Expression of TGF-beta isoforms, TGF-beta receptors, and SMAD molecules at different stages of human glioma
(2000) In International Journal of Cancer 89(3). p.251-258- Abstract
- Human gliomas express TGF-beta but, so far the expression of downstream mediators has been investigated in only a few cell lines. We have examined tissue specimens of 23 gliomas: 3 astrocytomas grade II (AST), 8 anaplastic astrocytomas grade III (AAST), and 12 glioblastoma multiforme grade IV (GBM). We analyzed the mRNA expression of TGF-beta1, TGF-beta2, TGF-beta3, the TGF-beta receptors type I (TbetaR-I) and type II (TbetaR-II), Smad2, Smad3, and Smad4. mRNA expression of IL-10 and CD95 (FAS/APO-1) were also studied. We detected increased mRNA levels of the 3 TGF-beta isoforms, correlating with the degree of malignancy. TGF-beta3 mRNA was increased, particularly in AST and AAST, while TGF-beta1 and TGF-beta2 mRNAs were strongly expressed... (More)
- Human gliomas express TGF-beta but, so far the expression of downstream mediators has been investigated in only a few cell lines. We have examined tissue specimens of 23 gliomas: 3 astrocytomas grade II (AST), 8 anaplastic astrocytomas grade III (AAST), and 12 glioblastoma multiforme grade IV (GBM). We analyzed the mRNA expression of TGF-beta1, TGF-beta2, TGF-beta3, the TGF-beta receptors type I (TbetaR-I) and type II (TbetaR-II), Smad2, Smad3, and Smad4. mRNA expression of IL-10 and CD95 (FAS/APO-1) were also studied. We detected increased mRNA levels of the 3 TGF-beta isoforms, correlating with the degree of malignancy. TGF-beta3 mRNA was increased, particularly in AST and AAST, while TGF-beta1 and TGF-beta2 mRNAs were strongly expressed in GBM. TGF-beta normally up-regulates the TGF-beta receptors, and TbetaR-I and TbetaR-II showed stronger expression in all gliomas when compared to normal tissues. However, the mRNA expression of Smad2, Smad3, and Smad4 was decreased in GBM. IL-10 mRNA expression was detected in glioma tissues but not in glioma cell lines. No marked increase in the expression of soluble CD95 splicing variants was found in the gliomas compared with normal tissue. However, total CD95 mRNA was elevated among GBM tissues. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1714543
- author
- Kjellman, Christian LU ; Olofsson, SP ; Hansson, O ; von Schantz, Torbjörn LU ; Lindvall, Magnus LU ; Nilsson, Ingar LU ; Salford, Leif LU ; Sjögren, Hans Olov LU and Widegren, Bengt LU
- organization
-
- Department of Experimental Medical Science
- MEMEG
- Child and Adolescent Psychiatry
- Celiac Disease and Diabetes Unit (research group)
- Stem Cell Center
- Developmental and Regenerative Neurobiology (research group)
- Molecular Neurogenetics (research group)
- Neurosurgery
- Molecular Ecology and Evolution Lab (research group)
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Cancer
- volume
- 89
- issue
- 3
- pages
- 251 - 258
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0033934907
- ISSN
- 0020-7136
- DOI
- 10.1002/1097-0215(20000520)89:3<251::AID-IJC7>3.0.CO;2-5
- language
- English
- LU publication?
- yes
- id
- a47ae990-e39b-47e2-946f-54d6ef4ae1d6 (old id 1714543)
- date added to LUP
- 2016-04-01 11:41:40
- date last changed
- 2024-04-22 12:28:26
@article{a47ae990-e39b-47e2-946f-54d6ef4ae1d6,
abstract = {{Human gliomas express TGF-beta but, so far the expression of downstream mediators has been investigated in only a few cell lines. We have examined tissue specimens of 23 gliomas: 3 astrocytomas grade II (AST), 8 anaplastic astrocytomas grade III (AAST), and 12 glioblastoma multiforme grade IV (GBM). We analyzed the mRNA expression of TGF-beta1, TGF-beta2, TGF-beta3, the TGF-beta receptors type I (TbetaR-I) and type II (TbetaR-II), Smad2, Smad3, and Smad4. mRNA expression of IL-10 and CD95 (FAS/APO-1) were also studied. We detected increased mRNA levels of the 3 TGF-beta isoforms, correlating with the degree of malignancy. TGF-beta3 mRNA was increased, particularly in AST and AAST, while TGF-beta1 and TGF-beta2 mRNAs were strongly expressed in GBM. TGF-beta normally up-regulates the TGF-beta receptors, and TbetaR-I and TbetaR-II showed stronger expression in all gliomas when compared to normal tissues. However, the mRNA expression of Smad2, Smad3, and Smad4 was decreased in GBM. IL-10 mRNA expression was detected in glioma tissues but not in glioma cell lines. No marked increase in the expression of soluble CD95 splicing variants was found in the gliomas compared with normal tissue. However, total CD95 mRNA was elevated among GBM tissues.}},
author = {{Kjellman, Christian and Olofsson, SP and Hansson, O and von Schantz, Torbjörn and Lindvall, Magnus and Nilsson, Ingar and Salford, Leif and Sjögren, Hans Olov and Widegren, Bengt}},
issn = {{0020-7136}},
language = {{eng}},
number = {{3}},
pages = {{251--258}},
publisher = {{John Wiley & Sons Inc.}},
series = {{International Journal of Cancer}},
title = {{Expression of TGF-beta isoforms, TGF-beta receptors, and SMAD molecules at different stages of human glioma}},
url = {{http://dx.doi.org/10.1002/1097-0215(20000520)89:3<251::AID-IJC7>3.0.CO;2-5}},
doi = {{10.1002/1097-0215(20000520)89:3<251::AID-IJC7>3.0.CO;2-5}},
volume = {{89}},
year = {{2000}},
}