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Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry

Månsson, Viktor LU ; Gilsdorf, Janet R. ; Kahlmeter, Gunnar ; Kilian, Mogens ; Kroll, J. Simon ; Riesbeck, Kristian LU orcid and Resman, Fredrik LU (2018) In Emerging Infectious Diseases 24(3). p.443-452
Abstract

Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for... (More)

Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a–f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Emerging Infectious Diseases
volume
24
issue
3
pages
10 pages
publisher
Centers for Disease Control and Prevention (CDC)
external identifiers
  • scopus:85042311159
  • pmid:29460728
ISSN
1080-6040
DOI
10.3201/eid2403.170459
language
English
LU publication?
yes
id
1575105b-c95e-4770-89f2-6ed493c52c93
date added to LUP
2018-03-17 18:57:38
date last changed
2025-10-14 12:15:18
@article{1575105b-c95e-4770-89f2-6ed493c52c93,
  abstract     = {{<p>Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a–f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a–f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.</p>}},
  author       = {{Månsson, Viktor and Gilsdorf, Janet R. and Kahlmeter, Gunnar and Kilian, Mogens and Kroll, J. Simon and Riesbeck, Kristian and Resman, Fredrik}},
  issn         = {{1080-6040}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{3}},
  pages        = {{443--452}},
  publisher    = {{Centers for Disease Control and Prevention (CDC)}},
  series       = {{Emerging Infectious Diseases}},
  title        = {{Capsule typing of haemophilus influenzae by matrix-assisted laser Desorption/Ionization time-of-flight mass spectrometry}},
  url          = {{http://dx.doi.org/10.3201/eid2403.170459}},
  doi          = {{10.3201/eid2403.170459}},
  volume       = {{24}},
  year         = {{2018}},
}