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Effects of Carbon Dioxide on Growth of Proteolytic Clostridium botulinum, Its Ability To Produce Neurotoxin, and Its Transcriptome

Artin, Ingrid LU ; Mason, David R.; Pin, Carmen; Schelin, Jenny LU ; Peck, Michael W.; Holst, Elisabet LU ; Rådström, Peter LU and Carter, Andrew T. (2010) In Applied and Environmental Microbiology 76(4). p.1168-1172
Abstract
The antimicrobial gas carbon dioxide is frequently used in modified atmosphere packaging. In the present study, the effects of CO2 (10 to 70%, vol/vol) on gene expression (measured using quantitative reverse transcription-PCR and a whole-genome DNA microarray) and neurotoxin formation (measured using an enzyme-linked immunosorbent assay [ELISA]) by proteolytic Clostridium botulinum type A1 strain ATCC 3502 were studied during the growth cycle. Interestingly, in marked contrast to the situation with nonproteolytic C. botulinum types B and E, CO2 had little effect on any of these parameters. At all CO2 concentrations, relative expression of neurotoxin cluster genes peaked in the transition between exponential and stationary phases, with... (More)
The antimicrobial gas carbon dioxide is frequently used in modified atmosphere packaging. In the present study, the effects of CO2 (10 to 70%, vol/vol) on gene expression (measured using quantitative reverse transcription-PCR and a whole-genome DNA microarray) and neurotoxin formation (measured using an enzyme-linked immunosorbent assay [ELISA]) by proteolytic Clostridium botulinum type A1 strain ATCC 3502 were studied during the growth cycle. Interestingly, in marked contrast to the situation with nonproteolytic C. botulinum types B and E, CO2 had little effect on any of these parameters. At all CO2 concentrations, relative expression of neurotoxin cluster genes peaked in the transition between exponential and stationary phases, with evidence of a second rise in expression in late stationary phase. Microarray analysis enabled identification of coding sequences whose expression profiles matched those of the neurotoxin cluster. Further research is needed to determine whether these are connected to neurotoxin formation or are merely growth phase associated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
76
issue
4
pages
1168 - 1172
publisher
American Society for Microbiology
external identifiers
  • wos:000274328900024
  • scopus:76649125882
  • pmid:20038699
ISSN
0099-2240
DOI
10.1128/AEM.02247-09
language
English
LU publication?
yes
id
bfc39259-c1ce-486e-9e4d-9f681e66138f (old id 1571218)
date added to LUP
2010-03-16 14:19:48
date last changed
2018-05-29 11:46:38
@article{bfc39259-c1ce-486e-9e4d-9f681e66138f,
  abstract     = {The antimicrobial gas carbon dioxide is frequently used in modified atmosphere packaging. In the present study, the effects of CO2 (10 to 70%, vol/vol) on gene expression (measured using quantitative reverse transcription-PCR and a whole-genome DNA microarray) and neurotoxin formation (measured using an enzyme-linked immunosorbent assay [ELISA]) by proteolytic Clostridium botulinum type A1 strain ATCC 3502 were studied during the growth cycle. Interestingly, in marked contrast to the situation with nonproteolytic C. botulinum types B and E, CO2 had little effect on any of these parameters. At all CO2 concentrations, relative expression of neurotoxin cluster genes peaked in the transition between exponential and stationary phases, with evidence of a second rise in expression in late stationary phase. Microarray analysis enabled identification of coding sequences whose expression profiles matched those of the neurotoxin cluster. Further research is needed to determine whether these are connected to neurotoxin formation or are merely growth phase associated.},
  author       = {Artin, Ingrid and Mason, David R. and Pin, Carmen and Schelin, Jenny and Peck, Michael W. and Holst, Elisabet and Rådström, Peter and Carter, Andrew T.},
  issn         = {0099-2240},
  language     = {eng},
  number       = {4},
  pages        = {1168--1172},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Effects of Carbon Dioxide on Growth of Proteolytic Clostridium botulinum, Its Ability To Produce Neurotoxin, and Its Transcriptome},
  url          = {http://dx.doi.org/10.1128/AEM.02247-09},
  volume       = {76},
  year         = {2010},
}