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Functional in vitro characterisation of the androgen receptor GGN polymorphism.

Lundin, Kristina LU ; Giwercman, Aleksander LU ; Dizeyi, Nishtman LU and Giwercman, Yvonne LU (2007) In Molecular and Cellular Endocrinology 264(1-2). p.184-187
Abstract
Superior androgen receptor (AR) function in subjects carrying a GGN repeat length of 23 (GGN23) has been indicated in vivo. Therefore, the activity of the AR carrying GGN23 combined with CAG22 was compared to the AR with GGN10, 24 and 27, respectively, in the presence of 0.1-100 nM testosterone or DHT. At 100 nM DHT, GGN24 showed 35% lower transactivating activity (95% [CI]: 20-50%) than GGN23. GGN10 and GGN27 also showed significantly less AR activity than GGN23 (mean differences [95% CI]: 54% [40-68%] and 58% [39-78%], respectively). The same trend was also observed at lower DHT concentrations. In response to R 188 1, GGN23 activity was significantly higher than for other lengths. ARs with other glutamine numbers than 23 have lower... (More)
Superior androgen receptor (AR) function in subjects carrying a GGN repeat length of 23 (GGN23) has been indicated in vivo. Therefore, the activity of the AR carrying GGN23 combined with CAG22 was compared to the AR with GGN10, 24 and 27, respectively, in the presence of 0.1-100 nM testosterone or DHT. At 100 nM DHT, GGN24 showed 35% lower transactivating activity (95% [CI]: 20-50%) than GGN23. GGN10 and GGN27 also showed significantly less AR activity than GGN23 (mean differences [95% CI]: 54% [40-68%] and 58% [39-78%], respectively). The same trend was also observed at lower DHT concentrations. In response to R 188 1, GGN23 activity was significantly higher than for other lengths. ARs with other glutamine numbers than 23 have lower transactivating capacity in response to both testosterone and DHT. Congenital malformations and other signs of hypoandrogenism in subjects with AR gene GGN lengths other than 23 could, hence, be related to a lower AR activity. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polymorphism, androgen receptor, transcription
in
Molecular and Cellular Endocrinology
volume
264
issue
1-2
pages
184 - 187
publisher
Elsevier
external identifiers
  • wos:000244157800022
  • scopus:33846257723
ISSN
1872-8057
DOI
10.1016/j.mce.2006.11.008
language
English
LU publication?
yes
id
b268b326-27c7-49a0-a312-967143442017 (old id 165196)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17197074&dopt=Abstract
date added to LUP
2007-07-26 09:47:06
date last changed
2017-10-22 03:38:41
@article{b268b326-27c7-49a0-a312-967143442017,
  abstract     = {Superior androgen receptor (AR) function in subjects carrying a GGN repeat length of 23 (GGN23) has been indicated in vivo. Therefore, the activity of the AR carrying GGN23 combined with CAG22 was compared to the AR with GGN10, 24 and 27, respectively, in the presence of 0.1-100 nM testosterone or DHT. At 100 nM DHT, GGN24 showed 35% lower transactivating activity (95% [CI]: 20-50%) than GGN23. GGN10 and GGN27 also showed significantly less AR activity than GGN23 (mean differences [95% CI]: 54% [40-68%] and 58% [39-78%], respectively). The same trend was also observed at lower DHT concentrations. In response to R 188 1, GGN23 activity was significantly higher than for other lengths. ARs with other glutamine numbers than 23 have lower transactivating capacity in response to both testosterone and DHT. Congenital malformations and other signs of hypoandrogenism in subjects with AR gene GGN lengths other than 23 could, hence, be related to a lower AR activity.},
  author       = {Lundin, Kristina and Giwercman, Aleksander and Dizeyi, Nishtman and Giwercman, Yvonne},
  issn         = {1872-8057},
  keyword      = {polymorphism,androgen receptor,transcription},
  language     = {eng},
  number       = {1-2},
  pages        = {184--187},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Endocrinology},
  title        = {Functional in vitro characterisation of the androgen receptor GGN polymorphism.},
  url          = {http://dx.doi.org/10.1016/j.mce.2006.11.008},
  volume       = {264},
  year         = {2007},
}