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Continuous full filling capillary electrochromatography-electrospraying chromatographic nanoparticles

Malmstrom, David; Axen, Jakob; Bergquist, Jonas; Viberg, Peter and Spégel, Peter LU (2011) In Electrophoresis 32(2). p.261-267
Abstract
The influence of instrumental parameters affecting the ionization in continuous full filling capillary electrochromatography/electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the BGE and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found to be optimal; increasing the pressure significantly decreased... (More)
The influence of instrumental parameters affecting the ionization in continuous full filling capillary electrochromatography/electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the BGE and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found to be optimal; increasing the pressure significantly decreased the separation efficiency due to the generation of a hydrodynamic flow. Generally, the ion strength of both the BGE and the sheath liquid were found to have very moderate effects on the separation of a homologous series of dialkyl phthalates, whereas the ionization efficiency was found to be unaffected by the nanoparticles and the separation efficiency was found to increase with increasing concentrations up to 3.8 mg/mL, whereafter it was observed to drop. The optimized method was linear over a wide concentration range and presented LOD and LOQ more than threefold lower than those previously reported using CFF-CEC/ESI-MS. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Continuous full filling capillary electrochromatography, Nanoparticles, Nebulizing gas pressure, Pseudostationary phase, Sheath liquid
in
Electrophoresis
volume
32
issue
2
pages
261 - 267
publisher
John Wiley & Sons
external identifiers
  • wos:000287156700009
  • scopus:78751628881
ISSN
0173-0835
DOI
10.1002/elps.201000261
language
English
LU publication?
yes
id
827dcb71-c725-4542-9a7e-4e2987ca3ca4 (old id 1878081)
date added to LUP
2011-04-27 09:40:03
date last changed
2017-10-22 03:12:08
@article{827dcb71-c725-4542-9a7e-4e2987ca3ca4,
  abstract     = {The influence of instrumental parameters affecting the ionization in continuous full filling capillary electrochromatography/electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the BGE and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found to be optimal; increasing the pressure significantly decreased the separation efficiency due to the generation of a hydrodynamic flow. Generally, the ion strength of both the BGE and the sheath liquid were found to have very moderate effects on the separation of a homologous series of dialkyl phthalates, whereas the ionization efficiency was found to be unaffected by the nanoparticles and the separation efficiency was found to increase with increasing concentrations up to 3.8 mg/mL, whereafter it was observed to drop. The optimized method was linear over a wide concentration range and presented LOD and LOQ more than threefold lower than those previously reported using CFF-CEC/ESI-MS.},
  author       = {Malmstrom, David and Axen, Jakob and Bergquist, Jonas and Viberg, Peter and Spégel, Peter},
  issn         = {0173-0835},
  keyword      = {Continuous full filling capillary electrochromatography,Nanoparticles,Nebulizing gas pressure,Pseudostationary phase,Sheath liquid},
  language     = {eng},
  number       = {2},
  pages        = {261--267},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Continuous full filling capillary electrochromatography-electrospraying chromatographic nanoparticles},
  url          = {http://dx.doi.org/10.1002/elps.201000261},
  volume       = {32},
  year         = {2011},
}