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In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra.

Ekblad, Lars LU ; Baldetorp, Bo LU ; Fernö, Mårten LU ; Olsson, Håkan LU and Welinder, Charlotte LU (2007) In Journal of Proteome Research 6(4). p.1609-1614
Abstract
SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteome Research
volume
6
issue
4
pages
1609 - 1614
publisher
The American Chemical Society
external identifiers
  • wos:000245510900038
  • scopus:34248221112
ISSN
1535-3893
DOI
10.1021/pr060633y
language
English
LU publication?
yes
id
f5b8d001-c8ca-4cbf-8fc7-dec205e325e6 (old id 166341)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17373843&dopt=Abstract
date added to LUP
2007-07-23 08:41:57
date last changed
2017-08-24 14:33:58
@article{f5b8d001-c8ca-4cbf-8fc7-dec205e325e6,
  abstract     = {SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.},
  author       = {Ekblad, Lars and Baldetorp, Bo and Fernö, Mårten and Olsson, Håkan and Welinder, Charlotte},
  issn         = {1535-3893},
  language     = {eng},
  number       = {4},
  pages        = {1609--1614},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra.},
  url          = {http://dx.doi.org/10.1021/pr060633y},
  volume       = {6},
  year         = {2007},
}