Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta
(2010) In Diabetes Care 33(4). p.754-756- Abstract
- OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting... (More)
- OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1603162
- author
- Burbelo, Peter D. ; Hirai, Hiroki ; Issa, Alexandra T. ; Kingman, Albert ; Lernmark, Åke LU ; Ivarsson, Sten LU ; Notkins, Abner L. and Iadarola, Michael J.
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Diabetes Care
- volume
- 33
- issue
- 4
- pages
- 754 - 756
- publisher
- American Diabetes Association
- external identifiers
-
- wos:000276793200015
- scopus:77952293594
- pmid:20086252
- ISSN
- 1935-5548
- DOI
- 10.2337/dc09-1938
- language
- English
- LU publication?
- yes
- id
- e6a55e8e-c6ea-40df-a3d9-dae69d8524bf (old id 1603162)
- date added to LUP
- 2016-04-01 13:37:04
- date last changed
- 2022-03-21 19:30:33
@article{e6a55e8e-c6ea-40df-a3d9-dae69d8524bf, abstract = {{OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.}}, author = {{Burbelo, Peter D. and Hirai, Hiroki and Issa, Alexandra T. and Kingman, Albert and Lernmark, Åke and Ivarsson, Sten and Notkins, Abner L. and Iadarola, Michael J.}}, issn = {{1935-5548}}, language = {{eng}}, number = {{4}}, pages = {{754--756}}, publisher = {{American Diabetes Association}}, series = {{Diabetes Care}}, title = {{Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta}}, url = {{http://dx.doi.org/10.2337/dc09-1938}}, doi = {{10.2337/dc09-1938}}, volume = {{33}}, year = {{2010}}, }