Generation of regionally specified neurons in expanded glial cultures derived from the mouse and human lateral ganglionic eminence
(2001) In Molecular and Cellular Neuroscience 17(5). p.811-820- Abstract
- The specific identity of neuronal precursors within the embryonic brain is, at present, not clear. Here we show that cultures with glial characteristics derived from the embryonic mouse or human lateral ganglionic eminence (LGE) can be expanded over many passages and maintain their glial identity. Interestingly, removal of serum and EGF from the culture medium results in the generation of large numbers of neurons. The neurons derived from these cultures display many characteristic features of striatal neurons, which normally derive from the LGE, even after extensive expansion in vitro. Furthermore, a portion of the neurons generated in these cultures were shown to arise from glial fibrillary acidic protein (GFAP)-expressing cells. These... (More)
- The specific identity of neuronal precursors within the embryonic brain is, at present, not clear. Here we show that cultures with glial characteristics derived from the embryonic mouse or human lateral ganglionic eminence (LGE) can be expanded over many passages and maintain their glial identity. Interestingly, removal of serum and EGF from the culture medium results in the generation of large numbers of neurons. The neurons derived from these cultures display many characteristic features of striatal neurons, which normally derive from the LGE, even after extensive expansion in vitro. Furthermore, a portion of the neurons generated in these cultures were shown to arise from glial fibrillary acidic protein (GFAP)-expressing cells. These results demonstrate that at least a subpopulation of neurogenic LGE precursors exhibit glial characteristics. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1120763
- author
- Skogh, Charlotta ; Eriksson Linsmeier, Cecilia LU ; Kokaia, Merab LU ; Meijer, Xia C. ; Wahlberg, Lars U. ; Wictorin, Klas LU and Campbell, Kenneth LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Neuroscience
- volume
- 17
- issue
- 5
- pages
- 811 - 820
- publisher
- Elsevier
- external identifiers
-
- pmid:11358480
- scopus:0034982058
- pmid:11358480
- ISSN
- 1044-7431
- DOI
- 10.1006/mcne.2001.0973
- language
- English
- LU publication?
- yes
- id
- 162084d4-6cfd-427a-88e4-01b51008bec0 (old id 1120763)
- date added to LUP
- 2016-04-01 12:18:35
- date last changed
- 2022-01-27 01:50:41
@article{162084d4-6cfd-427a-88e4-01b51008bec0, abstract = {{The specific identity of neuronal precursors within the embryonic brain is, at present, not clear. Here we show that cultures with glial characteristics derived from the embryonic mouse or human lateral ganglionic eminence (LGE) can be expanded over many passages and maintain their glial identity. Interestingly, removal of serum and EGF from the culture medium results in the generation of large numbers of neurons. The neurons derived from these cultures display many characteristic features of striatal neurons, which normally derive from the LGE, even after extensive expansion in vitro. Furthermore, a portion of the neurons generated in these cultures were shown to arise from glial fibrillary acidic protein (GFAP)-expressing cells. These results demonstrate that at least a subpopulation of neurogenic LGE precursors exhibit glial characteristics.}}, author = {{Skogh, Charlotta and Eriksson Linsmeier, Cecilia and Kokaia, Merab and Meijer, Xia C. and Wahlberg, Lars U. and Wictorin, Klas and Campbell, Kenneth}}, issn = {{1044-7431}}, language = {{eng}}, number = {{5}}, pages = {{811--820}}, publisher = {{Elsevier}}, series = {{Molecular and Cellular Neuroscience}}, title = {{Generation of regionally specified neurons in expanded glial cultures derived from the mouse and human lateral ganglionic eminence}}, url = {{http://dx.doi.org/10.1006/mcne.2001.0973}}, doi = {{10.1006/mcne.2001.0973}}, volume = {{17}}, year = {{2001}}, }