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Molecular characterization of 3 MHC class II B haplotypes in the ring-necked pheasant

Wittzell, Håkan LU ; von Schantz, Torbjörn LU ; Zoorob, Rima and Auffray, Charles (1994) In Immunogenetics 39(6). p.395-403
Abstract
We investigated the class II B genes in a free-ranging population of the ring-necked pheasant Phasianus colchicus by a combination of restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR), and DNA sequencing. Special attention was paid to the variation in the second exon, which encodes the peptide-binding beta(i)-domain. The population was introduced, but it still exhibited major histocompatibility complex polymorphism with at least three segregating class II B haplotypes and consequently six genotypes. We found two class LI B genes associated with each haplotype. The class II B genes of birds had until then only been molecularly characterized in the domestic chicken. The pheasant genes were highly variable,... (More)
We investigated the class II B genes in a free-ranging population of the ring-necked pheasant Phasianus colchicus by a combination of restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR), and DNA sequencing. Special attention was paid to the variation in the second exon, which encodes the peptide-binding beta(i)-domain. The population was introduced, but it still exhibited major histocompatibility complex polymorphism with at least three segregating class II B haplotypes and consequently six genotypes. We found two class LI B genes associated with each haplotype. The class II B genes of birds had until then only been molecularly characterized in the domestic chicken. The pheasant genes were highly variable, although one of the amplified sequences was found in two different haplotypes. Taken together, the most polymorphic positions (residues 37 and 38) were not identical in any of the predicted protein sequences, but all except one of the motifs had already been found in the domestic chicken. Structurally important features in mammalian class II B genes were generally conserved also in the pheasant sequences, but the loss of a potential salt bridge constituent (Arg(72)) in several sequences may suggest a slightly different structure of the adjacent parts of the peptide-binding groove. The pheasant genes are most closely related to the so called B-LBII family in the chicken, indicating that this represents a major line of development among avian class II B genes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
selection, phasianus-colchicus, major histocompatibility complex, genes, chicken, parasites, chains, sequences, evolution, clones
in
Immunogenetics
volume
39
issue
6
pages
395 - 403
publisher
Springer
external identifiers
  • scopus:0028176353
ISSN
1432-1211
DOI
10.1007/BF00176156
project
Avian MHC genes
language
English
LU publication?
yes
id
9f2cf177-5a89-4457-b38b-e70989634f2e (old id 1747791)
date added to LUP
2011-02-22 12:28:57
date last changed
2017-07-30 04:47:01
@article{9f2cf177-5a89-4457-b38b-e70989634f2e,
  abstract     = {We investigated the class II B genes in a free-ranging population of the ring-necked pheasant Phasianus colchicus by a combination of restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR), and DNA sequencing. Special attention was paid to the variation in the second exon, which encodes the peptide-binding beta(i)-domain. The population was introduced, but it still exhibited major histocompatibility complex polymorphism with at least three segregating class II B haplotypes and consequently six genotypes. We found two class LI B genes associated with each haplotype. The class II B genes of birds had until then only been molecularly characterized in the domestic chicken. The pheasant genes were highly variable, although one of the amplified sequences was found in two different haplotypes. Taken together, the most polymorphic positions (residues 37 and 38) were not identical in any of the predicted protein sequences, but all except one of the motifs had already been found in the domestic chicken. Structurally important features in mammalian class II B genes were generally conserved also in the pheasant sequences, but the loss of a potential salt bridge constituent (Arg(72)) in several sequences may suggest a slightly different structure of the adjacent parts of the peptide-binding groove. The pheasant genes are most closely related to the so called B-LBII family in the chicken, indicating that this represents a major line of development among avian class II B genes.},
  author       = {Wittzell, Håkan and von Schantz, Torbjörn and Zoorob, Rima and Auffray, Charles},
  issn         = {1432-1211},
  keyword      = {selection,phasianus-colchicus,major histocompatibility complex,genes,chicken,parasites,chains,sequences,evolution,clones},
  language     = {eng},
  number       = {6},
  pages        = {395--403},
  publisher    = {Springer},
  series       = {Immunogenetics},
  title        = {Molecular characterization of 3 MHC class II B haplotypes in the ring-necked pheasant},
  url          = {http://dx.doi.org/10.1007/BF00176156},
  volume       = {39},
  year         = {1994},
}