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Probing control of glucose feeding in Vibrio cholerae cultivations

de Maré, Lena LU ; Andersson, Lena and Hagander, Per LU (2003) In Bioprocess and Biosystems Engineering2000-01-01+01:00 25(4). p.221-228
Abstract
Infection with Vibrio cholerae is a significant problem in manydeveloping countries. Cultivation of Vibrio cholerae is used inproduction of CTB, which is a component in a cholera vaccine. Fed - batch cultivations with V. cholerae in defined mediahave been conducted and reproducible results were obtained. A probing feeding strategy developed by Åkessonfor E. coli cultivations has been tested.The program is working as well for V. cholerae as forE. coliin minimizing the amount acetic acid formed and avoiding anaerobicconditions. Two hours after the feed start most of the acetic acidaccumulated during the batch phase is consumed. The resulting feedrate tends to be thehighest possible with respect to the constraints from cell metabolismand mass... (More)
Infection with Vibrio cholerae is a significant problem in manydeveloping countries. Cultivation of Vibrio cholerae is used inproduction of CTB, which is a component in a cholera vaccine. Fed - batch cultivations with V. cholerae in defined mediahave been conducted and reproducible results were obtained. A probing feeding strategy developed by Åkessonfor E. coli cultivations has been tested.The program is working as well for V. cholerae as forE. coliin minimizing the amount acetic acid formed and avoiding anaerobicconditions. Two hours after the feed start most of the acetic acidaccumulated during the batch phase is consumed. The resulting feedrate tends to be thehighest possible with respect to the constraints from cell metabolismand mass transfer, thus maximizing productivity in terms of biomass.A cell dry weight of 20 - 23 g/l is obtained after12 hours of feeding. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fed-batch cultivations, V. cholerae, probing feeding strategy
in
Bioprocess and Biosystems Engineering2000-01-01+01:00
volume
25
issue
4
pages
221 - 228
publisher
Springer
external identifiers
  • wos:000180866700004
  • scopus:0037253403
ISSN
1615-7605
DOI
language
English
LU publication?
yes
id
a3ac08a8-1f54-4419-9e76-8bc8f091af95 (old id 161684)
date added to LUP
2007-07-04 11:56:13
date last changed
2018-05-29 12:01:58
@article{a3ac08a8-1f54-4419-9e76-8bc8f091af95,
  abstract     = {Infection with Vibrio cholerae is a significant problem in manydeveloping countries. Cultivation of Vibrio cholerae is used inproduction of CTB, which is a component in a cholera vaccine. Fed - batch cultivations with V. cholerae in defined mediahave been conducted and reproducible results were obtained. A probing feeding strategy developed by Åkessonfor E. coli cultivations has been tested.The program is working as well for V. cholerae as forE. coliin minimizing the amount acetic acid formed and avoiding anaerobicconditions. Two hours after the feed start most of the acetic acidaccumulated during the batch phase is consumed. The resulting feedrate tends to be thehighest possible with respect to the constraints from cell metabolismand mass transfer, thus maximizing productivity in terms of biomass.A cell dry weight of 20 - 23 g/l is obtained after12 hours of feeding.},
  author       = {de Maré, Lena and Andersson, Lena and Hagander, Per},
  issn         = {1615-7605},
  keyword      = {fed-batch cultivations,V. cholerae,probing feeding strategy},
  language     = {eng},
  number       = {4},
  pages        = {221--228},
  publisher    = {Springer},
  series       = {Bioprocess and Biosystems Engineering2000-01-01+01:00},
  title        = {Probing control of glucose feeding in Vibrio cholerae cultivations},
  url          = {http://dx.doi.org/},
  volume       = {25},
  year         = {2003},
}