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Single cell analysis of the common lymphoid progenitor compartment reveals functional and molecular heterogeneity.

Månsson, Robert LU ; Zandi, Sasan LU ; Welinder, Eva LU ; Tsapogas, Panagiotis LU ; Sakaguchi, Nobuo; Bryder, David LU and Sigvardsson, Mikael LU (2010) In Blood 115(13). p.2601-2609
Abstract
In order to investigate molecular events involved in the regulation of lymphoid lineage commitment, we crossed lambda5 reporter transgenic mice to Rag1-GFP knock in mice. This allowed us to sub-fractionate common lymphoid progenitors (CLPs) and pre-pro-B (Fraction A) cells into lambda5(-)Rag1(low), lambda5(-)Rag1(high) and lambda5(+)Rag1(high) cells. Clonal in vitro differentiation analysis demonstrated that Rag1(low) cells gave rise to B/T and NK cells. Rag1(high) cells displayed reduced NK-cell potential with preserved capacity to generate B- and T-lineage cells, while the lambda5(+) cells were B-lineage restricted. Ebf1 and Pax5 expression was largely confined to the Rag1(high) populations. These cells also expressed a higher level of... (More)
In order to investigate molecular events involved in the regulation of lymphoid lineage commitment, we crossed lambda5 reporter transgenic mice to Rag1-GFP knock in mice. This allowed us to sub-fractionate common lymphoid progenitors (CLPs) and pre-pro-B (Fraction A) cells into lambda5(-)Rag1(low), lambda5(-)Rag1(high) and lambda5(+)Rag1(high) cells. Clonal in vitro differentiation analysis demonstrated that Rag1(low) cells gave rise to B/T and NK cells. Rag1(high) cells displayed reduced NK-cell potential with preserved capacity to generate B- and T-lineage cells, while the lambda5(+) cells were B-lineage restricted. Ebf1 and Pax5 expression was largely confined to the Rag1(high) populations. These cells also expressed a higher level of the surface protein LY6D, providing an additional tool for the analysis of early lymphoid development. These data suggest that the classical CLP compartment comprises a mixture of cells with relatively restricted lineage potentials, thus opening new possibilities to investigate early hematopoiesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
115
issue
13
pages
2601 - 2609
publisher
American Society of Hematology
external identifiers
  • wos:000276201000009
  • pmid:19996414
  • scopus:77951002652
ISSN
1528-0020
DOI
10.1182/blood-2009-08-236398
language
English
LU publication?
yes
id
a2ebe174-5887-4dff-bee7-d04bba9e91c6 (old id 1523769)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19996414?dopt=Abstract
date added to LUP
2010-01-14 12:59:32
date last changed
2018-07-15 03:13:32
@article{a2ebe174-5887-4dff-bee7-d04bba9e91c6,
  abstract     = {In order to investigate molecular events involved in the regulation of lymphoid lineage commitment, we crossed lambda5 reporter transgenic mice to Rag1-GFP knock in mice. This allowed us to sub-fractionate common lymphoid progenitors (CLPs) and pre-pro-B (Fraction A) cells into lambda5(-)Rag1(low), lambda5(-)Rag1(high) and lambda5(+)Rag1(high) cells. Clonal in vitro differentiation analysis demonstrated that Rag1(low) cells gave rise to B/T and NK cells. Rag1(high) cells displayed reduced NK-cell potential with preserved capacity to generate B- and T-lineage cells, while the lambda5(+) cells were B-lineage restricted. Ebf1 and Pax5 expression was largely confined to the Rag1(high) populations. These cells also expressed a higher level of the surface protein LY6D, providing an additional tool for the analysis of early lymphoid development. These data suggest that the classical CLP compartment comprises a mixture of cells with relatively restricted lineage potentials, thus opening new possibilities to investigate early hematopoiesis.},
  author       = {Månsson, Robert and Zandi, Sasan and Welinder, Eva and Tsapogas, Panagiotis and Sakaguchi, Nobuo and Bryder, David and Sigvardsson, Mikael},
  issn         = {1528-0020},
  language     = {eng},
  number       = {13},
  pages        = {2601--2609},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {Single cell analysis of the common lymphoid progenitor compartment reveals functional and molecular heterogeneity.},
  url          = {http://dx.doi.org/10.1182/blood-2009-08-236398},
  volume       = {115},
  year         = {2010},
}