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Steam pretreatment and fermentation of the straw material "Paja Brava" using simultaneous saccharification and co-fermentation.

Carrasco, Cristhian LU ; Baudel, Henrique; Penarrieta, Mauricio LU ; Solano, Carlos LU ; Tejeda, Leslie LU ; Roslander, Christian LU ; Galbe, Mats LU and Lidén, Gunnar LU (2011) In Journal of Bioscience and Bioengineering 111. p.167-174
Abstract
Pretreatment, enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) of the South American straw material Paja Brava were investigated. Suitable process conditions for an SO(2)-catalyzed steam pretreatment of the material were determined and assessed by enzymatic digestibility of obtained fiber slurries for 72h at a water insoluble solids (WIS) content of 2%. The best pretreatment conditions obtained (200°C, 5min holding time and 2.5% SO(2)) gave an overall glucose yield following enzymatic hydrolysis of more than 90%, and a xylose yield of about 70%. Simultaneous saccharification and co-fermentation of glucose and xylose (SSCF) of the pretreated material using the xylose-fermenting strain Saccharomyces cerevisiae... (More)
Pretreatment, enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) of the South American straw material Paja Brava were investigated. Suitable process conditions for an SO(2)-catalyzed steam pretreatment of the material were determined and assessed by enzymatic digestibility of obtained fiber slurries for 72h at a water insoluble solids (WIS) content of 2%. The best pretreatment conditions obtained (200°C, 5min holding time and 2.5% SO(2)) gave an overall glucose yield following enzymatic hydrolysis of more than 90%, and a xylose yield of about 70%. Simultaneous saccharification and co-fermentation of glucose and xylose (SSCF) of the pretreated material using the xylose-fermenting strain Saccharomyces cerevisiae TMB3400 was examined at WIS contents between 5% and 10%. In agreement with previous studies on other materials, the overall ethanol yield and also the xylose conversion decreased somewhat with increasing WIS content in the SSCF. In batch SSCF, the xylose conversion obtained was almost 100% at 5% WIS content, but decreased to 69% at 10% WIS. The highest ethanol concentration obtained for a WIS content of 10% was about 40g/L, corresponding to a yield of 0.41g/g in a fed-batch SSCF. The Paja Brava material has previously been found difficult to hydrolyze in a dilute-acid process. However, the SSCF results obtained here show that similar sugar yields and fermentation performance can be expected from Paja Brava as from materials such as wheat straw, corn stover or sugarcane bagasse. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bioscience and Bioengineering
volume
111
pages
167 - 174
publisher
Elsevier
external identifiers
  • wos:000288528000010
  • pmid:21081285
  • scopus:78951486947
ISSN
1347-4421
DOI
10.1016/j.jbiosc.2010.10.009
language
English
LU publication?
yes
id
7fcd986d-37dc-424f-b53b-86f0e91fb0ed (old id 1731908)
date added to LUP
2010-12-15 12:36:28
date last changed
2017-11-12 03:00:12
@article{7fcd986d-37dc-424f-b53b-86f0e91fb0ed,
  abstract     = {Pretreatment, enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) of the South American straw material Paja Brava were investigated. Suitable process conditions for an SO(2)-catalyzed steam pretreatment of the material were determined and assessed by enzymatic digestibility of obtained fiber slurries for 72h at a water insoluble solids (WIS) content of 2%. The best pretreatment conditions obtained (200°C, 5min holding time and 2.5% SO(2)) gave an overall glucose yield following enzymatic hydrolysis of more than 90%, and a xylose yield of about 70%. Simultaneous saccharification and co-fermentation of glucose and xylose (SSCF) of the pretreated material using the xylose-fermenting strain Saccharomyces cerevisiae TMB3400 was examined at WIS contents between 5% and 10%. In agreement with previous studies on other materials, the overall ethanol yield and also the xylose conversion decreased somewhat with increasing WIS content in the SSCF. In batch SSCF, the xylose conversion obtained was almost 100% at 5% WIS content, but decreased to 69% at 10% WIS. The highest ethanol concentration obtained for a WIS content of 10% was about 40g/L, corresponding to a yield of 0.41g/g in a fed-batch SSCF. The Paja Brava material has previously been found difficult to hydrolyze in a dilute-acid process. However, the SSCF results obtained here show that similar sugar yields and fermentation performance can be expected from Paja Brava as from materials such as wheat straw, corn stover or sugarcane bagasse.},
  author       = {Carrasco, Cristhian and Baudel, Henrique and Penarrieta, Mauricio and Solano, Carlos and Tejeda, Leslie and Roslander, Christian and Galbe, Mats and Lidén, Gunnar},
  issn         = {1347-4421},
  language     = {eng},
  pages        = {167--174},
  publisher    = {Elsevier},
  series       = {Journal of Bioscience and Bioengineering},
  title        = {Steam pretreatment and fermentation of the straw material "Paja Brava" using simultaneous saccharification and co-fermentation.},
  url          = {http://dx.doi.org/10.1016/j.jbiosc.2010.10.009},
  volume       = {111},
  year         = {2011},
}