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Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2.

Tan, Thuan Tong LU ; Christensen, Jens Jorgen ; Dziegiel, Morten Hanefeld ; Forsgren, Arne LU and Riesbeck, Kristian LU orcid (2006) In Infection and Immunity 74(11). p.6377-6386
Abstract
Morarella catarrhalis immunoglobulin D-binding protein (MID) is a complex antigen with unique immunoglobulin D (IgD)-binding, adhesion, and hernagglutination properties. Previous studies have shown that antibodies raised against MID764-111 in rabbits inhibited M. catarrhalis adhesion to human alveolar epithelial cells, and immunization with MID764-913 resulted in an increased pulmonary clearance in a murine model. Strong immune responses against MID have also consistently been shown in humans. Here, the MID-specified IgG responses were compared to those of ubiquitous surface proteins A1 and A2 (UspA1/A2) using a series of recombinant fragments that spanned all three proteins. Sera were obtained from young children, aged 6 months to 1 year... (More)
Morarella catarrhalis immunoglobulin D-binding protein (MID) is a complex antigen with unique immunoglobulin D (IgD)-binding, adhesion, and hernagglutination properties. Previous studies have shown that antibodies raised against MID764-111 in rabbits inhibited M. catarrhalis adhesion to human alveolar epithelial cells, and immunization with MID764-913 resulted in an increased pulmonary clearance in a murine model. Strong immune responses against MID have also consistently been shown in humans. Here, the MID-specified IgG responses were compared to those of ubiquitous surface proteins A1 and A2 (UspA1/A2) using a series of recombinant fragments that spanned all three proteins. Sera were obtained from young children, aged 6 months to 1 year (n = 8) and 2 to 3 years (n = 15), and healthy adults (n = 16). Acute- and convalescent-phase sera from chronic obstructive pulmonary disease (COPD) patients with M. catarrhalis infective exacerbations (n = 23) were also analyzed. Young children, who are at risk of M. catarrhalis infection, had low levels of anti-MID and anti-UspA1/A2 antibodies. Healthy adults and the majority of COPD patients (16/23) had high levels of antibodies directed against, among others, the adhesive domain of MID and the fibronectin- and C3-binding domains of UspA1/A2. Among eight COPD patients in whom a rise in antibody levels could be detected, these functional domains were also the main regions targeted by the antibodies. In addition, human IgG directed against MID was bactericidal and anti-MID antibodies were additive to antibodies targeting UspA1/A2. Hence, the functional domains in these three antigens may have significant potential in a future vaccine against M. catarrhalis. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
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in
Infection and Immunity
volume
74
issue
11
pages
6377 - 6386
publisher
American Society for Microbiology
external identifiers
  • wos:000241600500041
  • scopus:33750491259
ISSN
1098-5522
DOI
10.1128/IAI.00702-06
language
English
LU publication?
yes
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d18aab91-ce5e-48c5-93f0-7613394ff3f4 (old id 161049)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16966403&dopt=Abstract
date added to LUP
2016-04-01 11:53:00
date last changed
2021-10-06 03:43:10
@article{d18aab91-ce5e-48c5-93f0-7613394ff3f4,
  abstract     = {Morarella catarrhalis immunoglobulin D-binding protein (MID) is a complex antigen with unique immunoglobulin D (IgD)-binding, adhesion, and hernagglutination properties. Previous studies have shown that antibodies raised against MID764-111 in rabbits inhibited M. catarrhalis adhesion to human alveolar epithelial cells, and immunization with MID764-913 resulted in an increased pulmonary clearance in a murine model. Strong immune responses against MID have also consistently been shown in humans. Here, the MID-specified IgG responses were compared to those of ubiquitous surface proteins A1 and A2 (UspA1/A2) using a series of recombinant fragments that spanned all three proteins. Sera were obtained from young children, aged 6 months to 1 year (n = 8) and 2 to 3 years (n = 15), and healthy adults (n = 16). Acute- and convalescent-phase sera from chronic obstructive pulmonary disease (COPD) patients with M. catarrhalis infective exacerbations (n = 23) were also analyzed. Young children, who are at risk of M. catarrhalis infection, had low levels of anti-MID and anti-UspA1/A2 antibodies. Healthy adults and the majority of COPD patients (16/23) had high levels of antibodies directed against, among others, the adhesive domain of MID and the fibronectin- and C3-binding domains of UspA1/A2. Among eight COPD patients in whom a rise in antibody levels could be detected, these functional domains were also the main regions targeted by the antibodies. In addition, human IgG directed against MID was bactericidal and anti-MID antibodies were additive to antibodies targeting UspA1/A2. Hence, the functional domains in these three antigens may have significant potential in a future vaccine against M. catarrhalis.},
  author       = {Tan, Thuan Tong and Christensen, Jens Jorgen and Dziegiel, Morten Hanefeld and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1098-5522},
  language     = {eng},
  number       = {11},
  pages        = {6377--6386},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2.},
  url          = {http://dx.doi.org/10.1128/IAI.00702-06},
  doi          = {10.1128/IAI.00702-06},
  volume       = {74},
  year         = {2006},
}