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Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine.

Engström, Karin LU ; Vahter, Marie; Åkerman, Gabriella LU ; Lindh, Christian LU ; Teichert, Friederike; Singh, Rajinder; Kippler, Maria; Nermell, Barbro; Raqib, Rubhana and Strömberg, Ulf LU , et al. (2010) In Free Radical Biology & Medicine 48(9). p.1211-1217
Abstract
Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed... (More)
Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed in plasma by radioimmunoassay. Median concentration of 8-oxodG was 8.3 nmol/L (adjusted for specific gravity), range 1.2-43, corresponding to a median of 4.7 mug/g creatinine, range 1.8-32. 8-oxodG was positively associated with urinary Cd (ss=0.32, p<0.001), urinary As (ss=0.0007, p=0.001), fraction of the monomethylated arsenic metabolite (MMA) in urine (ss=0.0026, p=0.004) and plasma Ft (ss = 0.20, p<0.001). A joint effect was seen for U-Cd and U-As, but whether this effect was additive or multiplicative was difficult to discern. (Less)
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Contribution to journal
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published
subject
keywords
Free radicals, Lead, Arsenic, 8-OHdG, Cadmium, Ferritin, Oxidative stress, Pregnancy
in
Free Radical Biology & Medicine
volume
48
issue
9
pages
1211 - 1217
publisher
Elsevier
external identifiers
  • wos:000276593800011
  • pmid:20153423
  • scopus:77950338676
ISSN
0891-5849
DOI
10.1016/j.freeradbiomed.2010.02.004
language
English
LU publication?
yes
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aea2b5f5-36ac-4a2e-8564-975ad2d310a7 (old id 1552661)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20153423?dopt=Abstract
date added to LUP
2010-03-02 22:08:52
date last changed
2018-07-01 03:06:10
@article{aea2b5f5-36ac-4a2e-8564-975ad2d310a7,
  abstract     = {Exposure to arsenic (As), cadmium (Cd) and lead (Pb) may generate oxidative stress, which can be assessed by 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in urine, a sensitive marker of oxidatively damaged DNA. We have evaluated oxidative stress induced by mixed chronic exposure to As, Cd, Pb, as well as the influence of As metabolism and nutritional status, i.e. ferritin (Ft), selenium (Se), zinc (Zn), manganese (Mn) and body weight. 8-oxodG was measured in urine from 212 women in early pregnancy from Matlab, rural Bangladesh, using LC-MS/MS. Cd and Pb were analyzed in urine and erythrocytes, while Se, Mn and Zn were analyzed in erythrocytes, all by ICPMS. As and As metabolites were analyzed in urine by HPLC-ICPMS. Ferritin was analyzed in plasma by radioimmunoassay. Median concentration of 8-oxodG was 8.3 nmol/L (adjusted for specific gravity), range 1.2-43, corresponding to a median of 4.7 mug/g creatinine, range 1.8-32. 8-oxodG was positively associated with urinary Cd (ss=0.32, p&lt;0.001), urinary As (ss=0.0007, p=0.001), fraction of the monomethylated arsenic metabolite (MMA) in urine (ss=0.0026, p=0.004) and plasma Ft (ss = 0.20, p&lt;0.001). A joint effect was seen for U-Cd and U-As, but whether this effect was additive or multiplicative was difficult to discern.},
  author       = {Engström, Karin and Vahter, Marie and Åkerman, Gabriella and Lindh, Christian and Teichert, Friederike and Singh, Rajinder and Kippler, Maria and Nermell, Barbro and Raqib, Rubhana and Strömberg, Ulf and Broberg Palmgren, Karin},
  issn         = {0891-5849},
  keyword      = {Free radicals,Lead,Arsenic,8-OHdG,Cadmium,Ferritin,Oxidative stress,Pregnancy},
  language     = {eng},
  number       = {9},
  pages        = {1211--1217},
  publisher    = {Elsevier},
  series       = {Free Radical Biology & Medicine},
  title        = {Chronic exposure to cadmium and arsenic strongly influences concentrations of 8-oxo-7,8-dihydro-2'-deoxyguanosine in urine.},
  url          = {http://dx.doi.org/10.1016/j.freeradbiomed.2010.02.004},
  volume       = {48},
  year         = {2010},
}