Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine.
(2010) In Free Radical Biology & Medicine 48(9). p.1211-1217- Abstract
- Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed... (More)
- Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed in plasma by radioimmunoassay. Median concentration of 8-oxodG was 8.3 nmol/L (adjusted for specific gravity), range 1.2-43, corresponding to a median of 4.7 mug/g creatinine, range 1.8-32. 8-oxodG was positively associated with urinary Cd (ss=0.32, p<0.001), urinary As (ss=0.0007, p=0.001), fraction of the monomethylated arsenic metabolite (MMA) in urine (ss=0.0026, p=0.004) and plasma Ft (ss = 0.20, p<0.001). A joint effect was seen for U-Cd and U-As, but whether this effect was additive or multiplicative was difficult to discern. (Less)
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https://lup.lub.lu.se/record/1552661
- author
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Free radicals, Lead, Arsenic, 8-OHdG, Cadmium, Ferritin, Oxidative stress, Pregnancy
- in
- Free Radical Biology & Medicine
- volume
- 48
- issue
- 9
- pages
- 1211 - 1217
- publisher
- Elsevier
- external identifiers
-
- wos:000276593800011
- pmid:20153423
- scopus:77950338676
- pmid:20153423
- ISSN
- 0891-5849
- DOI
- 10.1016/j.freeradbiomed.2010.02.004
- language
- English
- LU publication?
- yes
- id
- aea2b5f5-36ac-4a2e-8564-975ad2d310a7 (old id 1552661)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20153423?dopt=Abstract
- date added to LUP
- 2016-04-01 10:05:42
- date last changed
- 2022-03-04 08:01:09
@article{aea2b5f5-36ac-4a2e-8564-975ad2d310a7, abstract = {{Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed in plasma by radioimmunoassay. Median concentration of 8-oxodG was 8.3 nmol/L (adjusted for specific gravity), range 1.2-43, corresponding to a median of 4.7 mug/g creatinine, range 1.8-32. 8-oxodG was positively associated with urinary Cd (ss=0.32, p<0.001), urinary As (ss=0.0007, p=0.001), fraction of the monomethylated arsenic metabolite (MMA) in urine (ss=0.0026, p=0.004) and plasma Ft (ss = 0.20, p<0.001). A joint effect was seen for U-Cd and U-As, but whether this effect was additive or multiplicative was difficult to discern.}}, author = {{Engström, Karin and Vahter, Marie and Åkerman, Gabriella and Lindh, Christian and Teichert, Friederike and Singh, Rajinder and Kippler, Maria and Nermell, Barbro and Raqib, Rubhana and Strömberg, Ulf and Broberg Palmgren, Karin}}, issn = {{0891-5849}}, keywords = {{Free radicals; Lead; Arsenic; 8-OHdG; Cadmium; Ferritin; Oxidative stress; Pregnancy}}, language = {{eng}}, number = {{9}}, pages = {{1211--1217}}, publisher = {{Elsevier}}, series = {{Free Radical Biology & Medicine}}, title = {{Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine.}}, url = {{https://lup.lub.lu.se/search/files/1555610/1579169.pdf}}, doi = {{10.1016/j.freeradbiomed.2010.02.004}}, volume = {{48}}, year = {{2010}}, }