Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

SANS study of the interactions among DNA, a cationic surfactant, and polystyrene latex particles

Cárdenas, Marité LU ; Dreiss, C A ; Nylander, Tommy LU ; Chan, C P ; Cosgrove, T and Lindman, Björn LU (2005) In Langmuir 21(8). p.3578-3583
Abstract
The compaction of DNA by a cationic surfactant both in the bulk and adsorbed on the surface of latex particles was followed for the first time by SANS. In the bulk, a decrease in the overall size of the DNA coil in the presence of the cationic surfactant was observed at a negative-to-positive charge ratio far below the phase separation region, at a negative-to-positive charge ratio of 18. Additionally, large surfactant aggregates seem to form within the DNA-surfactant complex. On the other hand, DNA adsorbs onto the surface of latex particles, forming a thick layer, as evidenced by the fitting of the SANS data to a core-shell form factor. Addition of a cationic surfactant to the DNA-coated latex particles at a negative-to-positive charge... (More)
The compaction of DNA by a cationic surfactant both in the bulk and adsorbed on the surface of latex particles was followed for the first time by SANS. In the bulk, a decrease in the overall size of the DNA coil in the presence of the cationic surfactant was observed at a negative-to-positive charge ratio far below the phase separation region, at a negative-to-positive charge ratio of 18. Additionally, large surfactant aggregates seem to form within the DNA-surfactant complex. On the other hand, DNA adsorbs onto the surface of latex particles, forming a thick layer, as evidenced by the fitting of the SANS data to a core-shell form factor. Addition of a cationic surfactant to the DNA-coated latex particles at a negative-to-positive charge ratio of 38 induces a slight decrease in the size of the particle layer, where the cationic surfactant is evenly distributed within the adsorbed layer. A further decrease of the negative-to-positive charge ratio to 18 induces a dramatic change in the SANS data that suggests significant compaction of the adsorbed layer and the formation of large surfactant aggregates, similar to those detected in the bulk. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
21
issue
8
pages
3578 - 3583
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000228218700054
  • pmid:15807604
  • scopus:17444394006
ISSN
0743-7463
DOI
10.1021/la047251w
language
English
LU publication?
yes
id
71ca64a4-8162-4b91-a7bc-257c9deb4475 (old id 157240)
date added to LUP
2016-04-01 12:08:29
date last changed
2022-01-26 23:25:12
@article{71ca64a4-8162-4b91-a7bc-257c9deb4475,
  abstract     = {{The compaction of DNA by a cationic surfactant both in the bulk and adsorbed on the surface of latex particles was followed for the first time by SANS. In the bulk, a decrease in the overall size of the DNA coil in the presence of the cationic surfactant was observed at a negative-to-positive charge ratio far below the phase separation region, at a negative-to-positive charge ratio of 18. Additionally, large surfactant aggregates seem to form within the DNA-surfactant complex. On the other hand, DNA adsorbs onto the surface of latex particles, forming a thick layer, as evidenced by the fitting of the SANS data to a core-shell form factor. Addition of a cationic surfactant to the DNA-coated latex particles at a negative-to-positive charge ratio of 38 induces a slight decrease in the size of the particle layer, where the cationic surfactant is evenly distributed within the adsorbed layer. A further decrease of the negative-to-positive charge ratio to 18 induces a dramatic change in the SANS data that suggests significant compaction of the adsorbed layer and the formation of large surfactant aggregates, similar to those detected in the bulk.}},
  author       = {{Cárdenas, Marité and Dreiss, C A and Nylander, Tommy and Chan, C P and Cosgrove, T and Lindman, Björn}},
  issn         = {{0743-7463}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{3578--3583}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Langmuir}},
  title        = {{SANS study of the interactions among DNA, a cationic surfactant, and polystyrene latex particles}},
  url          = {{http://dx.doi.org/10.1021/la047251w}},
  doi          = {{10.1021/la047251w}},
  volume       = {{21}},
  year         = {{2005}},
}