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The MreB-like protein Mbl of S. coelicolor A3(2) depends on MreB for proper localization and contributes to spore wall synthesis.

Heichlinger, Andrea; Ammelburg, Moritz; Kleinschnitz, Eva-Maria; Latus, Annette; Maldener, Iris; Flärdh, Klas LU ; Wohlleben, Wolfgang and Muth, Günther (2011) In Journal of Bacteriology 193(7). p.1533-1542
Abstract
Most bacteria with a rod-shape morphology contain an actin-like cytoskeleton consisting of MreB polymers which form helical spirals underneath the cytoplasmic membrane to direct peptidoglycan synthesis for elongation of the cell wall. In contrast MreB of Streptomyces coelicolor is not required for vegetative growth, but has a role in sporulation. Beside MreB, S. coelicolor encodes two further MreB-like proteins, Mbl and SCO6166, whose function is unknown. Whereas MreB and Mbl are highly similar, SCO6166 is shorter, lacking the subdomains IB and IIB of actin-like proteins. Here we showed that MreB and Mbl are not functionally redundant but cooperate in spore wall synthesis. Expression analysis by semi-quantitative RT-PCR revealed distinct... (More)
Most bacteria with a rod-shape morphology contain an actin-like cytoskeleton consisting of MreB polymers which form helical spirals underneath the cytoplasmic membrane to direct peptidoglycan synthesis for elongation of the cell wall. In contrast MreB of Streptomyces coelicolor is not required for vegetative growth, but has a role in sporulation. Beside MreB, S. coelicolor encodes two further MreB-like proteins, Mbl and SCO6166, whose function is unknown. Whereas MreB and Mbl are highly similar, SCO6166 is shorter, lacking the subdomains IB and IIB of actin-like proteins. Here we showed that MreB and Mbl are not functionally redundant but cooperate in spore wall synthesis. Expression analysis by semi-quantitative RT-PCR revealed distinct expression patterns. mreB and mbl are predominantly induced during morphological differentiation. In contrast sco6166 is strongly expressed during vegetative growth but switched off during sporulation. All genes could be deleted without affecting viability. Even a ΔmreB/mbl double mutant was viable. Δsco6166 had a wildtype phenotype. ΔmreB, Δmbl and ΔmreB/mbl produced swollen prematurely germinating spores that were sensitive to various kinds of stress, suggesting a defect in spore wall integrity. During aerial mycelium formation an Mbl-mCherry fusion protein colocalized with an MreB-eGFP fusion protein at the sporulation septa. Whereas MreB-eGFP localized properly in the Δmbl mutant, Mbl-mCherry localization depended on the presence of a functional MreB protein. Our results revealed that MreB and Mbl cooperate in synthesis of the thickened spore wall, while SCO6166 has a non-essential function during vegetative growth. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytoskeleton, cell wall synthesis, peptidoglycan, actin-like, sporulation
in
Journal of Bacteriology
volume
193
issue
7
pages
1533 - 1542
publisher
American Society for Microbiology
external identifiers
  • wos:000288314400005
  • scopus:79951809110
ISSN
0021-9193
DOI
10.1128/JB.01100-10
language
English
LU publication?
yes
id
546ba9ac-e190-43f1-a867-6abab7792082 (old id 1777111)
date added to LUP
2011-02-07 12:56:06
date last changed
2017-11-19 03:07:16
@article{546ba9ac-e190-43f1-a867-6abab7792082,
  abstract     = {Most bacteria with a rod-shape morphology contain an actin-like cytoskeleton consisting of MreB polymers which form helical spirals underneath the cytoplasmic membrane to direct peptidoglycan synthesis for elongation of the cell wall. In contrast MreB of Streptomyces coelicolor is not required for vegetative growth, but has a role in sporulation. Beside MreB, S. coelicolor encodes two further MreB-like proteins, Mbl and SCO6166, whose function is unknown. Whereas MreB and Mbl are highly similar, SCO6166 is shorter, lacking the subdomains IB and IIB of actin-like proteins. Here we showed that MreB and Mbl are not functionally redundant but cooperate in spore wall synthesis. Expression analysis by semi-quantitative RT-PCR revealed distinct expression patterns. mreB and mbl are predominantly induced during morphological differentiation. In contrast sco6166 is strongly expressed during vegetative growth but switched off during sporulation. All genes could be deleted without affecting viability. Even a ΔmreB/mbl double mutant was viable. Δsco6166 had a wildtype phenotype. ΔmreB, Δmbl and ΔmreB/mbl produced swollen prematurely germinating spores that were sensitive to various kinds of stress, suggesting a defect in spore wall integrity. During aerial mycelium formation an Mbl-mCherry fusion protein colocalized with an MreB-eGFP fusion protein at the sporulation septa. Whereas MreB-eGFP localized properly in the Δmbl mutant, Mbl-mCherry localization depended on the presence of a functional MreB protein. Our results revealed that MreB and Mbl cooperate in synthesis of the thickened spore wall, while SCO6166 has a non-essential function during vegetative growth.},
  author       = {Heichlinger, Andrea and Ammelburg, Moritz and Kleinschnitz, Eva-Maria and Latus, Annette and Maldener, Iris and Flärdh, Klas and Wohlleben, Wolfgang and Muth, Günther},
  issn         = {0021-9193},
  keyword      = {cytoskeleton,cell wall synthesis,peptidoglycan,actin-like,sporulation},
  language     = {eng},
  number       = {7},
  pages        = {1533--1542},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {The MreB-like protein Mbl of S. coelicolor A3(2) depends on MreB for proper localization and contributes to spore wall synthesis.},
  url          = {http://dx.doi.org/10.1128/JB.01100-10},
  volume       = {193},
  year         = {2011},
}