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MHC variation in birds and reptiles

Wittzell, Håkan LU ; Madsen, Thomas LU ; Westerdahl, Helena LU ; Shine, R. and von Schantz, Torbjörn LU (1998) In Genetica 104(3). p.301-309
Abstract
The major histocompatibility complex (MHC) has been studied in a multitude of mammals by now, but much less is known about its organisation and variation in other vertebrate species. The mammalian MHC is organised as a single gene cluster, but recent studies on birds suggest that this paradigm of MHC organisation has to be supplemented. The domestic chicken thus possesses two separate gene clusters which both contain MHC class I and class II B genes, and we have shown that the ring-necked pheasant Phasianus colchicus also has two unlinked clusters of class II B genes. We are studying the effect of the MHC on mate choice, survival and reproductive success in natural populations of birds and reptiles. For this reason, we are developing DNA... (More)
The major histocompatibility complex (MHC) has been studied in a multitude of mammals by now, but much less is known about its organisation and variation in other vertebrate species. The mammalian MHC is organised as a single gene cluster, but recent studies on birds suggest that this paradigm of MHC organisation has to be supplemented. The domestic chicken thus possesses two separate gene clusters which both contain MHC class I and class II B genes, and we have shown that the ring-necked pheasant Phasianus colchicus also has two unlinked clusters of class II B genes. We are studying the effect of the MHC on mate choice, survival and reproductive success in natural populations of birds and reptiles. For this reason, we are developing DNA techniques to determine the animals' MHC genotype. The amplification of the hypervariable exon 3 of the class I gene from songbirds and reptiles has provided us with species specific probes that can be used in Southern blot analysis. The first results indicate very extensive variation in all studied species, that is starlings Sturnus vulgaris, great reed warblers Acrocephalus arundinaceus and water pythons Liasis fuscus. The restriction fragment length polymorphism (RFLP) analysis also suggests that the number of MHC genes is significantly larger in these species than in pheasants and domestic chickens. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
reptiles, major histocompatibility complex, class-i molecules, rfp-y, nucleolar, organizer, mating preferences, population biology, genetic variation, chicken mhc, sequences, b-gene, polymorphism, birds, evolution
in
Genetica
volume
104
issue
3
pages
301 - 309
publisher
Kluwer
external identifiers
  • scopus:0032462256
ISSN
1573-6857
project
Avian MHC genes
language
English
LU publication?
yes
id
21381963-d1c2-446b-ab2d-7a189f5af2ce (old id 1747784)
alternative location
http://springerlink.metapress.com/content/p8m3t88610366300/fulltext.pdf
date added to LUP
2011-02-10 14:33:40
date last changed
2017-01-01 06:41:45
@article{21381963-d1c2-446b-ab2d-7a189f5af2ce,
  abstract     = {The major histocompatibility complex (MHC) has been studied in a multitude of mammals by now, but much less is known about its organisation and variation in other vertebrate species. The mammalian MHC is organised as a single gene cluster, but recent studies on birds suggest that this paradigm of MHC organisation has to be supplemented. The domestic chicken thus possesses two separate gene clusters which both contain MHC class I and class II B genes, and we have shown that the ring-necked pheasant Phasianus colchicus also has two unlinked clusters of class II B genes. We are studying the effect of the MHC on mate choice, survival and reproductive success in natural populations of birds and reptiles. For this reason, we are developing DNA techniques to determine the animals' MHC genotype. The amplification of the hypervariable exon 3 of the class I gene from songbirds and reptiles has provided us with species specific probes that can be used in Southern blot analysis. The first results indicate very extensive variation in all studied species, that is starlings Sturnus vulgaris, great reed warblers Acrocephalus arundinaceus and water pythons Liasis fuscus. The restriction fragment length polymorphism (RFLP) analysis also suggests that the number of MHC genes is significantly larger in these species than in pheasants and domestic chickens.},
  author       = {Wittzell, Håkan and Madsen, Thomas and Westerdahl, Helena and Shine, R. and von Schantz, Torbjörn},
  issn         = {1573-6857},
  keyword      = {reptiles,major histocompatibility complex,class-i molecules,rfp-y,nucleolar,organizer,mating preferences,population biology,genetic variation,chicken mhc,sequences,b-gene,polymorphism,birds,evolution},
  language     = {eng},
  number       = {3},
  pages        = {301--309},
  publisher    = {Kluwer},
  series       = {Genetica},
  title        = {MHC variation in birds and reptiles},
  volume       = {104},
  year         = {1998},
}