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Increased GABA(A) channel subunits expression in CD8(+) but not in CD4(+) T cells in BB rats developing diabetes compared to their congenic littermates

Kumar Mendu, Suresh LU ; Åkesson, Lina LU ; Jin, Zhe LU ; Edlund, Anna LU ; Cilio, Corrado LU ; Lernmark, Åke LU and Birnira, Bryndis (2011) In Molecular Immunology 48(4). p.399-407
Abstract
GABA (gamma-aminobutyric acid), the main inhibitory neurotransmitter in the central nervous system is also present in the pancreatic islet beta cells where it may function as a paracrine molecule and perhaps as an immunomodulator of lymphocytes infiltrating the pancreatic islet. We examined CD4(+) and CD8(+) T cells from diabetes prone (DRlyp/lyp) or resistant (DR+/+) congenic biobreeding (BB) rats for expression of GABA(A) channels. Our results show that BB rat CD4(+) and CD8(+) T cells express alpha 1, alpha 2, alpha 3, alpha 4, alpha 6, beta 3, gamma 1, delta, rho 1 and rho 2 CABA(A) channel subunits. In CD8(+) T cells from DRlyp/lyp animals the subunits were significantly upregulated relative to expression levels in the CD8+ T cells... (More)
GABA (gamma-aminobutyric acid), the main inhibitory neurotransmitter in the central nervous system is also present in the pancreatic islet beta cells where it may function as a paracrine molecule and perhaps as an immunomodulator of lymphocytes infiltrating the pancreatic islet. We examined CD4(+) and CD8(+) T cells from diabetes prone (DRlyp/lyp) or resistant (DR+/+) congenic biobreeding (BB) rats for expression of GABA(A) channels. Our results show that BB rat CD4(+) and CD8(+) T cells express alpha 1, alpha 2, alpha 3, alpha 4, alpha 6, beta 3, gamma 1, delta, rho 1 and rho 2 CABA(A) channel subunits. In CD8(+) T cells from DRlyp/lyp animals the subunits were significantly upregulated relative to expression levels in the CD8+ T cells from DR+/+ rats as well as from CD4(+) T cells from both DRlyp/lyp and DR+/+ rats. Functional channels were formed in the T cells and physiological concentrations of GABA (100 nM) decreased T cell proliferation. Our results are consistent with the hypothesis that GABA in the islets of Langerhans may diminish inflammation by inhibition of activated T lymphocytes. (C) 2010 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
GABA, Diabetes, Lymphocytes, GABA(A) subunits, Proliferation, Immunomodulation
in
Molecular Immunology
volume
48
issue
4
pages
399 - 407
publisher
Pergamon
external identifiers
  • wos:000286955400004
  • scopus:78650599930
ISSN
1872-9142
DOI
10.1016/j.molimm.2010.08.005
language
English
LU publication?
yes
id
b3f4d52c-9e78-4dc8-85c5-33bfd077a765 (old id 1872617)
date added to LUP
2011-04-04 08:00:17
date last changed
2017-08-27 04:35:18
@article{b3f4d52c-9e78-4dc8-85c5-33bfd077a765,
  abstract     = {GABA (gamma-aminobutyric acid), the main inhibitory neurotransmitter in the central nervous system is also present in the pancreatic islet beta cells where it may function as a paracrine molecule and perhaps as an immunomodulator of lymphocytes infiltrating the pancreatic islet. We examined CD4(+) and CD8(+) T cells from diabetes prone (DRlyp/lyp) or resistant (DR+/+) congenic biobreeding (BB) rats for expression of GABA(A) channels. Our results show that BB rat CD4(+) and CD8(+) T cells express alpha 1, alpha 2, alpha 3, alpha 4, alpha 6, beta 3, gamma 1, delta, rho 1 and rho 2 CABA(A) channel subunits. In CD8(+) T cells from DRlyp/lyp animals the subunits were significantly upregulated relative to expression levels in the CD8+ T cells from DR+/+ rats as well as from CD4(+) T cells from both DRlyp/lyp and DR+/+ rats. Functional channels were formed in the T cells and physiological concentrations of GABA (100 nM) decreased T cell proliferation. Our results are consistent with the hypothesis that GABA in the islets of Langerhans may diminish inflammation by inhibition of activated T lymphocytes. (C) 2010 Elsevier Ltd. All rights reserved.},
  author       = {Kumar Mendu, Suresh and Åkesson, Lina and Jin, Zhe and Edlund, Anna and Cilio, Corrado and Lernmark, Åke and Birnira, Bryndis},
  issn         = {1872-9142},
  keyword      = {GABA,Diabetes,Lymphocytes,GABA(A) subunits,Proliferation,Immunomodulation},
  language     = {eng},
  number       = {4},
  pages        = {399--407},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {Increased GABA(A) channel subunits expression in CD8(+) but not in CD4(+) T cells in BB rats developing diabetes compared to their congenic littermates},
  url          = {http://dx.doi.org/10.1016/j.molimm.2010.08.005},
  volume       = {48},
  year         = {2011},
}