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UV-B-induced DNA damage mediates expression changes of cell cycle regulatory genes in Arabidopsis root tips

Jiang, Lei; Wang, Yan; Björn, Lars Olof LU and Li, Shaoshan (2011) In Planta 233(4). p.831-841
Abstract
Even though a number of studies have shown that UV-B radiation inhibits plant growth and regulates the cell cycle progress, little is known about the molecular and cellular mechanisms. Here, we developed a synchronous root-tip cell system to investigate expression changes of cell cycle marker genes and DNA damage under UV-B radiation. Expression analysis of cell cycle marker genes revealed that G1-to-S transition in root-tip cells was accomplished within 6 h. In the in vivo synchronous root-tip cells, high level of UV-B radiation (0.45 W m(-2)) induced expression changes of the cell cycle regulatory genes. Genes involved in G1-to-S transition, Histone H4 and E2Fa, were down-regulated by UV-B radiation during 2-6 h; whereas transcripts for... (More)
Even though a number of studies have shown that UV-B radiation inhibits plant growth and regulates the cell cycle progress, little is known about the molecular and cellular mechanisms. Here, we developed a synchronous root-tip cell system to investigate expression changes of cell cycle marker genes and DNA damage under UV-B radiation. Expression analysis of cell cycle marker genes revealed that G1-to-S transition in root-tip cells was accomplished within 6 h. In the in vivo synchronous root-tip cells, high level of UV-B radiation (0.45 W m(-2)) induced expression changes of the cell cycle regulatory genes. Genes involved in G1-to-S transition, Histone H4 and E2Fa, were down-regulated by UV-B radiation during 2-6 h; whereas transcripts for KRP2, a negative regulator of G1-to-S transition, were up-regulated by UV-B at 2 h. The peak time for transcript level of CYCD3;1, a positive factor in G1-to-S transition, was delayed by UV-B radiation. Interestingly, a medium level of UV-B radiation (0.25 W m(-2)) did not change the expression of these genes in root tip cells from wild type. However, cell cycle regulatory genes were greatly affected in uvh1 mutant, which exhibited higher content of cyclobutane pyrimidine dimers (CPDs). Ascorbic acid treatment did not change the expression pattern of cell cycle regulatory genes that were affected by high-level UV-B. Our results implied that UV-B-induced DNA damage results in the delay of G1-to-S transition of plant cell cycle. UV-B-induced G1-to-S arrest may be a protective mechanism that prevents cells with damaged DNA from dividing and may explain the plant growth inhibition under increased solar UV-B radiation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Arabidopsis, CDK, CPD, Cyclin D, Cell cycle, G1-to-S transition, UV-B, Root tip
in
Planta
volume
233
issue
4
pages
831 - 841
publisher
Springer
external identifiers
  • wos:000288804000015
  • scopus:79953030640
ISSN
0032-0935
DOI
10.1007/s00425-010-1340-5
project
Photobiology
language
English
LU publication?
yes
id
0ab7820a-dfa7-47eb-bae3-64dc83a6fc97 (old id 1788872)
date added to LUP
2011-07-14 09:25:57
date last changed
2017-10-01 04:07:04
@article{0ab7820a-dfa7-47eb-bae3-64dc83a6fc97,
  abstract     = {Even though a number of studies have shown that UV-B radiation inhibits plant growth and regulates the cell cycle progress, little is known about the molecular and cellular mechanisms. Here, we developed a synchronous root-tip cell system to investigate expression changes of cell cycle marker genes and DNA damage under UV-B radiation. Expression analysis of cell cycle marker genes revealed that G1-to-S transition in root-tip cells was accomplished within 6 h. In the in vivo synchronous root-tip cells, high level of UV-B radiation (0.45 W m(-2)) induced expression changes of the cell cycle regulatory genes. Genes involved in G1-to-S transition, Histone H4 and E2Fa, were down-regulated by UV-B radiation during 2-6 h; whereas transcripts for KRP2, a negative regulator of G1-to-S transition, were up-regulated by UV-B at 2 h. The peak time for transcript level of CYCD3;1, a positive factor in G1-to-S transition, was delayed by UV-B radiation. Interestingly, a medium level of UV-B radiation (0.25 W m(-2)) did not change the expression of these genes in root tip cells from wild type. However, cell cycle regulatory genes were greatly affected in uvh1 mutant, which exhibited higher content of cyclobutane pyrimidine dimers (CPDs). Ascorbic acid treatment did not change the expression pattern of cell cycle regulatory genes that were affected by high-level UV-B. Our results implied that UV-B-induced DNA damage results in the delay of G1-to-S transition of plant cell cycle. UV-B-induced G1-to-S arrest may be a protective mechanism that prevents cells with damaged DNA from dividing and may explain the plant growth inhibition under increased solar UV-B radiation.},
  author       = {Jiang, Lei and Wang, Yan and Björn, Lars Olof and Li, Shaoshan},
  issn         = {0032-0935},
  keyword      = {Arabidopsis,CDK,CPD,Cyclin D,Cell cycle,G1-to-S transition,UV-B,Root tip},
  language     = {eng},
  number       = {4},
  pages        = {831--841},
  publisher    = {Springer},
  series       = {Planta},
  title        = {UV-B-induced DNA damage mediates expression changes of cell cycle regulatory genes in Arabidopsis root tips},
  url          = {http://dx.doi.org/10.1007/s00425-010-1340-5},
  volume       = {233},
  year         = {2011},
}