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Facile Pretreatment of Bacilius circulans beta-Galactosidase Increases the Yield of Galactosyl Oligosaccharides in Milk and Lactose Reaction Systems

Gosling, Aaron; Alftrén, Johan LU ; Stevens, Geoff W.; Barber, Andrew R.; Kentish, Sandra E. and Gras, Sally L. (2009) In Journal of Agricultural and Food Chemistry 57(24). p.11570-11574
Abstract
The commercially available preparation of beta-galactosidase from Bacillus circulans, known as Biolacta FN5, has been extensively used in the production of prebiotic galactooligosaccharides (GOS). This study focuses on characterizing the production of GOS in two reaction systems: 10% lactose (w/v) in buffer and skim milk. Analysis of the temperature dependence of the GOS yield along with the relative rates of GOS synthesis and degradation leads to the finding that GOS degradation activity was selectively decreased in Biolacta FN5 above 40 degrees C. Facile heat treatment of Biolacta FN5 solution prior to use allowed for GOS yields to be significantly increased in both reaction systems.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-galactosidase, galactosyl transfer, prebiotic, Galactooligosaccharides, GOS, lactose hydrolysis, Bacillus circulans
in
Journal of Agricultural and Food Chemistry
volume
57
issue
24
pages
11570 - 11574
publisher
The American Chemical Society
external identifiers
  • wos:000272645300017
  • scopus:73349130732
ISSN
0021-8561
DOI
10.1021/jf9018596
language
English
LU publication?
yes
id
db9bc879-7c86-4e4f-9ea4-e0dcf0adcff4 (old id 1532310)
date added to LUP
2010-01-29 11:42:53
date last changed
2017-12-10 03:49:03
@article{db9bc879-7c86-4e4f-9ea4-e0dcf0adcff4,
  abstract     = {The commercially available preparation of beta-galactosidase from Bacillus circulans, known as Biolacta FN5, has been extensively used in the production of prebiotic galactooligosaccharides (GOS). This study focuses on characterizing the production of GOS in two reaction systems: 10% lactose (w/v) in buffer and skim milk. Analysis of the temperature dependence of the GOS yield along with the relative rates of GOS synthesis and degradation leads to the finding that GOS degradation activity was selectively decreased in Biolacta FN5 above 40 degrees C. Facile heat treatment of Biolacta FN5 solution prior to use allowed for GOS yields to be significantly increased in both reaction systems.},
  author       = {Gosling, Aaron and Alftrén, Johan and Stevens, Geoff W. and Barber, Andrew R. and Kentish, Sandra E. and Gras, Sally L.},
  issn         = {0021-8561},
  keyword      = {beta-galactosidase,galactosyl transfer,prebiotic,Galactooligosaccharides,GOS,lactose hydrolysis,Bacillus circulans},
  language     = {eng},
  number       = {24},
  pages        = {11570--11574},
  publisher    = {The American Chemical Society},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Facile Pretreatment of Bacilius circulans beta-Galactosidase Increases the Yield of Galactosyl Oligosaccharides in Milk and Lactose Reaction Systems},
  url          = {http://dx.doi.org/10.1021/jf9018596},
  volume       = {57},
  year         = {2009},
}