The IgD C(H)1 region contains the binding site for the human respiratory pathogen Moraxella catarrhalis IgD-binding protein MID.
Samuelsson, Martin LU ; Jendholm, Johan LU ; Amisten, Stefan LU ; Morrison, Sherie L ; Forsgren, Arne LU and Riesbeck, Kristian LU
(2006)
In European Journal of Immunology
9(Sep;36).
p.2525-2534
- Abstract
- The Moraxella catarrhalis IgD-binding protein (MID) has a unique specificity for human IgD, and the sequence with maximal IgD binding is located within the amino acids MID962-1200. In the present paper, we examined the MID binding site on IgD using a series of recombinant Ig. Full-length IgD, IgD F(ab')(2), and an IgD F(ab') C290R mutant lacking the inter-heavy-chain cysteine 290 were manufactured. Furthermore, a series of IgD/IgG chimeras were constructed. ELISA, dot blot and flow cytometry were used to study the binding of purified Ig to native MID, recombinant MID912-1200 or to Moraxella with or without MID. MID962-1200 bound both the IgD F(ab')(2) and F(ab') C290R, indicating that the binding occurred independently of antibody... (More)
- The Moraxella catarrhalis IgD-binding protein (MID) has a unique specificity for human IgD, and the sequence with maximal IgD binding is located within the amino acids MID962-1200. In the present paper, we examined the MID binding site on IgD using a series of recombinant Ig. Full-length IgD, IgD F(ab')(2), and an IgD F(ab') C290R mutant lacking the inter-heavy-chain cysteine 290 were manufactured. Furthermore, a series of IgD/IgG chimeras were constructed. ELISA, dot blot and flow cytometry were used to study the binding of purified Ig to native MID, recombinant MID912-1200 or to Moraxella with or without MID. MID962-1200 bound both the IgD F(ab')(2) and F(ab') C290R, indicating that the binding occurred independently of antibody structure. When amino acids 157-224 of the IgD C(H)1 region were substituted with IgG sequences, binding by M. catarrhalis or recombinant MID962-1200 was abolished. Subsequent smaller substitutions of IgD C(H)1 157-224 with IgG sequences led us to conclude that IgD C(H)1 amino acids 198-206 were crucial for the interaction between MID and IgD. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/160110
- author
- Samuelsson, Martin
LU
; Jendholm, Johan
LU
; Amisten, Stefan
LU
; Morrison, Sherie L
; Forsgren, Arne
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Moraxella catarrhalis, immunoglobulin D, binding
- in
- European Journal of Immunology
- volume
- 9
- issue
- Sep;36
- pages
- 2525 - 2534
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000240586100026
- pmid:16906531
- scopus:33748857116
- pmid:16906531
- ISSN
- 1521-4141
- DOI
- 10.1002/eji.200535594
- language
- English
- LU publication?
- yes
- id
- 67546171-00d8-4c30-9cda-60dbdbea8c11 (old id 160110)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16906531&dopt=Abstract
- date added to LUP
- 2016-04-01 12:20:54
- date last changed
- 2022-01-27 02:29:24
@article{67546171-00d8-4c30-9cda-60dbdbea8c11,
abstract = {{The Moraxella catarrhalis IgD-binding protein (MID) has a unique specificity for human IgD, and the sequence with maximal IgD binding is located within the amino acids MID962-1200. In the present paper, we examined the MID binding site on IgD using a series of recombinant Ig. Full-length IgD, IgD F(ab')(2), and an IgD F(ab') C290R mutant lacking the inter-heavy-chain cysteine 290 were manufactured. Furthermore, a series of IgD/IgG chimeras were constructed. ELISA, dot blot and flow cytometry were used to study the binding of purified Ig to native MID, recombinant MID912-1200 or to Moraxella with or without MID. MID962-1200 bound both the IgD F(ab')(2) and F(ab') C290R, indicating that the binding occurred independently of antibody structure. When amino acids 157-224 of the IgD C(H)1 region were substituted with IgG sequences, binding by M. catarrhalis or recombinant MID962-1200 was abolished. Subsequent smaller substitutions of IgD C(H)1 157-224 with IgG sequences led us to conclude that IgD C(H)1 amino acids 198-206 were crucial for the interaction between MID and IgD.}},
author = {{Samuelsson, Martin and Jendholm, Johan and Amisten, Stefan and Morrison, Sherie L and Forsgren, Arne and Riesbeck, Kristian}},
issn = {{1521-4141}},
keywords = {{Moraxella catarrhalis; immunoglobulin D; binding}},
language = {{eng}},
number = {{Sep;36}},
pages = {{2525--2534}},
publisher = {{John Wiley & Sons Inc.}},
series = {{European Journal of Immunology}},
title = {{The IgD C(H)1 region contains the binding site for the human respiratory pathogen Moraxella catarrhalis IgD-binding protein MID.}},
url = {{http://dx.doi.org/10.1002/eji.200535594}},
doi = {{10.1002/eji.200535594}},
volume = {{9}},
year = {{2006}},
}