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Improved matrix-assisted laser desorption/ionisation sample preparation of a partially depolymerised cellulose derivative by continuous spray deposition and interfacing with size-exclusion chromatography

Momcilovic, Dane LU ; Wahlund, Karl-Gustav LU ; Wittgren, B and Brinkmalm, G (2005) In Rapid Communications in Mass Spectrometry 19(7). p.947-954
Abstract
Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils.... (More)
Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils. The suitability for determination of the molar mass distribution of methyl cellulose was investigated. Copyright (c) 2005 John Wiley C Sons, Ltd. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Rapid Communications in Mass Spectrometry
volume
19
issue
7
pages
947 - 954
publisher
John Wiley & Sons
external identifiers
  • pmid:15747325
  • wos:000227907600012
  • scopus:15944410672
ISSN
1097-0231
DOI
10.1002/rcm.1874
language
English
LU publication?
yes
id
d43bad9f-f83f-4d6a-9bfe-9effa92f3fc4 (old id 152667)
date added to LUP
2007-06-27 12:49:11
date last changed
2017-01-01 04:34:42
@article{d43bad9f-f83f-4d6a-9bfe-9effa92f3fc4,
  abstract     = {Continuous spray deposition (CSD) of aqueous solutions of partially depolymerised methyl cellulose was found to improve matrix-assisted laser desorption/ionisation (MALDI) sample preparation. One feature was that the sensitivity in MALDI time-of-flight mass spectrometry increased up to an order of magnitude compared with the standard sample preparation method. Another feature was that CSD provided targets for MALDI with homogeneously distributed analyte. This resulted in a more even signal intensity and a higher reproducibility than in the standard method. High-mass discrimination was more pronounced in CSD than in the standard method. Size-exclusion chromatography with aqueous eluent was coupled online to CSD onto matrix-precoated foils. The suitability for determination of the molar mass distribution of methyl cellulose was investigated. Copyright (c) 2005 John Wiley C Sons, Ltd.},
  author       = {Momcilovic, Dane and Wahlund, Karl-Gustav and Wittgren, B and Brinkmalm, G},
  issn         = {1097-0231},
  language     = {eng},
  number       = {7},
  pages        = {947--954},
  publisher    = {John Wiley & Sons},
  series       = {Rapid Communications in Mass Spectrometry},
  title        = {Improved matrix-assisted laser desorption/ionisation sample preparation of a partially depolymerised cellulose derivative by continuous spray deposition and interfacing with size-exclusion chromatography},
  url          = {http://dx.doi.org/10.1002/rcm.1874},
  volume       = {19},
  year         = {2005},
}