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GPR40 is expressed in glucagon producing cells and affects glucagon secretion.

Flodgren, Erik LU ; Olde, Björn LU ; Meidute, Sandra LU ; Sörhede Winzell, Maria LU ; Ahrén, Bo LU and Salehi, S Albert LU (2007) In Biochemical and Biophysical Research Communications 354(1). p.240-245
Abstract
The free fatty acid receptor, GPR40, has been coupled with insulin secretion via its expression in pancreatic beta-cells. However, the role of GPR40 in the release of glucagon has not been studied and previous attempts to identify the receptor in alpha-cells have been unfruitful. Using double-staining for glucagon and GPR40 expression, we demonstrate that the two are expressed in the same cells in the periphery of mouse islets. In-R1-G9 hamster glucagonoma cells respond dose-dependently to linoleic acid stimulation by elevated phosphatidyl inositol hydrolysis and glucagon release and the cells become increasingly responsive to fatty acid stimulation when overexpressing GPR40. Isolated mouse islets also secrete glucagon in response to... (More)
The free fatty acid receptor, GPR40, has been coupled with insulin secretion via its expression in pancreatic beta-cells. However, the role of GPR40 in the release of glucagon has not been studied and previous attempts to identify the receptor in alpha-cells have been unfruitful. Using double-staining for glucagon and GPR40 expression, we demonstrate that the two are expressed in the same cells in the periphery of mouse islets. In-R1-G9 hamster glucagonoma cells respond dose-dependently to linoleic acid stimulation by elevated phosphatidyl inositol hydrolysis and glucagon release and the cells become increasingly responsive to fatty acid stimulation when overexpressing GPR40. Isolated mouse islets also secrete glucagon in response to linoleic acid, a response that was abolished by antisense treatment against GPR40. This study demonstrates that GPR40 is present and active in pancreatic alpha-cells and puts further emphasis on the importance of this nutrient sensing receptor in islet function. (c) 2006 Elsevier Inc. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
pancreatic islets, diabetes, glucagon, alpha-cell, GPR40, free fatty acid
in
Biochemical and Biophysical Research Communications
volume
354
issue
1
pages
240 - 245
publisher
Elsevier
external identifiers
  • wos:000244133800040
  • scopus:33846327180
ISSN
1090-2104
DOI
10.1016/j.bbrc.2006.12.193
language
English
LU publication?
yes
id
1b09cc6c-61d1-4d85-a2ea-4d3db4c6615c (old id 165006)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17214971&dopt=Abstract
date added to LUP
2007-07-09 14:42:20
date last changed
2017-07-23 04:46:18
@article{1b09cc6c-61d1-4d85-a2ea-4d3db4c6615c,
  abstract     = {The free fatty acid receptor, GPR40, has been coupled with insulin secretion via its expression in pancreatic beta-cells. However, the role of GPR40 in the release of glucagon has not been studied and previous attempts to identify the receptor in alpha-cells have been unfruitful. Using double-staining for glucagon and GPR40 expression, we demonstrate that the two are expressed in the same cells in the periphery of mouse islets. In-R1-G9 hamster glucagonoma cells respond dose-dependently to linoleic acid stimulation by elevated phosphatidyl inositol hydrolysis and glucagon release and the cells become increasingly responsive to fatty acid stimulation when overexpressing GPR40. Isolated mouse islets also secrete glucagon in response to linoleic acid, a response that was abolished by antisense treatment against GPR40. This study demonstrates that GPR40 is present and active in pancreatic alpha-cells and puts further emphasis on the importance of this nutrient sensing receptor in islet function. (c) 2006 Elsevier Inc. All rights reserved.},
  author       = {Flodgren, Erik and Olde, Björn and Meidute, Sandra and Sörhede Winzell, Maria and Ahrén, Bo and Salehi, S Albert},
  issn         = {1090-2104},
  keyword      = {pancreatic islets,diabetes,glucagon,alpha-cell,GPR40,free fatty acid},
  language     = {eng},
  number       = {1},
  pages        = {240--245},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {GPR40 is expressed in glucagon producing cells and affects glucagon secretion.},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2006.12.193},
  volume       = {354},
  year         = {2007},
}