Fast Surface Acoustic Wave-Matrix-Assisted Laser Desorption Ionization Mass Spectrometry of Cell Response from Islets of Langerhans.
Bllaci, Loreta ; Kjellström, Sven LU ; Eliasson, Lena LU
; Friend, James R
; Yeo, Leslie Y
and Nilsson, Staffan
LU
(2013)
In Analytical Chemistry
85(5).
p.2623-2629
- Abstract
- A desire for higher speed and performance in molecular profiling analysis at a reduced cost is driving a trend in miniaturization and simplification of procedures. Here we report the use of a surface acoustic wave (SAW) atomizer for fast sample handling in matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) peptide and protein profiling of Islets of Langerhans, for future type 2 diabetes (T2D) studies. Here the SAW atomizer was used for ultrasound (acoustic) extraction of insulin and other peptide hormones released from freshly prepared islets, stimulated directly on a membrane. A high energy propagating SAW atomizes the membrane-bound liquid into approximately 2 μm diameter droplets, rich in cell-released molecules.... (More)
- A desire for higher speed and performance in molecular profiling analysis at a reduced cost is driving a trend in miniaturization and simplification of procedures. Here we report the use of a surface acoustic wave (SAW) atomizer for fast sample handling in matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) peptide and protein profiling of Islets of Langerhans, for future type 2 diabetes (T2D) studies. Here the SAW atomizer was used for ultrasound (acoustic) extraction of insulin and other peptide hormones released from freshly prepared islets, stimulated directly on a membrane. A high energy propagating SAW atomizes the membrane-bound liquid into approximately 2 μm diameter droplets, rich in cell-released molecules. Besides acting as a sample carrier, the membrane provides a purification step by entrapping cell clusters and other impurities within its fibers. A new SAW-based sample-matrix deposition method for MALDI MS was developed and characterized by a strong insulin signal, and a limit of detection (LOD) lower than 100 amol was achieved. Our results support previous work reporting the SAW atomizer as a fast and inexpensive tool for ultrasound, membrane-based sample extraction. When interfaced with MALDI MS, the SAW atomizer constitutes a valuable tool for rapid cell studies. Other biomedical applications of SAW-MALDI MS are currently being developed, aiming at fast profiling of biofluids. The membrane sampling is a simplistic and noninvasive collection method of limited volume biofluids such as the gingival fluid and the tearfilm. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3560180
- author
- Bllaci, Loreta
; Kjellström, Sven
LU
; Eliasson, Lena
LU
; Friend, James R
; Yeo, Leslie Y
and Nilsson, Staffan
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 85
- issue
- 5
- pages
- 2623 - 2629
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000317031600013
- pmid:23384197
- scopus:84874606427
- pmid:23384197
- ISSN
- 1520-6882
- DOI
- 10.1021/ac3019125
- language
- English
- LU publication?
- yes
- id
- 163a6b6a-bbb6-4e76-ba2e-566d423cc970 (old id 3560180)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23384197?dopt=Abstract
- date added to LUP
- 2016-04-01 10:12:50
- date last changed
- 2022-04-27 19:49:09
@article{163a6b6a-bbb6-4e76-ba2e-566d423cc970,
abstract = {{A desire for higher speed and performance in molecular profiling analysis at a reduced cost is driving a trend in miniaturization and simplification of procedures. Here we report the use of a surface acoustic wave (SAW) atomizer for fast sample handling in matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) peptide and protein profiling of Islets of Langerhans, for future type 2 diabetes (T2D) studies. Here the SAW atomizer was used for ultrasound (acoustic) extraction of insulin and other peptide hormones released from freshly prepared islets, stimulated directly on a membrane. A high energy propagating SAW atomizes the membrane-bound liquid into approximately 2 μm diameter droplets, rich in cell-released molecules. Besides acting as a sample carrier, the membrane provides a purification step by entrapping cell clusters and other impurities within its fibers. A new SAW-based sample-matrix deposition method for MALDI MS was developed and characterized by a strong insulin signal, and a limit of detection (LOD) lower than 100 amol was achieved. Our results support previous work reporting the SAW atomizer as a fast and inexpensive tool for ultrasound, membrane-based sample extraction. When interfaced with MALDI MS, the SAW atomizer constitutes a valuable tool for rapid cell studies. Other biomedical applications of SAW-MALDI MS are currently being developed, aiming at fast profiling of biofluids. The membrane sampling is a simplistic and noninvasive collection method of limited volume biofluids such as the gingival fluid and the tearfilm.}},
author = {{Bllaci, Loreta and Kjellström, Sven and Eliasson, Lena and Friend, James R and Yeo, Leslie Y and Nilsson, Staffan}},
issn = {{1520-6882}},
language = {{eng}},
number = {{5}},
pages = {{2623--2629}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Fast Surface Acoustic Wave-Matrix-Assisted Laser Desorption Ionization Mass Spectrometry of Cell Response from Islets of Langerhans.}},
url = {{http://dx.doi.org/10.1021/ac3019125}},
doi = {{10.1021/ac3019125}},
volume = {{85}},
year = {{2013}},
}