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Nucleotide-binding and oligomerization domain-like receptors and retinoic acid inducible gene-like receptors in human tonsillar T lymphocytes.

Petterson, Terese; Månsson, Anne; Riesbeck, Kristian LU and Cardell, Lars-Olaf LU (2011) In Immunology 133. p.84-93
Abstract
Nucleotide-binding and oligomerization domain (NOD) -like receptors (NLRs) and retinoic acid-inducible gene (RIG) -like receptors (RLRs) are recently discovered cytosolic pattern-recognition receptors sensing mainly bacterial components and viral RNA, respectively. Their importance in various cells and disorders is becoming better understood, but their role in human tonsil-derived T lymphocytes remains to be elucidated. In this study, we evaluated expression and functional relevance of NLRs and RLRs in human tonsillar CD3(+) T lymphocytes. Immunohistochemistry, real-time RT-PCR and flow cytometry revealed expression of NOD1, NOD2, NALP1, NALP3, NAIP, IPAF, RIG-1, MDA-5 and LGP-2 at mRNA and protein levels. Because of the limited number of... (More)
Nucleotide-binding and oligomerization domain (NOD) -like receptors (NLRs) and retinoic acid-inducible gene (RIG) -like receptors (RLRs) are recently discovered cytosolic pattern-recognition receptors sensing mainly bacterial components and viral RNA, respectively. Their importance in various cells and disorders is becoming better understood, but their role in human tonsil-derived T lymphocytes remains to be elucidated. In this study, we evaluated expression and functional relevance of NLRs and RLRs in human tonsillar CD3(+) T lymphocytes. Immunohistochemistry, real-time RT-PCR and flow cytometry revealed expression of NOD1, NOD2, NALP1, NALP3, NAIP, IPAF, RIG-1, MDA-5 and LGP-2 at mRNA and protein levels. Because of the limited number of ligands (iE-DAP, MDP, Alum, Poly(I:C)/LyoVec), functional evaluation was restricted to NOD1, NOD2, NALP3 and RIG-1/MDA-5, respectively. Stimulation with the agonists alone was not enough to induce activation but upon triggering via CD3 and CD28, a profound induction of proliferation was seen in purified CD3(+) T cells. However, the proliferative response was not further enhanced by the cognate ligands. Nonetheless, in tonsillar mononuclear cells iE-DAP, MDP and Poly(I:C)/LyoVec were found to augment the CD3/CD28-induced proliferation of tonsillar mononuclear cells. Also, iE-DAP and MDP were found to promote secretion of interleukins 2 and 10 as well as to up-regulate CD69. This study demonstrates for the first time a broad range of NLRs and RLRs in human tonsillar T cells and that NOD1, NOD2 and RIG-1/MDA-5 act synergistically with αCD3 and αCD28 to induce proliferation of human T cells. Hence, these results suggest that these receptors have a role in T-cell activation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Immunology
volume
133
pages
84 - 93
publisher
Wiley-Blackwell
external identifiers
  • wos:000289160000009
  • pmid:21342182
  • scopus:79953312835
ISSN
0019-2805
DOI
10.1111/j.1365-2567.2011.03414.x
language
English
LU publication?
yes
id
7d8a19b6-c6fa-4992-9a01-18eb70c138b2 (old id 1831594)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21342182?dopt=Abstract
date added to LUP
2011-03-01 17:39:15
date last changed
2017-10-22 04:54:28
@article{7d8a19b6-c6fa-4992-9a01-18eb70c138b2,
  abstract     = {Nucleotide-binding and oligomerization domain (NOD) -like receptors (NLRs) and retinoic acid-inducible gene (RIG) -like receptors (RLRs) are recently discovered cytosolic pattern-recognition receptors sensing mainly bacterial components and viral RNA, respectively. Their importance in various cells and disorders is becoming better understood, but their role in human tonsil-derived T lymphocytes remains to be elucidated. In this study, we evaluated expression and functional relevance of NLRs and RLRs in human tonsillar CD3(+) T lymphocytes. Immunohistochemistry, real-time RT-PCR and flow cytometry revealed expression of NOD1, NOD2, NALP1, NALP3, NAIP, IPAF, RIG-1, MDA-5 and LGP-2 at mRNA and protein levels. Because of the limited number of ligands (iE-DAP, MDP, Alum, Poly(I:C)/LyoVec), functional evaluation was restricted to NOD1, NOD2, NALP3 and RIG-1/MDA-5, respectively. Stimulation with the agonists alone was not enough to induce activation but upon triggering via CD3 and CD28, a profound induction of proliferation was seen in purified CD3(+) T cells. However, the proliferative response was not further enhanced by the cognate ligands. Nonetheless, in tonsillar mononuclear cells iE-DAP, MDP and Poly(I:C)/LyoVec were found to augment the CD3/CD28-induced proliferation of tonsillar mononuclear cells. Also, iE-DAP and MDP were found to promote secretion of interleukins 2 and 10 as well as to up-regulate CD69. This study demonstrates for the first time a broad range of NLRs and RLRs in human tonsillar T cells and that NOD1, NOD2 and RIG-1/MDA-5 act synergistically with αCD3 and αCD28 to induce proliferation of human T cells. Hence, these results suggest that these receptors have a role in T-cell activation.},
  author       = {Petterson, Terese and Månsson, Anne and Riesbeck, Kristian and Cardell, Lars-Olaf},
  issn         = {0019-2805},
  language     = {eng},
  pages        = {84--93},
  publisher    = {Wiley-Blackwell},
  series       = {Immunology},
  title        = {Nucleotide-binding and oligomerization domain-like receptors and retinoic acid inducible gene-like receptors in human tonsillar T lymphocytes.},
  url          = {http://dx.doi.org/10.1111/j.1365-2567.2011.03414.x},
  volume       = {133},
  year         = {2011},
}