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Distinct signalling pathways and transcriptome response signatures differentiate ammonium- and nitrate-supplied plants

Patterson, Kurt; Cakmak, Turgay; Cooper, Andrew; Lager, Ida LU ; Rasmusson, Allan LU and Escobar, Matthew A. (2010) In Plant, Cell and Environment 33(9). p.1486-1501
Abstract
Nitrogen is the only macronutrient that is commonly available to plants in both oxidized and reduced forms, mainly nitrate and ammonium. The physiological and molecular effects of nitrate supply have been well studied, but comparatively little is known about ammonium nutrition and its differential effects on cell function and gene expression. We have used a physiologically realistic hydroponic growth system to compare the transcriptomes and redox status of the roots of ammonium- and nitrate-supplied Arabidopsis thaliana plants. While similar to 60% of nitrogen-regulated genes displayed common responses to both ammonium and nitrate, significant 'nitrate-specific' and 'ammonium-specific' gene sets were identified. Pathways involved in... (More)
Nitrogen is the only macronutrient that is commonly available to plants in both oxidized and reduced forms, mainly nitrate and ammonium. The physiological and molecular effects of nitrate supply have been well studied, but comparatively little is known about ammonium nutrition and its differential effects on cell function and gene expression. We have used a physiologically realistic hydroponic growth system to compare the transcriptomes and redox status of the roots of ammonium- and nitrate-supplied Arabidopsis thaliana plants. While similar to 60% of nitrogen-regulated genes displayed common responses to both ammonium and nitrate, significant 'nitrate-specific' and 'ammonium-specific' gene sets were identified. Pathways involved in cytokinin response and reductant generation/distribution were specifically altered by nitrate, while a complex biotic stress response and changes in nodulin gene expression were characteristic of ammonium-supplied plants. Nitrate supply was associated with a rapid decrease in H2O2 production, potentially because of an increased export of reductant from the mitochondrial matrix. The underlying basis of the nitrate- and ammonium-specific patterns of gene expression appears to be different signals elaborated from each nitrogen source, including alterations in extracellular pH that are associated with ammonium uptake, downstream metabolites in the ammonium assimilation pathway, and the presence or absence of the nitrate ion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
species, reactive oxygen, nitrogen, nitrate, ammonium, Arabidopsis thaliana, redox balancing
in
Plant, Cell and Environment
volume
33
issue
9
pages
1486 - 1501
publisher
Wiley-Blackwell
external identifiers
  • wos:000280661300005
  • scopus:77955238011
ISSN
0140-7791
DOI
10.1111/j.1365-3040.2010.02158.x
language
English
LU publication?
yes
id
f1c11a10-eca1-42a6-bf8c-d8d4f3d2e352 (old id 1674148)
date added to LUP
2010-09-22 13:56:48
date last changed
2018-07-15 03:08:35
@article{f1c11a10-eca1-42a6-bf8c-d8d4f3d2e352,
  abstract     = {Nitrogen is the only macronutrient that is commonly available to plants in both oxidized and reduced forms, mainly nitrate and ammonium. The physiological and molecular effects of nitrate supply have been well studied, but comparatively little is known about ammonium nutrition and its differential effects on cell function and gene expression. We have used a physiologically realistic hydroponic growth system to compare the transcriptomes and redox status of the roots of ammonium- and nitrate-supplied Arabidopsis thaliana plants. While similar to 60% of nitrogen-regulated genes displayed common responses to both ammonium and nitrate, significant 'nitrate-specific' and 'ammonium-specific' gene sets were identified. Pathways involved in cytokinin response and reductant generation/distribution were specifically altered by nitrate, while a complex biotic stress response and changes in nodulin gene expression were characteristic of ammonium-supplied plants. Nitrate supply was associated with a rapid decrease in H2O2 production, potentially because of an increased export of reductant from the mitochondrial matrix. The underlying basis of the nitrate- and ammonium-specific patterns of gene expression appears to be different signals elaborated from each nitrogen source, including alterations in extracellular pH that are associated with ammonium uptake, downstream metabolites in the ammonium assimilation pathway, and the presence or absence of the nitrate ion.},
  author       = {Patterson, Kurt and Cakmak, Turgay and Cooper, Andrew and Lager, Ida and Rasmusson, Allan and Escobar, Matthew A.},
  issn         = {0140-7791},
  keyword      = {species,reactive oxygen,nitrogen,nitrate,ammonium,Arabidopsis thaliana,redox balancing},
  language     = {eng},
  number       = {9},
  pages        = {1486--1501},
  publisher    = {Wiley-Blackwell},
  series       = {Plant, Cell and Environment},
  title        = {Distinct signalling pathways and transcriptome response signatures differentiate ammonium- and nitrate-supplied plants},
  url          = {http://dx.doi.org/10.1111/j.1365-3040.2010.02158.x},
  volume       = {33},
  year         = {2010},
}