU on the reducing side of the bond was fully methylated. The combination of DMA derivatization and tandem MS was demonstrated as a tool for the characterization of endoglucanase selectivity.},
  author       = {Momcilovic, Dane and Schagerlöf, Herje and Röme, Daniel and Jornten-Karlsson, M and Karlsson, KE and Wittgren, B and Tjerneld, Folke and Wahlund, Karl-Gustav and Brinkmalm, G},
  issn         = {1520-6882},
  language     = {eng},
  number       = {9},
  pages        = {2948--2959},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Derivatization using dimethylamine for tandem mass spectrometric structure analysis of enzymatically and acidically depolymerized methyl cellulose},
  url          = {http://dx.doi.org/10.1021/ac048194e},
  volume       = {77},
  year         = {2005},
}