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Matrilysin Expression Related to Radiation and Microflora Changes in Murine Bowel.

Polistena, Andrea LU ; Johnson, Louis Banka LU ; Röme, Andrada LU ; Wittgren, Lena LU ; Bäck, Sven LU ; Osman, Nadia LU ; Molin, Göran; Adawi, Diya LU and Jeppsson, Bengt LU (2011) In The Journal of surgical research 167. p.137-143
Abstract
BACKGROUND: Matrilysin (MMP-7) elevation after radiotherapy is shown in humans. Matrilysin regulates certain cytokines and the production of bactericidal proteins when the mucosa is exposed to bacterial antigens. We investigate the effect of irradiation on matrilysin and microflora in murine bowel, after modulation with antibiotics. METHODS: Animals were divided into two different groups a radiation group (72 animals) and sham radiation group (36 animals). Animals were divided into smaller groups of six according to radiation dose (19 or 38 Gy or sham). Seven days before radiotherapy ampicillin 500 mg/kg/d was administered intramuscularly, in the antibiotic groups. An exteriorized segment of ileum was subjected to single high dose... (More)
BACKGROUND: Matrilysin (MMP-7) elevation after radiotherapy is shown in humans. Matrilysin regulates certain cytokines and the production of bactericidal proteins when the mucosa is exposed to bacterial antigens. We investigate the effect of irradiation on matrilysin and microflora in murine bowel, after modulation with antibiotics. METHODS: Animals were divided into two different groups a radiation group (72 animals) and sham radiation group (36 animals). Animals were divided into smaller groups of six according to radiation dose (19 or 38 Gy or sham). Seven days before radiotherapy ampicillin 500 mg/kg/d was administered intramuscularly, in the antibiotic groups. An exteriorized segment of ileum was subjected to single high dose radiation (19 or 38 Gy). Samples were collected 2, 24, and 48 h and analyzed for microflora, MIP-2, TGF-β, and MMP-7. RESULTS: The combination of antibiotics and irradiation leads to an early significant reduction of bacteria, down-regulates MIP-2, up-regulates TGF-β and elevation of MMP-7 to levels achieved by antibiotics or irradiation alone. Lactobacilli were reduced to non-existent levels after antibiotics. CONCLUSIONS: Pretreatment with Ampicillin before irradiation and laparotomy in a murine model leads to Matrilysin over-expression as achieved by radiotherapy alone. Microfloral regulation does not affect MMP-7 stimulation after surgical or radiological trauma. Radiotherapy overrides the effect of antibiotics leading to an up-regulation of MMP-7, TGF-β and MIP-2 expression between 24 h and 48 h. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of surgical research
volume
167
pages
137 - 143
publisher
Elsevier
external identifiers
  • wos:000289407800011
  • pmid:21324398
  • scopus:79954616714
ISSN
1095-8673
DOI
10.1016/j.jss.2010.12.031
language
English
LU publication?
yes
id
2cb3993f-61be-4cfb-89b4-7f9b120a4e32 (old id 1831771)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21324398?dopt=Abstract
date added to LUP
2011-03-01 15:58:50
date last changed
2017-05-14 04:26:09
@article{2cb3993f-61be-4cfb-89b4-7f9b120a4e32,
  abstract     = {BACKGROUND: Matrilysin (MMP-7) elevation after radiotherapy is shown in humans. Matrilysin regulates certain cytokines and the production of bactericidal proteins when the mucosa is exposed to bacterial antigens. We investigate the effect of irradiation on matrilysin and microflora in murine bowel, after modulation with antibiotics. METHODS: Animals were divided into two different groups a radiation group (72 animals) and sham radiation group (36 animals). Animals were divided into smaller groups of six according to radiation dose (19 or 38 Gy or sham). Seven days before radiotherapy ampicillin 500 mg/kg/d was administered intramuscularly, in the antibiotic groups. An exteriorized segment of ileum was subjected to single high dose radiation (19 or 38 Gy). Samples were collected 2, 24, and 48 h and analyzed for microflora, MIP-2, TGF-β, and MMP-7. RESULTS: The combination of antibiotics and irradiation leads to an early significant reduction of bacteria, down-regulates MIP-2, up-regulates TGF-β and elevation of MMP-7 to levels achieved by antibiotics or irradiation alone. Lactobacilli were reduced to non-existent levels after antibiotics. CONCLUSIONS: Pretreatment with Ampicillin before irradiation and laparotomy in a murine model leads to Matrilysin over-expression as achieved by radiotherapy alone. Microfloral regulation does not affect MMP-7 stimulation after surgical or radiological trauma. Radiotherapy overrides the effect of antibiotics leading to an up-regulation of MMP-7, TGF-β and MIP-2 expression between 24 h and 48 h.},
  author       = {Polistena, Andrea and Johnson, Louis Banka and Röme, Andrada and Wittgren, Lena and Bäck, Sven and Osman, Nadia and Molin, Göran and Adawi, Diya and Jeppsson, Bengt},
  issn         = {1095-8673},
  language     = {eng},
  pages        = {137--143},
  publisher    = {Elsevier},
  series       = {The Journal of surgical research},
  title        = {Matrilysin Expression Related to Radiation and Microflora Changes in Murine Bowel.},
  url          = {http://dx.doi.org/10.1016/j.jss.2010.12.031},
  volume       = {167},
  year         = {2011},
}