Effect of P availability on temporal dynamics of carbon allocation and Glomus intraradices high-affinity P transporter gene induction in arbuscular mycorrhiza
(2006) In Applied and Environmental Microbiology 72(6). p.4115-4120- Abstract
- Arbuscular mycorrhizal (AM) fungi depend on a C supply from the plant host and simultaneously provide phosphorus to the colonized plant. We therefore evaluated the influence of external P on C allocation in monoxenic Daucus carota-Glomus intraradices cultures in an AM symbiosis. Fungal hyphae proliferated from a solid minimal medium containing colonized roots into a C-free liquid minimal medium with high or low P availability. Roots and hyphae were harvested periodically, and the flow of C from roots to fungus was measured by isotope labeling. We also measured induction of a G. intraradices high-affinity P transporter to estimate fungal P demand. The prevailing hypothesis is that high P availability reduces mycorrhizal fungal growth, but... (More)
- Arbuscular mycorrhizal (AM) fungi depend on a C supply from the plant host and simultaneously provide phosphorus to the colonized plant. We therefore evaluated the influence of external P on C allocation in monoxenic Daucus carota-Glomus intraradices cultures in an AM symbiosis. Fungal hyphae proliferated from a solid minimal medium containing colonized roots into a C-free liquid minimal medium with high or low P availability. Roots and hyphae were harvested periodically, and the flow of C from roots to fungus was measured by isotope labeling. We also measured induction of a G. intraradices high-affinity P transporter to estimate fungal P demand. The prevailing hypothesis is that high P availability reduces mycorrhizal fungal growth, but we found that C How to the fungus was initially highest at the high P level. Only at later harvests, after 100 days of in vitro. culture, were C flow and fungal growth limited at high P availability. Thus, AM fungi can benefit initially from P-enriched environments in terms of plant C allocation. As expected, the P transporter induction was significantly greater at low P availability and greatest in very young mycelia. We found no direct link between C How to the fungus and the P transporter transcription level, which indicates that a good C supply is not essential for induction of the high-affinity P transporter. We describe a mechanism by which P regulates symbiotic C allocation, and we discuss how this mechanism may have evolved in a competitive environment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/159676
- author
- Olsson, Pål Axel LU ; Hansson, Maria LU and Burleigh, Stephen LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 72
- issue
- 6
- pages
- 4115 - 4120
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000238620100039
- pmid:16751522
- scopus:33745159330
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.02154-05
- language
- English
- LU publication?
- yes
- id
- 69d69ae1-b2e9-4400-acf4-1444589b333a (old id 159676)
- date added to LUP
- 2016-04-01 12:09:25
- date last changed
- 2022-01-26 23:36:21
@article{69d69ae1-b2e9-4400-acf4-1444589b333a,
abstract = {{Arbuscular mycorrhizal (AM) fungi depend on a C supply from the plant host and simultaneously provide phosphorus to the colonized plant. We therefore evaluated the influence of external P on C allocation in monoxenic Daucus carota-Glomus intraradices cultures in an AM symbiosis. Fungal hyphae proliferated from a solid minimal medium containing colonized roots into a C-free liquid minimal medium with high or low P availability. Roots and hyphae were harvested periodically, and the flow of C from roots to fungus was measured by isotope labeling. We also measured induction of a G. intraradices high-affinity P transporter to estimate fungal P demand. The prevailing hypothesis is that high P availability reduces mycorrhizal fungal growth, but we found that C How to the fungus was initially highest at the high P level. Only at later harvests, after 100 days of in vitro. culture, were C flow and fungal growth limited at high P availability. Thus, AM fungi can benefit initially from P-enriched environments in terms of plant C allocation. As expected, the P transporter induction was significantly greater at low P availability and greatest in very young mycelia. We found no direct link between C How to the fungus and the P transporter transcription level, which indicates that a good C supply is not essential for induction of the high-affinity P transporter. We describe a mechanism by which P regulates symbiotic C allocation, and we discuss how this mechanism may have evolved in a competitive environment.}},
author = {{Olsson, Pål Axel and Hansson, Maria and Burleigh, Stephen}},
issn = {{0099-2240}},
language = {{eng}},
number = {{6}},
pages = {{4115--4120}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Effect of P availability on temporal dynamics of carbon allocation and Glomus intraradices high-affinity P transporter gene induction in arbuscular mycorrhiza}},
url = {{http://dx.doi.org/10.1128/AEM.02154-05}},
doi = {{10.1128/AEM.02154-05}},
volume = {{72}},
year = {{2006}},
}