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Environmental influences on exopolysaccharide formation in Lactobacillus reuteri ATCC 55730.

Årsköld, Emma LU ; Svensson, Malin LU ; Grage, Halfdan LU ; Roos, Stefan; Rådström, Peter LU and van Niel, Ed LU (2007) In International Journal of Food Microbiology 116(1). p.159-167
Abstract
Lactobacillus reuteri is known to produce exopolysaccharides (EPS), which have the potential to be used as an alternative biothickener in the food industry. In this study, the effect of several environmental conditions on the growth and EPS production in the L. reuteri strain ATCC 55730 was determined. The expression of the corresponding reuteransucrase gene, gtfO, was investigated over time and the results indicated that the expression increased with growth during the exponential phase and subsequently decreased in the stationary phase. Fermentation with glucose and/or sucrose as carbon and energy source revealed that gtfO was constitutively expressed and that the activity profile was independent of the sugar source. In the applied ranges... (More)
Lactobacillus reuteri is known to produce exopolysaccharides (EPS), which have the potential to be used as an alternative biothickener in the food industry. In this study, the effect of several environmental conditions on the growth and EPS production in the L. reuteri strain ATCC 55730 was determined. The expression of the corresponding reuteransucrase gene, gtfO, was investigated over time and the results indicated that the expression increased with growth during the exponential phase and subsequently decreased in the stationary phase. Fermentation with glucose and/or sucrose as carbon and energy source revealed that gtfO was constitutively expressed and that the activity profile was independent of the sugar source. In the applied ranges of parameter values, temperature and pH were the most important factors for EPS formation and only temperature for growth. The best EPS yield, 1.4 g g(-1) CDW, was obtained at the conditions 37 degrees C, pH 4.5 and 100 g l(-1) sucrose, which were close to the estimated optimal conditions: pH 4.56 and 100 g l(-1) sucrose. No EPS formation could be detected with glucose. In addition, no direct connection between the expression and the activity of reuteransucrase could be established. Finally, the strain ATCC 55730 was benchmarked against 14 other L. reuteri strains with respect to EPS production from sucrose and abilities to metabolise sucrose, glucose and fructose. Eight strains were able to produce glucan and a corresponding glucansucrase gene was confirmed for each of them. (c) 2007 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glucansucrase, hornopolysaccharides, exopolysaccharide, Lactobacillus reuteri, gene expression
in
International Journal of Food Microbiology
volume
116
issue
1
pages
159 - 167
publisher
Elsevier
external identifiers
  • wos:000246240000021
  • scopus:33947591533
ISSN
0168-1605
DOI
10.1016/j.ijfoodmicro.2006.12.010
language
English
LU publication?
yes
id
b0a1b2ec-40a8-456c-ad5e-1104fc9bf16f (old id 165490)
date added to LUP
2007-07-11 12:47:23
date last changed
2017-01-01 05:13:09
@article{b0a1b2ec-40a8-456c-ad5e-1104fc9bf16f,
  abstract     = {Lactobacillus reuteri is known to produce exopolysaccharides (EPS), which have the potential to be used as an alternative biothickener in the food industry. In this study, the effect of several environmental conditions on the growth and EPS production in the L. reuteri strain ATCC 55730 was determined. The expression of the corresponding reuteransucrase gene, gtfO, was investigated over time and the results indicated that the expression increased with growth during the exponential phase and subsequently decreased in the stationary phase. Fermentation with glucose and/or sucrose as carbon and energy source revealed that gtfO was constitutively expressed and that the activity profile was independent of the sugar source. In the applied ranges of parameter values, temperature and pH were the most important factors for EPS formation and only temperature for growth. The best EPS yield, 1.4 g g(-1) CDW, was obtained at the conditions 37 degrees C, pH 4.5 and 100 g l(-1) sucrose, which were close to the estimated optimal conditions: pH 4.56 and 100 g l(-1) sucrose. No EPS formation could be detected with glucose. In addition, no direct connection between the expression and the activity of reuteransucrase could be established. Finally, the strain ATCC 55730 was benchmarked against 14 other L. reuteri strains with respect to EPS production from sucrose and abilities to metabolise sucrose, glucose and fructose. Eight strains were able to produce glucan and a corresponding glucansucrase gene was confirmed for each of them. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Årsköld, Emma and Svensson, Malin and Grage, Halfdan and Roos, Stefan and Rådström, Peter and van Niel, Ed},
  issn         = {0168-1605},
  keyword      = {glucansucrase,hornopolysaccharides,exopolysaccharide,Lactobacillus reuteri,gene expression},
  language     = {eng},
  number       = {1},
  pages        = {159--167},
  publisher    = {Elsevier},
  series       = {International Journal of Food Microbiology},
  title        = {Environmental influences on exopolysaccharide formation in Lactobacillus reuteri ATCC 55730.},
  url          = {http://dx.doi.org/10.1016/j.ijfoodmicro.2006.12.010},
  volume       = {116},
  year         = {2007},
}