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Differently charged polypeptides in the prevention of post-surgical peritoneal adhesions.

Nehéz, Laszlo LU ; Tingstedt, Bobby LU ; Axelsson, Jakob B LU and Andersson, Roland LU (2007) In Scandinavian Journal of Gastroenterology 42(4). p.519-523
Abstract
Objective. Peritoneal adhesions develop after almost all surgical interventions in the abdomen. We have developed an efficient treatment against post-surgical adhesions consisting of a combination of positively charged poly-L-lysine and negatively charged poly-L-glutamate. The aim of the present study was to further develop the concept of applying oppositely charged polypeptides in the prevention of adhesion formation, by evaluating different doses of the peptides, alterations in the way of administration, and also testing alternative components. Material and methods. Eighty-five NMRI mice were divided into six groups. A standardized peritoneal injury model was used. The groups received physiologic sodium chlorine, poly-L-lysine +... (More)
Objective. Peritoneal adhesions develop after almost all surgical interventions in the abdomen. We have developed an efficient treatment against post-surgical adhesions consisting of a combination of positively charged poly-L-lysine and negatively charged poly-L-glutamate. The aim of the present study was to further develop the concept of applying oppositely charged polypeptides in the prevention of adhesion formation, by evaluating different doses of the peptides, alterations in the way of administration, and also testing alternative components. Material and methods. Eighty-five NMRI mice were divided into six groups. A standardized peritoneal injury model was used. The groups received physiologic sodium chlorine, poly-L-lysine + poly-L-glutamate, low molecular weight poly-L-lysine + poly-L-glutamate, locally administered poly-L-lysine + poly-L-glutamate, in vitro mixed poly-L-lysine + poly-L-glutamate and poly-L-arginine + poly-L-glutamate, respectively. After 7 days, the extent of adhesion formation was determined during relaparotomy and was expressed as the mean percentage of the total wound length. Results. A significant decrease (p < 0.001) in the peritoneal adhesion rate was detected in all groups, with the exception of the group administered poly-L-arginine. Among those animals that received poly-L-lysine and poly-L-glutamate, the low dose of poly-L-lysine administration resulted in the most pronounced anti-adhesive effect. Conclusions. The most effective polypeptide combination was poly-L-lysine and poly-L-glutamate, also showing effectiveness when used at low doses and by local application. The differences in adhesion prevention and the possible underlying mechanisms are discussed and the key role of poly-L-lysine is elucidated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
poly-L-lysine, prevention, peritoneal adhesions, poly-L-glutamate
in
Scandinavian Journal of Gastroenterology
volume
42
issue
4
pages
519 - 523
publisher
Taylor & Francis
external identifiers
  • wos:000245629200015
  • scopus:34147171598
ISSN
1502-7708
DOI
10.1080/00365520600988204
language
English
LU publication?
yes
id
0caf9904-0739-4d75-ae76-a740f699d1ea (old id 167398)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17454864&dopt=Abstract
date added to LUP
2007-07-23 16:15:56
date last changed
2017-01-01 06:44:17
@article{0caf9904-0739-4d75-ae76-a740f699d1ea,
  abstract     = {Objective. Peritoneal adhesions develop after almost all surgical interventions in the abdomen. We have developed an efficient treatment against post-surgical adhesions consisting of a combination of positively charged poly-L-lysine and negatively charged poly-L-glutamate. The aim of the present study was to further develop the concept of applying oppositely charged polypeptides in the prevention of adhesion formation, by evaluating different doses of the peptides, alterations in the way of administration, and also testing alternative components. Material and methods. Eighty-five NMRI mice were divided into six groups. A standardized peritoneal injury model was used. The groups received physiologic sodium chlorine, poly-L-lysine + poly-L-glutamate, low molecular weight poly-L-lysine + poly-L-glutamate, locally administered poly-L-lysine + poly-L-glutamate, in vitro mixed poly-L-lysine + poly-L-glutamate and poly-L-arginine + poly-L-glutamate, respectively. After 7 days, the extent of adhesion formation was determined during relaparotomy and was expressed as the mean percentage of the total wound length. Results. A significant decrease (p &lt; 0.001) in the peritoneal adhesion rate was detected in all groups, with the exception of the group administered poly-L-arginine. Among those animals that received poly-L-lysine and poly-L-glutamate, the low dose of poly-L-lysine administration resulted in the most pronounced anti-adhesive effect. Conclusions. The most effective polypeptide combination was poly-L-lysine and poly-L-glutamate, also showing effectiveness when used at low doses and by local application. The differences in adhesion prevention and the possible underlying mechanisms are discussed and the key role of poly-L-lysine is elucidated.},
  author       = {Nehéz, Laszlo and Tingstedt, Bobby and Axelsson, Jakob B and Andersson, Roland},
  issn         = {1502-7708},
  keyword      = {poly-L-lysine,prevention,peritoneal adhesions,poly-L-glutamate},
  language     = {eng},
  number       = {4},
  pages        = {519--523},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Differently charged polypeptides in the prevention of post-surgical peritoneal adhesions.},
  url          = {http://dx.doi.org/10.1080/00365520600988204},
  volume       = {42},
  year         = {2007},
}