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Epitope shift of proteinase-3 anti-neutrophil cytoplasmic antibodies in patients with small vessel vasculitis.

Selga, Daina LU ; Segelmark, Mårten LU ; Gunnarsson, Lena LU and Hellmark, Thomas LU (2010) In Clinical and Experimental Immunology 160. p.318-324
Abstract
Summary Anti-neutrophil cytoplasmic antibodies against proteinase 3 (PR3-ANCA) are used as diagnostic tools for patients with small vessel vasculitis (AASV). We have produced chimeric mouse/human PR3 molecules and investigate changes in reactivity over time and the possible relationship between epitope specificity and clinical course. Thirty-eight PR3-ANCA-positive patients diagnosed between 1990 and 2003 were followed until December 2005. Plasma was collected at each out-patient visit and older samples were retrieved retrospectively. Patients reacted with multiple epitopes at the time of diagnosis. At subsequent relapses 12 patients shifted reactivity, in 11 cases from epitopes located in the C-terminal towards epitopes in the N-terminal.... (More)
Summary Anti-neutrophil cytoplasmic antibodies against proteinase 3 (PR3-ANCA) are used as diagnostic tools for patients with small vessel vasculitis (AASV). We have produced chimeric mouse/human PR3 molecules and investigate changes in reactivity over time and the possible relationship between epitope specificity and clinical course. Thirty-eight PR3-ANCA-positive patients diagnosed between 1990 and 2003 were followed until December 2005. Plasma was collected at each out-patient visit and older samples were retrieved retrospectively. Patients reacted with multiple epitopes at the time of diagnosis. At subsequent relapses 12 patients shifted reactivity, in 11 cases from epitopes located in the C-terminal towards epitopes in the N-terminal. Patients with reactivity against N-terminal parts of PR3 at diagnosis had a significantly lower relapse rate, 30% compared to 78% in the group with predominantly C-terminal reactivity (P = 0.04). The reactivity pattern did not correlate to outcome measured as death, end-stage renal disease or vasculitis activity index score (VDI) at 5 years. Further research is necessary to conclude if this is a general phenomenon. (Less)
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Contribution to journal
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published
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in
Clinical and Experimental Immunology
volume
160
pages
318 - 324
publisher
British Society for Immunology
external identifiers
  • wos:000277403100003
  • pmid:20059480
  • scopus:77952196152
ISSN
0009-9104
DOI
10.1111/j.1365-2249.2009.04063.x
language
English
LU publication?
yes
id
415186d9-97ad-49bd-8494-d05bc81c9dac (old id 1541283)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20059480?dopt=Abstract
date added to LUP
2010-02-02 11:56:46
date last changed
2018-05-29 09:34:17
@article{415186d9-97ad-49bd-8494-d05bc81c9dac,
  abstract     = {Summary Anti-neutrophil cytoplasmic antibodies against proteinase 3 (PR3-ANCA) are used as diagnostic tools for patients with small vessel vasculitis (AASV). We have produced chimeric mouse/human PR3 molecules and investigate changes in reactivity over time and the possible relationship between epitope specificity and clinical course. Thirty-eight PR3-ANCA-positive patients diagnosed between 1990 and 2003 were followed until December 2005. Plasma was collected at each out-patient visit and older samples were retrieved retrospectively. Patients reacted with multiple epitopes at the time of diagnosis. At subsequent relapses 12 patients shifted reactivity, in 11 cases from epitopes located in the C-terminal towards epitopes in the N-terminal. Patients with reactivity against N-terminal parts of PR3 at diagnosis had a significantly lower relapse rate, 30% compared to 78% in the group with predominantly C-terminal reactivity (P = 0.04). The reactivity pattern did not correlate to outcome measured as death, end-stage renal disease or vasculitis activity index score (VDI) at 5 years. Further research is necessary to conclude if this is a general phenomenon.},
  author       = {Selga, Daina and Segelmark, Mårten and Gunnarsson, Lena and Hellmark, Thomas},
  issn         = {0009-9104},
  language     = {eng},
  pages        = {318--324},
  publisher    = {British Society for Immunology},
  series       = {Clinical and Experimental Immunology},
  title        = {Epitope shift of proteinase-3 anti-neutrophil cytoplasmic antibodies in patients with small vessel vasculitis.},
  url          = {http://dx.doi.org/10.1111/j.1365-2249.2009.04063.x},
  volume       = {160},
  year         = {2010},
}