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Disproportionally low clearance of macromolecules from the plasma to the peritoneal cavity in a mouse model of peritoneal dialysis (PD).

Rippe, Anna LU ; Rippe, Catarina LU ; Swärd, Karl LU and Rippe, Bengt LU (2007) In Nephrology Dialysis Transplantation 22(1). p.88-95
Abstract
Background. This study was performed to establish a model for quantitative measurements of a number of basic peritoneal transport parameters, particularly transperitoneal clearances (Cl) of macromolecules, during mouse peritoneal dialysis.



Methods. Mice were anaesthetized using 3% isofluorane inhalation anaesthesia. The right jugular vein and the left femoral artery were cannulated for infusion and sampling purposes and for registration of (mean) arterial blood pressure. Access to the peritoneal cavity occurred via a thin abdominal catheter (Ø 0.7 mm). About 2.5 ml of either 4% (n = 9) or 1.5% (n = 5) glucose containing PD-fluid were instilled intraperitoneally (i.p.). Dialysate volume was followed vs time using i.p.... (More)
Background. This study was performed to establish a model for quantitative measurements of a number of basic peritoneal transport parameters, particularly transperitoneal clearances (Cl) of macromolecules, during mouse peritoneal dialysis.



Methods. Mice were anaesthetized using 3% isofluorane inhalation anaesthesia. The right jugular vein and the left femoral artery were cannulated for infusion and sampling purposes and for registration of (mean) arterial blood pressure. Access to the peritoneal cavity occurred via a thin abdominal catheter (Ø 0.7 mm). About 2.5 ml of either 4% (n = 9) or 1.5% (n = 5) glucose containing PD-fluid were instilled intraperitoneally (i.p.). Dialysate volume was followed vs time using i.p. RISA (125I human serum albumin) as a volume marker, after correcting for RISA mass disappearance from the peritoneum, assessed separately (n = 11). Microsampling (10 µl) of plasma and dialysate was performed for determinations of glucose, haematocrit, radioactivity (RISA and 51Cr-EDTA) and Ficoll.



Results. The i.p. volume vs time curves [VD(t)] were, after scaling, similar to those observed in humans (and in rats). Clearance of RISA out of the peritoneal cavity (Clout) was 9.33 ± 0.83 µl/min and the clearance of RISA to plasma (Cl->P) and the RISA clearance to the peritoneal cavity (Cl->D) were 1.49 ± 0.13 and 0.084 ± 0.008 µl/min, respectively. The peritoneal transport coefficients for 51Cr-EDTA and glucose, as well as Clout and Cl->P, were 13–17% of those previously assessed in 300 g rats, whereas Cl->D was only ~2% of that in rat.



Conclusions. All peritoneal transport parameters measured, except Cl->D, scaled very well to the corresponding human data. The mechanisms of the disproportionally low clearance of macromolecules from the plasma to the peritoneal cavity in mice remain elusive and warrant further study. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
albumin, capillary permeability, Ficoll, lymph flow, peritoneal dialysis, pore modelling, transcapillary escape rate
in
Nephrology Dialysis Transplantation
volume
22
issue
1
pages
88 - 95
publisher
Oxford University Press
external identifiers
  • wos:000243497200021
  • scopus:33845965003
ISSN
1460-2385
DOI
10.1093/ndt/gfl497
language
English
LU publication?
yes
id
5356012a-83a4-4718-b810-664ba53cff3b (old id 162264)
date added to LUP
2007-07-10 11:32:35
date last changed
2017-07-30 04:45:50
@article{5356012a-83a4-4718-b810-664ba53cff3b,
  abstract     = {Background. This study was performed to establish a model for quantitative measurements of a number of basic peritoneal transport parameters, particularly transperitoneal clearances (Cl) of macromolecules, during mouse peritoneal dialysis.<br/><br>
<br/><br>
Methods. Mice were anaesthetized using 3% isofluorane inhalation anaesthesia. The right jugular vein and the left femoral artery were cannulated for infusion and sampling purposes and for registration of (mean) arterial blood pressure. Access to the peritoneal cavity occurred via a thin abdominal catheter (Ø 0.7 mm). About 2.5 ml of either 4% (n = 9) or 1.5% (n = 5) glucose containing PD-fluid were instilled intraperitoneally (i.p.). Dialysate volume was followed vs time using i.p. RISA (125I human serum albumin) as a volume marker, after correcting for RISA mass disappearance from the peritoneum, assessed separately (n = 11). Microsampling (10 µl) of plasma and dialysate was performed for determinations of glucose, haematocrit, radioactivity (RISA and 51Cr-EDTA) and Ficoll.<br/><br>
<br/><br>
Results. The i.p. volume vs time curves [VD(t)] were, after scaling, similar to those observed in humans (and in rats). Clearance of RISA out of the peritoneal cavity (Clout) was 9.33 ± 0.83 µl/min and the clearance of RISA to plasma (Cl-&gt;P) and the RISA clearance to the peritoneal cavity (Cl-&gt;D) were 1.49 ± 0.13 and 0.084 ± 0.008 µl/min, respectively. The peritoneal transport coefficients for 51Cr-EDTA and glucose, as well as Clout and Cl-&gt;P, were 13–17% of those previously assessed in 300 g rats, whereas Cl-&gt;D was only ~2% of that in rat.<br/><br>
<br/><br>
Conclusions. All peritoneal transport parameters measured, except Cl-&gt;D, scaled very well to the corresponding human data. The mechanisms of the disproportionally low clearance of macromolecules from the plasma to the peritoneal cavity in mice remain elusive and warrant further study.},
  author       = {Rippe, Anna and Rippe, Catarina and Swärd, Karl and Rippe, Bengt},
  issn         = {1460-2385},
  keyword      = {albumin,capillary permeability,Ficoll,lymph flow,peritoneal dialysis,pore modelling,transcapillary escape rate},
  language     = {eng},
  number       = {1},
  pages        = {88--95},
  publisher    = {Oxford University Press},
  series       = {Nephrology Dialysis Transplantation},
  title        = {Disproportionally low clearance of macromolecules from the plasma to the peritoneal cavity in a mouse model of peritoneal dialysis (PD).},
  url          = {http://dx.doi.org/10.1093/ndt/gfl497},
  volume       = {22},
  year         = {2007},
}