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N-nitrosamines are associated with shorter telomere length.

Li, Huiqi LU ; Jönsson, Bo A LU ; Lindh, Christian LU ; Albin, Maria LU and Broberg Palmgren, Karin LU (2011) In Scandinavian Journal of Work, Environment and Health 37. p.316-324
Abstract
OBJECTIVES: Telomeres are critical to maintain the integrity of the chromosomes, and telomere abnormalities are important features of carcinogenesis. Telomere length differs among individuals due to genetic and environmental factors. Aiming to examine the relationship between DNA-damaging agents and average telomere length in peripheral blood, we conducted a cross-sectional study among 157 workers working in the rubber industry in Sweden. METHODS: N-nitrosamines were measured in air by personal sampling on Thermosorb/N tubes and analyzed by liquid chromatography tandem mass spectrometry (LC/MS/MS) for 60 individuals. Based on a similar working situation, the exposure was estimated for all workers. Polycyclic aromatic hydrocarbons (PAH)... (More)
OBJECTIVES: Telomeres are critical to maintain the integrity of the chromosomes, and telomere abnormalities are important features of carcinogenesis. Telomere length differs among individuals due to genetic and environmental factors. Aiming to examine the relationship between DNA-damaging agents and average telomere length in peripheral blood, we conducted a cross-sectional study among 157 workers working in the rubber industry in Sweden. METHODS: N-nitrosamines were measured in air by personal sampling on Thermosorb/N tubes and analyzed by liquid chromatography tandem mass spectrometry (LC/MS/MS) for 60 individuals. Based on a similar working situation, the exposure was estimated for all workers. Polycyclic aromatic hydrocarbons (PAH) were measured as the metabolite 1-hydroxypyrene (1-HP) in urine by LC. Carbon disulphide (CS2) was measured as the metabolite 2-thiothiazolidine-4-carboxylic acid (TTCA) in urine by LC/MS/MS. Toluidines (orto-, meta-, and para-) were measured in urine by gas chromatography (GC)/MS. The average telomere length in peripheral blood was determined by quantitative polymerase chain reaction (PCR). RESULTS: There was a reduction in telomere length with increasing exposure to N-nitrosamines in air [measured (N=60) N-nitrosamines β-coefficient= -10, (95% confidence interval [95% CI] -19- -1.2) P=0.026; estimated (N=157) N-nitrosamines β-coefficient = -5.3, (95% CI -9.5- -0.97) P=0.016]. Also, there were negative associations between para-toluidine [β-coefficient= -0.031 (95% CI -0.055- -0.0063) P=0.014], as well as age β-coefficient= -0.005 (95% CI -0.007- -0.002) P=0.001] and telomere length. There were no strong associations between other exposures and telomere length nor did smoking modify the effect. CONCLUSION: N-nitrosamines exposure may lead to telomere shortening. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Work, Environment and Health
volume
37
pages
316 - 324
publisher
Finnish Institute of Occupational Health
external identifiers
  • wos:000292362000007
  • pmid:21321788
  • scopus:79960036410
ISSN
0355-3140
DOI
10.5271/sjweh.3150
language
English
LU publication?
yes
id
051bf925-c2d3-4229-8bae-63aeed4bd6da (old id 1831833)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21321788?dopt=Abstract
date added to LUP
2011-03-01 15:03:50
date last changed
2017-11-12 04:06:36
@article{051bf925-c2d3-4229-8bae-63aeed4bd6da,
  abstract     = {OBJECTIVES: Telomeres are critical to maintain the integrity of the chromosomes, and telomere abnormalities are important features of carcinogenesis. Telomere length differs among individuals due to genetic and environmental factors. Aiming to examine the relationship between DNA-damaging agents and average telomere length in peripheral blood, we conducted a cross-sectional study among 157 workers working in the rubber industry in Sweden. METHODS: N-nitrosamines were measured in air by personal sampling on Thermosorb/N tubes and analyzed by liquid chromatography tandem mass spectrometry (LC/MS/MS) for 60 individuals. Based on a similar working situation, the exposure was estimated for all workers. Polycyclic aromatic hydrocarbons (PAH) were measured as the metabolite 1-hydroxypyrene (1-HP) in urine by LC. Carbon disulphide (CS2) was measured as the metabolite 2-thiothiazolidine-4-carboxylic acid (TTCA) in urine by LC/MS/MS. Toluidines (orto-, meta-, and para-) were measured in urine by gas chromatography (GC)/MS. The average telomere length in peripheral blood was determined by quantitative polymerase chain reaction (PCR). RESULTS: There was a reduction in telomere length with increasing exposure to N-nitrosamines in air [measured (N=60) N-nitrosamines β-coefficient= -10, (95% confidence interval [95% CI] -19- -1.2) P=0.026; estimated (N=157) N-nitrosamines β-coefficient = -5.3, (95% CI -9.5- -0.97) P=0.016]. Also, there were negative associations between para-toluidine [β-coefficient= -0.031 (95% CI -0.055- -0.0063) P=0.014], as well as age β-coefficient= -0.005 (95% CI -0.007- -0.002) P=0.001] and telomere length. There were no strong associations between other exposures and telomere length nor did smoking modify the effect. CONCLUSION: N-nitrosamines exposure may lead to telomere shortening.},
  author       = {Li, Huiqi and Jönsson, Bo A and Lindh, Christian and Albin, Maria and Broberg Palmgren, Karin},
  issn         = {0355-3140},
  language     = {eng},
  pages        = {316--324},
  publisher    = {Finnish Institute of Occupational Health},
  series       = {Scandinavian Journal of Work, Environment and Health},
  title        = {N-nitrosamines are associated with shorter telomere length.},
  url          = {http://dx.doi.org/10.5271/sjweh.3150},
  volume       = {37},
  year         = {2011},
}