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Thyrostimulin (a TSH-like Hormone) Expression in Orbital and Thyroid Tissue.

Lantz, Mikael LU ; Planck, Tereza LU ; Capretz, Annika; Nilsson, Emma; Frenander, Christofer LU ; Bondeson, Anne-Greth LU ; Ridderstråle, Martin LU ; Åberg, Magnus LU ; Åsman, Peter LU and Groop, Leif LU , et al. (2007) In Thyroid 17(2). p.113-118
Abstract
Objective: To investigate gene expression of thyrostimulin in orbital and thyroid tissue from patients with and without Graves' disease. Design: Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used for detection of thyrostimulin gene expression in intraorbital adipose tissue from patients with severe ophthalmopathy and thyroid healthy controls in addition to thyrostimulin expression in normal thyroid tissue, multinodular goiter tissue, and Graves' thyroid tissue. Main Outcome: In intraorbital tissue, thyrostimulin expression was identified in both patients and controls with fluorescence intensities varying between 0.23 and 0.88 in patients and 0.29 and 8.9 in controls before treatment with DNase. The signal of... (More)
Objective: To investigate gene expression of thyrostimulin in orbital and thyroid tissue from patients with and without Graves' disease. Design: Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used for detection of thyrostimulin gene expression in intraorbital adipose tissue from patients with severe ophthalmopathy and thyroid healthy controls in addition to thyrostimulin expression in normal thyroid tissue, multinodular goiter tissue, and Graves' thyroid tissue. Main Outcome: In intraorbital tissue, thyrostimulin expression was identified in both patients and controls with fluorescence intensities varying between 0.23 and 0.88 in patients and 0.29 and 8.9 in controls before treatment with DNase. The signal of thyrostimulin was weak or absent in intraorbital adipose tissue from patients with ophthalmopathy and thyroid healthy controls after treatment of samples with DNase. This was in contrast to the expression of the thyroid-stimulating hormone (TSH) receptor and the housekeeping gene cyclophilin A that were detected both before and after DNase treatment. Similar results were found when analyzing human and rat thyroid tissue. Conclusions: Neither did we demonstrate gene expression of thyrostimulin in intraorbital adipose tissue or in thyroid tissue, nor could we confirm earlier findings in rat thyroid tissue. Whether thyrostimulin is a regulator of thyroid function has to be further investigated in future studies. (Less)
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Contribution to journal
publication status
published
subject
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Thyroid
volume
17
issue
2
pages
113 - 118
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000244642400005
  • scopus:33847663005
ISSN
1557-9077
DOI
10.1089/thy.2006.0155
language
English
LU publication?
yes
id
f3f94f39-c45f-488f-b710-f23f4cdef643 (old id 165498)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17316112&dopt=Abstract
date added to LUP
2007-07-25 09:23:53
date last changed
2017-07-09 04:18:34
@article{f3f94f39-c45f-488f-b710-f23f4cdef643,
  abstract     = {Objective: To investigate gene expression of thyrostimulin in orbital and thyroid tissue from patients with and without Graves' disease. Design: Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used for detection of thyrostimulin gene expression in intraorbital adipose tissue from patients with severe ophthalmopathy and thyroid healthy controls in addition to thyrostimulin expression in normal thyroid tissue, multinodular goiter tissue, and Graves' thyroid tissue. Main Outcome: In intraorbital tissue, thyrostimulin expression was identified in both patients and controls with fluorescence intensities varying between 0.23 and 0.88 in patients and 0.29 and 8.9 in controls before treatment with DNase. The signal of thyrostimulin was weak or absent in intraorbital adipose tissue from patients with ophthalmopathy and thyroid healthy controls after treatment of samples with DNase. This was in contrast to the expression of the thyroid-stimulating hormone (TSH) receptor and the housekeeping gene cyclophilin A that were detected both before and after DNase treatment. Similar results were found when analyzing human and rat thyroid tissue. Conclusions: Neither did we demonstrate gene expression of thyrostimulin in intraorbital adipose tissue or in thyroid tissue, nor could we confirm earlier findings in rat thyroid tissue. Whether thyrostimulin is a regulator of thyroid function has to be further investigated in future studies.},
  author       = {Lantz, Mikael and Planck, Tereza and Capretz, Annika and Nilsson, Emma and Frenander, Christofer and Bondeson, Anne-Greth and Ridderstråle, Martin and Åberg, Magnus and Åsman, Peter and Groop, Leif and Hallengren, Bengt},
  issn         = {1557-9077},
  language     = {eng},
  number       = {2},
  pages        = {113--118},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Thyroid},
  title        = {Thyrostimulin (a TSH-like Hormone) Expression in Orbital and Thyroid Tissue.},
  url          = {http://dx.doi.org/10.1089/thy.2006.0155},
  volume       = {17},
  year         = {2007},
}