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Characterization of the IgD binding site of encapsulated Haemophilus influenzae serotype b.

Samuelsson, Martin LU ; Hallström, Teresia LU ; Forsgren, Arne LU and Riesbeck, Kristian LU (2007) In Journal of Immunology 178(10). p.6316-6319
Abstract
Encapsulated Haemophilus influenzae is a causative agent of invasive disease, such as meningitis and septicemia. Several interactions exist between H. influenzae and the human host. H. influenzae has been reported to bind IgD in a nonimmune manner, but the responsible protein has not yet been identified. To define the binding site on IgD for H. influenzae, full-length IgD and four chimeric IgDs with interspersed TgG sequences and Ag specificity for dansyl chloride were expressed in stably transfected Chinese hamster ovary cells. The binding of recombinant IgD to a panel of encapsulated H. influenzae serotype b (Hib) and nontypeable strains were investigated using a whole cell ELISA and flow cytometry. IgD binding was detected in 50% of the... (More)
Encapsulated Haemophilus influenzae is a causative agent of invasive disease, such as meningitis and septicemia. Several interactions exist between H. influenzae and the human host. H. influenzae has been reported to bind IgD in a nonimmune manner, but the responsible protein has not yet been identified. To define the binding site on IgD for H. influenzae, full-length IgD and four chimeric IgDs with interspersed TgG sequences and Ag specificity for dansyl chloride were expressed in stably transfected Chinese hamster ovary cells. The binding of recombinant IgD to a panel of encapsulated H. influenzae serotype b (Hib) and nontypeable strains were investigated using a whole cell ELISA and flow cytometry. IgD binding was detected in 50% of the encapsulated Hib strains examined, whereas nontypeable H. influenzae did not interact with IgD. Finally, mapping experiments using the chimeric IgD/IgG indicated that IgD C(H)1 aa 198-224 were involved in the interaction between IgD and H. influenzae. Thus, by using recombinant IgD and chimeras with defined Ag specificity, we have confirmed that Hib specifically binds IgD, and that this binding involves the IgD CHI region. (Less)
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organization
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Contribution to journal
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published
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in
Journal of Immunology
volume
178
issue
10
pages
6316 - 6319
publisher
American Association of Immunologists
external identifiers
  • wos:000246286200038
  • scopus:34248158196
ISSN
1550-6606
language
English
LU publication?
yes
id
229b5d76-f3ce-4f7c-a070-beae69f04864 (old id 168461)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17475860&dopt=Abstract
date added to LUP
2007-07-27 08:29:45
date last changed
2017-01-01 06:59:35
@article{229b5d76-f3ce-4f7c-a070-beae69f04864,
  abstract     = {Encapsulated Haemophilus influenzae is a causative agent of invasive disease, such as meningitis and septicemia. Several interactions exist between H. influenzae and the human host. H. influenzae has been reported to bind IgD in a nonimmune manner, but the responsible protein has not yet been identified. To define the binding site on IgD for H. influenzae, full-length IgD and four chimeric IgDs with interspersed TgG sequences and Ag specificity for dansyl chloride were expressed in stably transfected Chinese hamster ovary cells. The binding of recombinant IgD to a panel of encapsulated H. influenzae serotype b (Hib) and nontypeable strains were investigated using a whole cell ELISA and flow cytometry. IgD binding was detected in 50% of the encapsulated Hib strains examined, whereas nontypeable H. influenzae did not interact with IgD. Finally, mapping experiments using the chimeric IgD/IgG indicated that IgD C(H)1 aa 198-224 were involved in the interaction between IgD and H. influenzae. Thus, by using recombinant IgD and chimeras with defined Ag specificity, we have confirmed that Hib specifically binds IgD, and that this binding involves the IgD CHI region.},
  author       = {Samuelsson, Martin and Hallström, Teresia and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1550-6606},
  language     = {eng},
  number       = {10},
  pages        = {6316--6319},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Characterization of the IgD binding site of encapsulated Haemophilus influenzae serotype b.},
  volume       = {178},
  year         = {2007},
}