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Comparison of measurements of autoantibodies to glutamic acid decarboxylase and islet antigen-2 in whole blood eluates from dried blood spots using the RSR-enzyme linked immunosorbent assay kits and in-house radioimmunoassays.

Persson, Anders; Becker, Charlotte LU ; Jönsson, Ida LU ; Ramelius, Anita LU and Törn, Carina LU (2010) In Experimental Diabetes Research 2010.
Abstract
To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; P = .008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; P < .001). Negative or low positive patients and control samples in the... (More)
To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; P = .008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; P < .001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Diabetes Research
volume
2010
publisher
Hindawi Publishing Corporation
external identifiers
  • wos:000279768100001
  • pmid:20613950
  • scopus:77957284615
ISSN
1687-5214
DOI
10.1155/2010/173652
language
English
LU publication?
yes
id
d425816a-3fc0-4967-b2e4-9e4195f18fc3 (old id 1645210)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20613950?dopt=Abstract
date added to LUP
2010-08-02 11:03:18
date last changed
2018-05-29 10:22:54
@article{d425816a-3fc0-4967-b2e4-9e4195f18fc3,
  abstract     = {To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; P = .008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; P &lt; .001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.},
  articleno    = {173652},
  author       = {Persson, Anders and Becker, Charlotte and Jönsson, Ida and Ramelius, Anita and Törn, Carina},
  issn         = {1687-5214},
  language     = {eng},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Experimental Diabetes Research},
  title        = {Comparison of measurements of autoantibodies to glutamic acid decarboxylase and islet antigen-2 in whole blood eluates from dried blood spots using the RSR-enzyme linked immunosorbent assay kits and in-house radioimmunoassays.},
  url          = {http://dx.doi.org/10.1155/2010/173652},
  volume       = {2010},
  year         = {2010},
}