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High Proportions of Proinflammatory Bacteria on the Colonic Mucosa in a Young Patient with Ulcerative Colitis as Revealed by Cloning and Sequencing of 16S rRNA Genes.

Wang, Mei LU ; Molin, Göran LU ; Ahrné, Siv LU ; Adawi, Diya LU and Jeppsson, Bengt LU (2007) In Digestive Diseases and Sciences 52. p.620-627
Abstract
The pathogenesis of ulcerative colitis (UC) remains unknown. It is thought to be due to an abnormal and uncontrolled immune response to normally occurring constituents of the intestine. Microbial agents appear to be involved in the pathogenesis and intestinal bacteria seem to be an important factor in the development and chronicity. The aim of this study was to investigate the colonic microbiota of a patient with UC. The colonic tissues were taken during surgery from a 12-year-old girl suffering from UC. The microbiota on the colonic samples was studied by cloning and sequencing of amplified 16S rRNA genes. Compared with healthy subjects, alteration of the dominant bacterial group was observed in the UC patient. We found a high incidence... (More)
The pathogenesis of ulcerative colitis (UC) remains unknown. It is thought to be due to an abnormal and uncontrolled immune response to normally occurring constituents of the intestine. Microbial agents appear to be involved in the pathogenesis and intestinal bacteria seem to be an important factor in the development and chronicity. The aim of this study was to investigate the colonic microbiota of a patient with UC. The colonic tissues were taken during surgery from a 12-year-old girl suffering from UC. The microbiota on the colonic samples was studied by cloning and sequencing of amplified 16S rRNA genes. Compared with healthy subjects, alteration of the dominant bacterial group was observed in the UC patient. We found a high incidence of Enterobacteriaceae, Bacteroides fragilis, and the single phylotype of the Faecalibacterium prausnitzii-like "Butyrate-producing bacterium" L2-6. Furthermore, there was a substantial presence of Pseudomonas aeruginosa in the present case of UC. The high proportion of adverse proinflammatory species is striking in the present case compared with more normal situations. Even if those bacteria are not the cause of the UC, they most probably enhance the symptoms of the disease. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cloning and, sequencing, colonic microbiota, ulcerative colitis, inflammatory bowel disease, 16S rRNA gene
in
Digestive Diseases and Sciences
volume
52
pages
620 - 627
publisher
Springer
external identifiers
  • wos:000244306500003
  • scopus:33847200125
ISSN
1573-2568
DOI
10.1007/s10620-006-9461-1
language
English
LU publication?
yes
id
871917a9-e292-490f-8aa9-d2b6333d8bfe (old id 165997)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17265126&dopt=Abstract
date added to LUP
2007-07-30 14:42:36
date last changed
2017-11-19 03:30:58
@article{871917a9-e292-490f-8aa9-d2b6333d8bfe,
  abstract     = {The pathogenesis of ulcerative colitis (UC) remains unknown. It is thought to be due to an abnormal and uncontrolled immune response to normally occurring constituents of the intestine. Microbial agents appear to be involved in the pathogenesis and intestinal bacteria seem to be an important factor in the development and chronicity. The aim of this study was to investigate the colonic microbiota of a patient with UC. The colonic tissues were taken during surgery from a 12-year-old girl suffering from UC. The microbiota on the colonic samples was studied by cloning and sequencing of amplified 16S rRNA genes. Compared with healthy subjects, alteration of the dominant bacterial group was observed in the UC patient. We found a high incidence of Enterobacteriaceae, Bacteroides fragilis, and the single phylotype of the Faecalibacterium prausnitzii-like "Butyrate-producing bacterium" L2-6. Furthermore, there was a substantial presence of Pseudomonas aeruginosa in the present case of UC. The high proportion of adverse proinflammatory species is striking in the present case compared with more normal situations. Even if those bacteria are not the cause of the UC, they most probably enhance the symptoms of the disease.},
  author       = {Wang, Mei and Molin, Göran and Ahrné, Siv and Adawi, Diya and Jeppsson, Bengt},
  issn         = {1573-2568},
  keyword      = {cloning and,sequencing,colonic microbiota,ulcerative colitis,inflammatory bowel disease,16S rRNA gene},
  language     = {eng},
  pages        = {620--627},
  publisher    = {Springer},
  series       = {Digestive Diseases and Sciences},
  title        = {High Proportions of Proinflammatory Bacteria on the Colonic Mucosa in a Young Patient with Ulcerative Colitis as Revealed by Cloning and Sequencing of 16S rRNA Genes.},
  url          = {http://dx.doi.org/10.1007/s10620-006-9461-1},
  volume       = {52},
  year         = {2007},
}