Elevated neutrophil membrane expression of proteinase 3 is dependent upon CD177 expression.
AbdGawad, Mohamed LU ; Gunnarsson, Lena LU ; Bengtsson, Anders LU ; Geborek, Pierre LU ; Nilsson, L ; Segelmark, Mårten LU and Hellmark, Thomas LU
(2010)
In Clinical and Experimental Immunology
161.
p.89-97
- Abstract
- Summary Proteinase 3 (PR3) is a major autoantigen in anti-neutrophil cytoplasmic antibodies (ANCA)-associated systemic vasculitis (AASV), and the proportion of neutrophils expressing PR3 on their membrane (mPR3(+)) is increased in AASV. We have shown recently that mPR3 and CD177 are expressed on the same cells in healthy individuals. In this study we try to elucidate mechanisms behind the increased mPR3 expression in AASV and its relationship to CD177. All neutrophils in all individuals were either double-positive or double-negative for mPR3 and CD177. The proportion of double-positive neutrophils was increased significantly in AASV and systemic lupus erythematosus patients. The proportion of mPR3(+)/CD177(+) cells was not correlated to... (More)
- Summary Proteinase 3 (PR3) is a major autoantigen in anti-neutrophil cytoplasmic antibodies (ANCA)-associated systemic vasculitis (AASV), and the proportion of neutrophils expressing PR3 on their membrane (mPR3(+)) is increased in AASV. We have shown recently that mPR3 and CD177 are expressed on the same cells in healthy individuals. In this study we try to elucidate mechanisms behind the increased mPR3 expression in AASV and its relationship to CD177. All neutrophils in all individuals were either double-positive or double-negative for mPR3 and CD177. The proportion of double-positive neutrophils was increased significantly in AASV and systemic lupus erythematosus patients. The proportion of mPR3(+)/CD177(+) cells was not correlated to general inflammation, renal function, age, sex, drug treatment and levels of circulating PR3. AASV patients had normal levels of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. Pro-PR3 was found to constitute 10% of circulating PR3 but none of the mPR3. We found increased mRNA levels of both PR3 and CD177 in AASV, but they did not correlate with the proportion of double-positive cells. In cells sorted based on membrane expression, CD177-mRNA was several-fold higher in mPR3(+) cells. When exogenous PR3 was added to CD177-transfected U937 cells, only CD177(+) cells bound PR3 to their membrane. In conclusion, the increased membrane expression of PR3 found in AASV is not linked directly to circulating PR3 or PR3 gene transcription, but is dependent upon CD177 expression and correlated with the transcription of the CD177 gene. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1610038
- author
- AbdGawad, Mohamed
LU
; Gunnarsson, Lena
LU
; Bengtsson, Anders
LU
; Geborek, Pierre
LU
; Nilsson, L
; Segelmark, Mårten
LU
and Hellmark, Thomas
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical and Experimental Immunology
- volume
- 161
- pages
- 89 - 97
- publisher
- British Society for Immunology
- external identifiers
-
- wos:000278396900012
- pmid:20491791
- scopus:77953272041
- ISSN
- 0009-9104
- DOI
- 10.1111/j.1365-2249.2010.04154.x
- language
- English
- LU publication?
- yes
- id
- 40a0c04a-9fca-451b-b638-f7cf5ea15b31 (old id 1610038)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20491791?dopt=Abstract
- date added to LUP
- 2016-04-04 07:58:43
- date last changed
- 2022-03-15 07:41:20
@article{40a0c04a-9fca-451b-b638-f7cf5ea15b31,
abstract = {{Summary Proteinase 3 (PR3) is a major autoantigen in anti-neutrophil cytoplasmic antibodies (ANCA)-associated systemic vasculitis (AASV), and the proportion of neutrophils expressing PR3 on their membrane (mPR3(+)) is increased in AASV. We have shown recently that mPR3 and CD177 are expressed on the same cells in healthy individuals. In this study we try to elucidate mechanisms behind the increased mPR3 expression in AASV and its relationship to CD177. All neutrophils in all individuals were either double-positive or double-negative for mPR3 and CD177. The proportion of double-positive neutrophils was increased significantly in AASV and systemic lupus erythematosus patients. The proportion of mPR3(+)/CD177(+) cells was not correlated to general inflammation, renal function, age, sex, drug treatment and levels of circulating PR3. AASV patients had normal levels of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. Pro-PR3 was found to constitute 10% of circulating PR3 but none of the mPR3. We found increased mRNA levels of both PR3 and CD177 in AASV, but they did not correlate with the proportion of double-positive cells. In cells sorted based on membrane expression, CD177-mRNA was several-fold higher in mPR3(+) cells. When exogenous PR3 was added to CD177-transfected U937 cells, only CD177(+) cells bound PR3 to their membrane. In conclusion, the increased membrane expression of PR3 found in AASV is not linked directly to circulating PR3 or PR3 gene transcription, but is dependent upon CD177 expression and correlated with the transcription of the CD177 gene.}},
author = {{AbdGawad, Mohamed and Gunnarsson, Lena and Bengtsson, Anders and Geborek, Pierre and Nilsson, L and Segelmark, Mårten and Hellmark, Thomas}},
issn = {{0009-9104}},
language = {{eng}},
pages = {{89--97}},
publisher = {{British Society for Immunology}},
series = {{Clinical and Experimental Immunology}},
title = {{Elevated neutrophil membrane expression of proteinase 3 is dependent upon CD177 expression.}},
url = {{http://dx.doi.org/10.1111/j.1365-2249.2010.04154.x}},
doi = {{10.1111/j.1365-2249.2010.04154.x}},
volume = {{161}},
year = {{2010}},
}