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Expression of TGF-beta isoforms, TGF-beta receptors, and SMAD molecules at different stages of human glioma

Kjellman, Christian LU ; Olofsson, SP; Hansson, O; von Schantz, Torbjörn LU ; Lindvall, Magnus LU ; Nilsson, Ingar LU ; Salford, Leif LU ; Sjögren, Hans Olov LU and Widegren, Bengt LU (2000) In International Journal of Cancer 89(3). p.251-258
Abstract
Human gliomas express TGF-beta but, so far the expression of downstream mediators has been investigated in only a few cell lines. We have examined tissue specimens of 23 gliomas: 3 astrocytomas grade II (AST), 8 anaplastic astrocytomas grade III (AAST), and 12 glioblastoma multiforme grade IV (GBM). We analyzed the mRNA expression of TGF-beta1, TGF-beta2, TGF-beta3, the TGF-beta receptors type I (TbetaR-I) and type II (TbetaR-II), Smad2, Smad3, and Smad4. mRNA expression of IL-10 and CD95 (FAS/APO-1) were also studied. We detected increased mRNA levels of the 3 TGF-beta isoforms, correlating with the degree of malignancy. TGF-beta3 mRNA was increased, particularly in AST and AAST, while TGF-beta1 and TGF-beta2 mRNAs were strongly expressed... (More)
Human gliomas express TGF-beta but, so far the expression of downstream mediators has been investigated in only a few cell lines. We have examined tissue specimens of 23 gliomas: 3 astrocytomas grade II (AST), 8 anaplastic astrocytomas grade III (AAST), and 12 glioblastoma multiforme grade IV (GBM). We analyzed the mRNA expression of TGF-beta1, TGF-beta2, TGF-beta3, the TGF-beta receptors type I (TbetaR-I) and type II (TbetaR-II), Smad2, Smad3, and Smad4. mRNA expression of IL-10 and CD95 (FAS/APO-1) were also studied. We detected increased mRNA levels of the 3 TGF-beta isoforms, correlating with the degree of malignancy. TGF-beta3 mRNA was increased, particularly in AST and AAST, while TGF-beta1 and TGF-beta2 mRNAs were strongly expressed in GBM. TGF-beta normally up-regulates the TGF-beta receptors, and TbetaR-I and TbetaR-II showed stronger expression in all gliomas when compared to normal tissues. However, the mRNA expression of Smad2, Smad3, and Smad4 was decreased in GBM. IL-10 mRNA expression was detected in glioma tissues but not in glioma cell lines. No marked increase in the expression of soluble CD95 splicing variants was found in the gliomas compared with normal tissue. However, total CD95 mRNA was elevated among GBM tissues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Cancer
volume
89
issue
3
pages
251 - 258
publisher
John Wiley & Sons
external identifiers
  • scopus:0033934907
ISSN
0020-7136
DOI
10.1002/1097-0215(20000520)89:3<251::AID-IJC7>3.0.CO;2-5
language
English
LU publication?
yes
id
a47ae990-e39b-47e2-946f-54d6ef4ae1d6 (old id 1714543)
date added to LUP
2011-06-22 12:30:51
date last changed
2017-10-29 03:26:38
@article{a47ae990-e39b-47e2-946f-54d6ef4ae1d6,
  abstract     = {Human gliomas express TGF-beta but, so far the expression of downstream mediators has been investigated in only a few cell lines. We have examined tissue specimens of 23 gliomas: 3 astrocytomas grade II (AST), 8 anaplastic astrocytomas grade III (AAST), and 12 glioblastoma multiforme grade IV (GBM). We analyzed the mRNA expression of TGF-beta1, TGF-beta2, TGF-beta3, the TGF-beta receptors type I (TbetaR-I) and type II (TbetaR-II), Smad2, Smad3, and Smad4. mRNA expression of IL-10 and CD95 (FAS/APO-1) were also studied. We detected increased mRNA levels of the 3 TGF-beta isoforms, correlating with the degree of malignancy. TGF-beta3 mRNA was increased, particularly in AST and AAST, while TGF-beta1 and TGF-beta2 mRNAs were strongly expressed in GBM. TGF-beta normally up-regulates the TGF-beta receptors, and TbetaR-I and TbetaR-II showed stronger expression in all gliomas when compared to normal tissues. However, the mRNA expression of Smad2, Smad3, and Smad4 was decreased in GBM. IL-10 mRNA expression was detected in glioma tissues but not in glioma cell lines. No marked increase in the expression of soluble CD95 splicing variants was found in the gliomas compared with normal tissue. However, total CD95 mRNA was elevated among GBM tissues.},
  author       = {Kjellman, Christian and Olofsson, SP and Hansson, O and von Schantz, Torbjörn and Lindvall, Magnus and Nilsson, Ingar and Salford, Leif and Sjögren, Hans Olov and Widegren, Bengt},
  issn         = {0020-7136},
  language     = {eng},
  number       = {3},
  pages        = {251--258},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Expression of TGF-beta isoforms, TGF-beta receptors, and SMAD molecules at different stages of human glioma},
  url          = {http://dx.doi.org/10.1002/1097-0215(20000520)89:3<251::AID-IJC7>3.0.CO;2-5},
  volume       = {89},
  year         = {2000},
}