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Quantification of Membrane Proteins Using Nonspecific Protease Digestions

Bendz, Maria; Carlsson Möller, Mirja LU ; Arrigoni, Giorgio LU ; Wåhlander, Åsa LU ; Stella, Roberto; Cappadona, Salvatore LU ; Levander, Fredrik LU ; Hederstedt, Lars LU and James, Peter LU (2009) In Journal of Proteome Research 8(12). p.5666-5673
Abstract
We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
membrane proteins, N-terminal labeling, quantification, relative, proteinase K, Bacillus subtilis
in
Journal of Proteome Research
volume
8
issue
12
pages
5666 - 5673
publisher
The American Chemical Society
external identifiers
  • wos:000272339100026
  • scopus:71549132222
ISSN
1535-3893
DOI
10.1021/pr900741t
language
English
LU publication?
yes
id
b5889499-14d3-4b76-b0a7-097924af96a8 (old id 1533438)
date added to LUP
2010-01-28 10:59:25
date last changed
2017-01-01 05:19:09
@article{b5889499-14d3-4b76-b0a7-097924af96a8,
  abstract     = {We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.},
  author       = {Bendz, Maria and Carlsson Möller, Mirja and Arrigoni, Giorgio and Wåhlander, Åsa and Stella, Roberto and Cappadona, Salvatore and Levander, Fredrik and Hederstedt, Lars and James, Peter},
  issn         = {1535-3893},
  keyword      = {membrane proteins,N-terminal labeling,quantification,relative,proteinase K,Bacillus subtilis},
  language     = {eng},
  number       = {12},
  pages        = {5666--5673},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Quantification of Membrane Proteins Using Nonspecific Protease Digestions},
  url          = {http://dx.doi.org/10.1021/pr900741t},
  volume       = {8},
  year         = {2009},
}