Haemophilus influenzae Survival during Complement-Mediated Attacks Is Promoted by Moraxella catarrhalis Outer Membrane Vesicles.
Tan, Thuan Tong LU ; Mörgelin, Matthias LU ; Forsgren, Arne LU and Riesbeck, Kristian LU
(2007)
In Journal of Infectious Diseases
195(11).
p.1661-1670
- Abstract
- Moraxella catarrhalis causes respiratory tract infections in children and in adults with chronic obstructive pulmonary disease. It is often isolated as a copathogen with Haemophilus influenzae. The underlying mechanism for this cohabitation is unclear. Here, in clinical specimens from a patient with M. catarrhalis infection, we document that outer membrane vesicles (OMVs) carrying ubiquitous surface protein (Usp) A1 and UspA2 (hereafter, UspA1/A2) were secreted. Further analyses revealed that OMVs isolated in vitro also contained UspA1/A2, which mediate interactions with, among other proteins, the third component of the complement system (C3). OMVs from M. catarrhalis wild-type clinical strains bound to C3 and counteracted the complement... (More)
- Moraxella catarrhalis causes respiratory tract infections in children and in adults with chronic obstructive pulmonary disease. It is often isolated as a copathogen with Haemophilus influenzae. The underlying mechanism for this cohabitation is unclear. Here, in clinical specimens from a patient with M. catarrhalis infection, we document that outer membrane vesicles (OMVs) carrying ubiquitous surface protein (Usp) A1 and UspA2 (hereafter, UspA1/A2) were secreted. Further analyses revealed that OMVs isolated in vitro also contained UspA1/A2, which mediate interactions with, among other proteins, the third component of the complement system (C3). OMVs from M. catarrhalis wild-type clinical strains bound to C3 and counteracted the complement cascade to a larger extent than did OMVs without UspA1/A2. In contrast, UspA1/A2-deficient OMVs were significantly weaker inhibitors of complement-dependent killing of H. influenzae. Thus, our results suggest that a novel strategy exists in which pathogens collaborate to conquer innate immunity and that the M. catarrhalis vaccine candidates UspA1/A2 play a major role in this interaction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/168498
- author
- Tan, Thuan Tong
LU
; Mörgelin, Matthias
LU
; Forsgren, Arne
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 195
- issue
- 11
- pages
- 1661 - 1670
- publisher
- Oxford University Press
- external identifiers
-
- wos:000246200700015
- scopus:34249089830
- pmid:17471436
- ISSN
- 1537-6613
- DOI
- 10.1086/517611
- language
- English
- LU publication?
- yes
- id
- dda309ac-831f-4dcf-8da3-776f483d578b (old id 168498)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17471436&dopt=Abstract
- date added to LUP
- 2016-04-01 17:15:49
- date last changed
- 2022-05-16 19:09:57
@article{dda309ac-831f-4dcf-8da3-776f483d578b,
abstract = {{Moraxella catarrhalis causes respiratory tract infections in children and in adults with chronic obstructive pulmonary disease. It is often isolated as a copathogen with Haemophilus influenzae. The underlying mechanism for this cohabitation is unclear. Here, in clinical specimens from a patient with M. catarrhalis infection, we document that outer membrane vesicles (OMVs) carrying ubiquitous surface protein (Usp) A1 and UspA2 (hereafter, UspA1/A2) were secreted. Further analyses revealed that OMVs isolated in vitro also contained UspA1/A2, which mediate interactions with, among other proteins, the third component of the complement system (C3). OMVs from M. catarrhalis wild-type clinical strains bound to C3 and counteracted the complement cascade to a larger extent than did OMVs without UspA1/A2. In contrast, UspA1/A2-deficient OMVs were significantly weaker inhibitors of complement-dependent killing of H. influenzae. Thus, our results suggest that a novel strategy exists in which pathogens collaborate to conquer innate immunity and that the M. catarrhalis vaccine candidates UspA1/A2 play a major role in this interaction.}},
author = {{Tan, Thuan Tong and Mörgelin, Matthias and Forsgren, Arne and Riesbeck, Kristian}},
issn = {{1537-6613}},
language = {{eng}},
number = {{11}},
pages = {{1661--1670}},
publisher = {{Oxford University Press}},
series = {{Journal of Infectious Diseases}},
title = {{Haemophilus influenzae Survival during Complement-Mediated Attacks Is Promoted by Moraxella catarrhalis Outer Membrane Vesicles.}},
url = {{http://dx.doi.org/10.1086/517611}},
doi = {{10.1086/517611}},
volume = {{195}},
year = {{2007}},
}