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Haemophilus influenzae Survival during Complement-Mediated Attacks Is Promoted by Moraxella catarrhalis Outer Membrane Vesicles.

Tan, Thuan Tong LU ; Mörgelin, Matthias LU ; Forsgren, Arne LU and Riesbeck, Kristian LU (2007) In Journal of Infectious Diseases 195(11). p.1661-1670
Abstract
Moraxella catarrhalis causes respiratory tract infections in children and in adults with chronic obstructive pulmonary disease. It is often isolated as a copathogen with Haemophilus influenzae. The underlying mechanism for this cohabitation is unclear. Here, in clinical specimens from a patient with M. catarrhalis infection, we document that outer membrane vesicles (OMVs) carrying ubiquitous surface protein (Usp) A1 and UspA2 (hereafter, UspA1/A2) were secreted. Further analyses revealed that OMVs isolated in vitro also contained UspA1/A2, which mediate interactions with, among other proteins, the third component of the complement system (C3). OMVs from M. catarrhalis wild-type clinical strains bound to C3 and counteracted the complement... (More)
Moraxella catarrhalis causes respiratory tract infections in children and in adults with chronic obstructive pulmonary disease. It is often isolated as a copathogen with Haemophilus influenzae. The underlying mechanism for this cohabitation is unclear. Here, in clinical specimens from a patient with M. catarrhalis infection, we document that outer membrane vesicles (OMVs) carrying ubiquitous surface protein (Usp) A1 and UspA2 (hereafter, UspA1/A2) were secreted. Further analyses revealed that OMVs isolated in vitro also contained UspA1/A2, which mediate interactions with, among other proteins, the third component of the complement system (C3). OMVs from M. catarrhalis wild-type clinical strains bound to C3 and counteracted the complement cascade to a larger extent than did OMVs without UspA1/A2. In contrast, UspA1/A2-deficient OMVs were significantly weaker inhibitors of complement-dependent killing of H. influenzae. Thus, our results suggest that a novel strategy exists in which pathogens collaborate to conquer innate immunity and that the M. catarrhalis vaccine candidates UspA1/A2 play a major role in this interaction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
195
issue
11
pages
1661 - 1670
publisher
Oxford University Press
external identifiers
  • wos:000246200700015
  • scopus:34249089830
ISSN
1537-6613
DOI
10.1086/517611
language
English
LU publication?
yes
id
dda309ac-831f-4dcf-8da3-776f483d578b (old id 168498)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17471436&dopt=Abstract
date added to LUP
2007-07-27 11:18:36
date last changed
2017-11-12 04:05:35
@article{dda309ac-831f-4dcf-8da3-776f483d578b,
  abstract     = {Moraxella catarrhalis causes respiratory tract infections in children and in adults with chronic obstructive pulmonary disease. It is often isolated as a copathogen with Haemophilus influenzae. The underlying mechanism for this cohabitation is unclear. Here, in clinical specimens from a patient with M. catarrhalis infection, we document that outer membrane vesicles (OMVs) carrying ubiquitous surface protein (Usp) A1 and UspA2 (hereafter, UspA1/A2) were secreted. Further analyses revealed that OMVs isolated in vitro also contained UspA1/A2, which mediate interactions with, among other proteins, the third component of the complement system (C3). OMVs from M. catarrhalis wild-type clinical strains bound to C3 and counteracted the complement cascade to a larger extent than did OMVs without UspA1/A2. In contrast, UspA1/A2-deficient OMVs were significantly weaker inhibitors of complement-dependent killing of H. influenzae. Thus, our results suggest that a novel strategy exists in which pathogens collaborate to conquer innate immunity and that the M. catarrhalis vaccine candidates UspA1/A2 play a major role in this interaction.},
  author       = {Tan, Thuan Tong and Mörgelin, Matthias and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1537-6613},
  language     = {eng},
  number       = {11},
  pages        = {1661--1670},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Haemophilus influenzae Survival during Complement-Mediated Attacks Is Promoted by Moraxella catarrhalis Outer Membrane Vesicles.},
  url          = {http://dx.doi.org/10.1086/517611},
  volume       = {195},
  year         = {2007},
}