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Binding of the complement inhibitor C4b-binding protein to Lyme disease borreliae

Pietikainen, Johanna; Meri, Taru; Blom, Anna LU and Meri, Seppo (2010) In Molecular Immunology 47(6). p.1299-1305
Abstract
The Lyme disease spirochetes, Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii. are tick-borne pathogens that can cause chronic disseminated infections. To survive in the human host borreliae need to evade the immune system. It is already well known that B. burgdorferi ss. and B. afzelii bind the complement (C) alternative pathway inhibitor factor H from serum using OspE and CRASP-1/Bba68 proteins to escape C attack. In the presence of natural antibodies and in chronic infections, when specific antibodies develop, borreliae have to protect themselves from antibody-induced classical pathway C attack. In this study we demonstrate binding of the classical pathway inhibitor, C4b-binding protein (C4bp), to three... (More)
The Lyme disease spirochetes, Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii. are tick-borne pathogens that can cause chronic disseminated infections. To survive in the human host borreliae need to evade the immune system. It is already well known that B. burgdorferi ss. and B. afzelii bind the complement (C) alternative pathway inhibitor factor H from serum using OspE and CRASP-1/Bba68 proteins to escape C attack. In the presence of natural antibodies and in chronic infections, when specific antibodies develop, borreliae have to protect themselves from antibody-induced classical pathway C attack. In this study we demonstrate binding of the classical pathway inhibitor, C4b-binding protein (C4bp), to three genospecies of B. burgdorferi sensu lato. Binding was strongest to B. garinii, which has been found to be the weakest factor H binder. The bacteria bound both purified I-125-labeled C4bp and C4bp from serum. Unlabeled C4bp competed for binding with I-125-C4bp, whereas BSA had no effect. Binding was salt-sensitive and inhibited by C4b and partially by heparin. C4bp maintained its cofactor activity for factor I in cleaving C4b when bound to the bacterial surface. Ligand blotting analysis of whole cell lysates and fractionated outer cell membranes of the bacteria suggested one major receptor of approximately 43 kDa (P43) for C4bp in B. garinii and B. burgdorferi sensu stricto. Binding of C4bp may thus allow Lyme disease borreliae to escape activation of the classical C pathway and allow chronic infections in humans even in the presence of specific antibodies. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Innate immunity, Infection, Immune evasion
in
Molecular Immunology
volume
47
issue
6
pages
1299 - 1305
publisher
Pergamon
external identifiers
  • wos:000276300600016
  • scopus:77149129877
ISSN
1872-9142
DOI
10.1016/j.molimm.2009.11.028
language
English
LU publication?
yes
id
bfcdecfd-4a46-4430-ace5-3bdc70215a65 (old id 1586968)
date added to LUP
2010-04-27 12:13:47
date last changed
2018-05-29 10:20:36
@article{bfcdecfd-4a46-4430-ace5-3bdc70215a65,
  abstract     = {The Lyme disease spirochetes, Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii. are tick-borne pathogens that can cause chronic disseminated infections. To survive in the human host borreliae need to evade the immune system. It is already well known that B. burgdorferi ss. and B. afzelii bind the complement (C) alternative pathway inhibitor factor H from serum using OspE and CRASP-1/Bba68 proteins to escape C attack. In the presence of natural antibodies and in chronic infections, when specific antibodies develop, borreliae have to protect themselves from antibody-induced classical pathway C attack. In this study we demonstrate binding of the classical pathway inhibitor, C4b-binding protein (C4bp), to three genospecies of B. burgdorferi sensu lato. Binding was strongest to B. garinii, which has been found to be the weakest factor H binder. The bacteria bound both purified I-125-labeled C4bp and C4bp from serum. Unlabeled C4bp competed for binding with I-125-C4bp, whereas BSA had no effect. Binding was salt-sensitive and inhibited by C4b and partially by heparin. C4bp maintained its cofactor activity for factor I in cleaving C4b when bound to the bacterial surface. Ligand blotting analysis of whole cell lysates and fractionated outer cell membranes of the bacteria suggested one major receptor of approximately 43 kDa (P43) for C4bp in B. garinii and B. burgdorferi sensu stricto. Binding of C4bp may thus allow Lyme disease borreliae to escape activation of the classical C pathway and allow chronic infections in humans even in the presence of specific antibodies. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Pietikainen, Johanna and Meri, Taru and Blom, Anna and Meri, Seppo},
  issn         = {1872-9142},
  keyword      = {Innate immunity,Infection,Immune evasion},
  language     = {eng},
  number       = {6},
  pages        = {1299--1305},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {Binding of the complement inhibitor C4b-binding protein to Lyme disease borreliae},
  url          = {http://dx.doi.org/10.1016/j.molimm.2009.11.028},
  volume       = {47},
  year         = {2010},
}