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Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2

Janicke, Birgit LU ; Önning, Gunilla LU and Oredsson, S M (2005) In Journal of Agricultural and Food Chemistry 53(17). p.6658-6665
Abstract
Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length... (More)
Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
p-coumaric acid, Cell cycle, proliferation, cereals, ferulic acid
in
Journal of Agricultural and Food Chemistry
volume
53
issue
17
pages
6658 - 6665
publisher
The American Chemical Society
external identifiers
  • pmid:16104781
  • wos:000231459900015
  • scopus:24344464239
ISSN
0021-8561
DOI
10.1021/jf050489l
language
English
LU publication?
yes
id
b4b7ef2f-f837-4f3c-82d3-3176515208f6 (old id 153233)
date added to LUP
2007-07-16 09:47:28
date last changed
2017-08-13 03:31:32
@article{b4b7ef2f-f837-4f3c-82d3-3176515208f6,
  abstract     = {Ferulic acid (FA) and para-coumaric acid (p-CA) may mediate the protective effects of whole-grain cereals against colon cancer. Therefore, the effects of FA and p-CA on the metabolic activity, proliferation, cell cycle phase distribution, and kinetics of the colonic endothelial tumor cell line Caco-2 was studied. Both compounds at 1500 M decreased the number of cells to 43-75% of control after 2-3 days of treatment. Cell cycle phase distribution and cell cycle kinetics were determined by flow cytometric analysis after bromodeoxyuridine labeling. Each compound at 1500 M decreased the proportion of cells in the G1 phase and increased the proportion of cells in the S and G2 phases. Treatment with 1500 M FA significantly increased the length of the S phase, while p-CA did not. It was concluded that FA and p-CA inhibited cell proliferation by presumably affecting different cell cycle phases, and this warrants further investigations because this inhibition may be one explanation for the diet-related protection against cancer.},
  author       = {Janicke, Birgit and Önning, Gunilla and Oredsson, S M},
  issn         = {0021-8561},
  keyword      = {p-coumaric acid,Cell cycle,proliferation,cereals,ferulic acid},
  language     = {eng},
  number       = {17},
  pages        = {6658--6665},
  publisher    = {The American Chemical Society},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Differential Effects of Ferulic Acid and p-Coumaric Acid on S Phase Distribution and Length of S Phase in the Human Colonic Cell Line Caco-2},
  url          = {http://dx.doi.org/10.1021/jf050489l},
  volume       = {53},
  year         = {2005},
}