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The importance of fibroblasts in remodelling of the human uterine cervix during pregnancy and parturition.

Malmström, Erik LU ; Sennstrom, Maria; Holmberg, Anna LU ; Frielingsdorf, Helena LU ; Eklund, Erik LU ; Malmstrom, Lars; Tufvesson, Ellen LU ; Gomez, Maria LU ; Westergren-Thorsson, Gunilla LU and Ekman-Ordeberg, Gunvor, et al. (2007) In Molecular Human Reproduction 13. p.333-341
Abstract
It is well established that fibroblasts play a crucial role in pathophysiological extracellular matrix remodelling. The aim of this project is to elucidate their role in normal physiological remodelling. Specifically, the remodelling of the human cervix during pregnancy, resulting in an enabled passage of the child, is used as the model system. Fibroblast cultures were established from cervices of non-pregnant women, women after 36 weeks of pregnancy and women directly after partus. The cells were immunostained and quantified by western blots for differentiation markers. The cultures were screened for cytokine and metalloproteinase production and characterized by global proteome analysis. The cell cultures established from partal donors... (More)
It is well established that fibroblasts play a crucial role in pathophysiological extracellular matrix remodelling. The aim of this project is to elucidate their role in normal physiological remodelling. Specifically, the remodelling of the human cervix during pregnancy, resulting in an enabled passage of the child, is used as the model system. Fibroblast cultures were established from cervices of non-pregnant women, women after 36 weeks of pregnancy and women directly after partus. The cells were immunostained and quantified by western blots for differentiation markers. The cultures were screened for cytokine and metalloproteinase production and characterized by global proteome analysis. The cell cultures established from partal donors differ significantly from those from non-pregnant donors, which is in accordance with in vivo findings. A decrease in alpha-smooth actin and prolyl-4hydroxylase and an increase in interleukin (IL)-6, IL-8 and matrix metalloproteinases (MMP)-1 and MMP-3 were observed in cultures from partal donors. 2D-gel electrophoresis followed by mass spectrometry showed that the expression of 59 proteins was changed significantly in cultures of partal donors. The regulated proteins are involved in protein kinase C signalling, Ca2+ binding, cytoskeletal organization, angiogenesis and degradation. Our data suggest that remodelling of the human cervix is orchestrated by fibroblasts, which are activated or recruited by the inflammatory processes occurring during the ripening cascade. (Less)
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Molecular Human Reproduction
volume
13
pages
333 - 341
publisher
Oxford University Press
external identifiers
  • wos:000247348500006
  • scopus:34447339976
ISSN
1460-2407
DOI
10.1093/molehr/gal117
language
English
LU publication?
yes
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253dcdb9-cc24-4e65-9b1b-28e25071ca99 (old id 166830)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17337476&dopt=Abstract
date added to LUP
2007-07-11 12:19:55
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2017-03-26 03:28:31
@article{253dcdb9-cc24-4e65-9b1b-28e25071ca99,
  abstract     = {It is well established that fibroblasts play a crucial role in pathophysiological extracellular matrix remodelling. The aim of this project is to elucidate their role in normal physiological remodelling. Specifically, the remodelling of the human cervix during pregnancy, resulting in an enabled passage of the child, is used as the model system. Fibroblast cultures were established from cervices of non-pregnant women, women after 36 weeks of pregnancy and women directly after partus. The cells were immunostained and quantified by western blots for differentiation markers. The cultures were screened for cytokine and metalloproteinase production and characterized by global proteome analysis. The cell cultures established from partal donors differ significantly from those from non-pregnant donors, which is in accordance with in vivo findings. A decrease in alpha-smooth actin and prolyl-4hydroxylase and an increase in interleukin (IL)-6, IL-8 and matrix metalloproteinases (MMP)-1 and MMP-3 were observed in cultures from partal donors. 2D-gel electrophoresis followed by mass spectrometry showed that the expression of 59 proteins was changed significantly in cultures of partal donors. The regulated proteins are involved in protein kinase C signalling, Ca2+ binding, cytoskeletal organization, angiogenesis and degradation. Our data suggest that remodelling of the human cervix is orchestrated by fibroblasts, which are activated or recruited by the inflammatory processes occurring during the ripening cascade.},
  author       = {Malmström, Erik and Sennstrom, Maria and Holmberg, Anna and Frielingsdorf, Helena and Eklund, Erik and Malmstrom, Lars and Tufvesson, Ellen and Gomez, Maria and Westergren-Thorsson, Gunilla and Ekman-Ordeberg, Gunvor and Malmstrom, Anders},
  issn         = {1460-2407},
  language     = {eng},
  pages        = {333--341},
  publisher    = {Oxford University Press},
  series       = {Molecular Human Reproduction},
  title        = {The importance of fibroblasts in remodelling of the human uterine cervix during pregnancy and parturition.},
  url          = {http://dx.doi.org/10.1093/molehr/gal117},
  volume       = {13},
  year         = {2007},
}