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SANS study of the interactions among DNA, a cationic surfactant, and polystyrene latex particles

Cárdenas, Marité LU ; Dreiss, C A; Nylander, Tommy LU ; Chan, C P; Cosgrove, T and Lindman, Björn LU (2005) In Langmuir 21(8). p.3578-3583
Abstract
The compaction of DNA by a cationic surfactant both in the bulk and adsorbed on the surface of latex particles was followed for the first time by SANS. In the bulk, a decrease in the overall size of the DNA coil in the presence of the cationic surfactant was observed at a negative-to-positive charge ratio far below the phase separation region, at a negative-to-positive charge ratio of 18. Additionally, large surfactant aggregates seem to form within the DNA-surfactant complex. On the other hand, DNA adsorbs onto the surface of latex particles, forming a thick layer, as evidenced by the fitting of the SANS data to a core-shell form factor. Addition of a cationic surfactant to the DNA-coated latex particles at a negative-to-positive charge... (More)
The compaction of DNA by a cationic surfactant both in the bulk and adsorbed on the surface of latex particles was followed for the first time by SANS. In the bulk, a decrease in the overall size of the DNA coil in the presence of the cationic surfactant was observed at a negative-to-positive charge ratio far below the phase separation region, at a negative-to-positive charge ratio of 18. Additionally, large surfactant aggregates seem to form within the DNA-surfactant complex. On the other hand, DNA adsorbs onto the surface of latex particles, forming a thick layer, as evidenced by the fitting of the SANS data to a core-shell form factor. Addition of a cationic surfactant to the DNA-coated latex particles at a negative-to-positive charge ratio of 38 induces a slight decrease in the size of the particle layer, where the cationic surfactant is evenly distributed within the adsorbed layer. A further decrease of the negative-to-positive charge ratio to 18 induces a dramatic change in the SANS data that suggests significant compaction of the adsorbed layer and the formation of large surfactant aggregates, similar to those detected in the bulk. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
21
issue
8
pages
3578 - 3583
publisher
The American Chemical Society
external identifiers
  • wos:000228218700054
  • pmid:15807604
  • scopus:17444394006
ISSN
0743-7463
DOI
10.1021/la047251w
language
English
LU publication?
yes
id
71ca64a4-8162-4b91-a7bc-257c9deb4475 (old id 157240)
date added to LUP
2007-07-11 08:35:12
date last changed
2017-04-23 03:35:57
@article{71ca64a4-8162-4b91-a7bc-257c9deb4475,
  abstract     = {The compaction of DNA by a cationic surfactant both in the bulk and adsorbed on the surface of latex particles was followed for the first time by SANS. In the bulk, a decrease in the overall size of the DNA coil in the presence of the cationic surfactant was observed at a negative-to-positive charge ratio far below the phase separation region, at a negative-to-positive charge ratio of 18. Additionally, large surfactant aggregates seem to form within the DNA-surfactant complex. On the other hand, DNA adsorbs onto the surface of latex particles, forming a thick layer, as evidenced by the fitting of the SANS data to a core-shell form factor. Addition of a cationic surfactant to the DNA-coated latex particles at a negative-to-positive charge ratio of 38 induces a slight decrease in the size of the particle layer, where the cationic surfactant is evenly distributed within the adsorbed layer. A further decrease of the negative-to-positive charge ratio to 18 induces a dramatic change in the SANS data that suggests significant compaction of the adsorbed layer and the formation of large surfactant aggregates, similar to those detected in the bulk.},
  author       = {Cárdenas, Marité and Dreiss, C A and Nylander, Tommy and Chan, C P and Cosgrove, T and Lindman, Björn},
  issn         = {0743-7463},
  language     = {eng},
  number       = {8},
  pages        = {3578--3583},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {SANS study of the interactions among DNA, a cationic surfactant, and polystyrene latex particles},
  url          = {http://dx.doi.org/10.1021/la047251w},
  volume       = {21},
  year         = {2005},
}