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Isolation and characterization of progenitor-like cells from human renal proximal tubules.

Lindgren, David LU ; Boström, Anna-Karin LU ; Nilsson, Kristina LU ; Hansson, Jennifer LU ; Sjölund, Jonas LU ; Möller, Christina LU ; Jirström, Karin LU ; Nilsson, Elise LU ; Landberg, Göran LU and Axelson, Håkan LU , et al. (2011) In American Journal of Pathology 178(2). p.828-837
Abstract
The tubules of the kidney display a remarkable capacity for self-renewal on damage. Whether this regeneration is mediated by dedifferentiating surviving cells or, as recently suggested, by stem cells has not been unequivocally settled. Herein, we demonstrate that aldehyde dehydrogenase (ALDH) activity may be used for isolation of cells with progenitor characteristics from adult human renal cortical tissue. Gene expression profiling of the isolated ALDH(high) and ALDH(low) cell fractions followed by immunohistochemical interrogation of renal tissues enabled us to delineate a tentative progenitor cell population scattered through the proximal tubules (PTs). These cells expressed CD24 and CD133, previously described markers for renal... (More)
The tubules of the kidney display a remarkable capacity for self-renewal on damage. Whether this regeneration is mediated by dedifferentiating surviving cells or, as recently suggested, by stem cells has not been unequivocally settled. Herein, we demonstrate that aldehyde dehydrogenase (ALDH) activity may be used for isolation of cells with progenitor characteristics from adult human renal cortical tissue. Gene expression profiling of the isolated ALDH(high) and ALDH(low) cell fractions followed by immunohistochemical interrogation of renal tissues enabled us to delineate a tentative progenitor cell population scattered through the proximal tubules (PTs). These cells expressed CD24 and CD133, previously described markers for renal progenitors of Bowman's capsule. Furthermore, we show that the PT cells, and the glomerular progenitors, are positive for KRT7, KRT19, BCL2, and vimentin. In addition, tubular epithelium regenerating on acute tubular necrosis displayed long stretches of CD133(+)/VIM(+) cells, further substantiating that these cells may represent a progenitor cell population. Furthermore, a potential association of these progenitor cells with papillary renal cell carcinoma was discovered. Taken together, our data demonstrate the presence of a previously unappreciated subset of the PT cells that may be endowed with a more robust phenotype, allowing increased resistance to acute renal injury, enabling rapid repopulation of the tubules. (Less)
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American Journal of Pathology
volume
178
issue
2
pages
828 - 837
publisher
American Society for Investigative Pathology
external identifiers
  • wos:000287264400038
  • pmid:21281815
  • scopus:79951849286
ISSN
1525-2191
DOI
10.1016/j.ajpath.2010.10.026
language
English
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yes
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a3d70ec7-6d63-4ce4-b4c4-8f4c2b643cd8 (old id 1832439)
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http://www.ncbi.nlm.nih.gov/pubmed/21281815?dopt=Abstract
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2011-03-01 11:24:06
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2017-11-19 04:17:06
@article{a3d70ec7-6d63-4ce4-b4c4-8f4c2b643cd8,
  abstract     = {The tubules of the kidney display a remarkable capacity for self-renewal on damage. Whether this regeneration is mediated by dedifferentiating surviving cells or, as recently suggested, by stem cells has not been unequivocally settled. Herein, we demonstrate that aldehyde dehydrogenase (ALDH) activity may be used for isolation of cells with progenitor characteristics from adult human renal cortical tissue. Gene expression profiling of the isolated ALDH(high) and ALDH(low) cell fractions followed by immunohistochemical interrogation of renal tissues enabled us to delineate a tentative progenitor cell population scattered through the proximal tubules (PTs). These cells expressed CD24 and CD133, previously described markers for renal progenitors of Bowman's capsule. Furthermore, we show that the PT cells, and the glomerular progenitors, are positive for KRT7, KRT19, BCL2, and vimentin. In addition, tubular epithelium regenerating on acute tubular necrosis displayed long stretches of CD133(+)/VIM(+) cells, further substantiating that these cells may represent a progenitor cell population. Furthermore, a potential association of these progenitor cells with papillary renal cell carcinoma was discovered. Taken together, our data demonstrate the presence of a previously unappreciated subset of the PT cells that may be endowed with a more robust phenotype, allowing increased resistance to acute renal injury, enabling rapid repopulation of the tubules.},
  author       = {Lindgren, David and Boström, Anna-Karin and Nilsson, Kristina and Hansson, Jennifer and Sjölund, Jonas and Möller, Christina and Jirström, Karin and Nilsson, Elise and Landberg, Göran and Axelson, Håkan and Johansson, Martin},
  issn         = {1525-2191},
  language     = {eng},
  number       = {2},
  pages        = {828--837},
  publisher    = {American Society for Investigative Pathology},
  series       = {American Journal of Pathology},
  title        = {Isolation and characterization of progenitor-like cells from human renal proximal tubules.},
  url          = {http://dx.doi.org/10.1016/j.ajpath.2010.10.026},
  volume       = {178},
  year         = {2011},
}