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Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma

Andersson, Cecilia LU ; Bergqvist, Anders LU ; Mori, Michiko LU ; Mauad, Thais; Bjermer, Leif LU and Erjefält, Jonas LU (2011) In Journal of Allergy and Clinical Immunology 127(4). p.123-905
Abstract
Background: A significant proportion of patients with asthma have persistent symptoms despite treatment with inhaled glucocorticosteroids. Objective: We hypothesized that in these patients, the alveolar parenchyma is subjected to mast cell-associated alterations. Methods: Bronchial and transbronchial biopsies from healthy controls (n = 8), patients with allergic rhinitis (n = 8), and patients with atopic uncontrolled asthma (symptoms despite treatment with inhaled glucocorticosteroids; mean dose, 743 mu g/d; n = 14) were processed for immunohistochemical identification of mast cell subtypes and mast cell expression of Fc epsilon RI and surface-bound IgE. Results: Whereas no difference in density of total bronchial mast cells was observed... (More)
Background: A significant proportion of patients with asthma have persistent symptoms despite treatment with inhaled glucocorticosteroids. Objective: We hypothesized that in these patients, the alveolar parenchyma is subjected to mast cell-associated alterations. Methods: Bronchial and transbronchial biopsies from healthy controls (n = 8), patients with allergic rhinitis (n = 8), and patients with atopic uncontrolled asthma (symptoms despite treatment with inhaled glucocorticosteroids; mean dose, 743 mu g/d; n = 14) were processed for immunohistochemical identification of mast cell subtypes and mast cell expression of Fc epsilon RI and surface-bound IgE. Results: Whereas no difference in density of total bronchial mast cells was observed between patients with asthma and healthy controls, the total alveolar mast cell density was increased in the patients with asthma (P < .01). Division into mast cell subtypes revealed that in bronchi of patients with asthma, tryptase positive mast cells (MCT) numbers decreased compared with controls (P <= .05), whereas tryptase and chymase positive mast cells (MCTC) increased (P <= .05). In the alveolar parenchyma from patients with asthma, an increased density was found for both MCT (P <= .05) and MCTC (P <= .05). The increased alveolar mast cell densities were paralleled by an increased mast cell expression of FceRI (P < .001) compared with the controls. The patients with asthma also had increased numbers (P < .001) and proportions (P < .001) of alveolar mast cells with surface-bound IgE. Similar increases in densities, FceRI expression, and surface-bound IgE were not seen in separate explorations of alveolar mast cells in patients with allergic rhinitis. Conclusion: Our data suggest that patients with atopic uncontrolled asthma have an increased parenchymal infiltration of MCT and MCTC populations with increased expression of FceRI and surface-bound IgE compared with atopic and nonatopic controls. (J Allergy Clin Immunol 2011;127:905-12.) (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Mast cells, asthma, Fc epsilon RI, IgE, allergy, peripheral, inflammation, alveolar parenchyma
in
Journal of Allergy and Clinical Immunology
volume
127
issue
4
pages
123 - 905
publisher
Elsevier
external identifiers
  • wos:000289055800011
  • scopus:79953670244
ISSN
1097-6825
DOI
10.1016/j.jaci.2011.01.022
language
English
LU publication?
yes
id
1afeb6c7-6344-4a01-92d3-1b62e8b22490 (old id 1918448)
date added to LUP
2011-05-02 11:13:50
date last changed
2017-08-27 04:56:56
@article{1afeb6c7-6344-4a01-92d3-1b62e8b22490,
  abstract     = {Background: A significant proportion of patients with asthma have persistent symptoms despite treatment with inhaled glucocorticosteroids. Objective: We hypothesized that in these patients, the alveolar parenchyma is subjected to mast cell-associated alterations. Methods: Bronchial and transbronchial biopsies from healthy controls (n = 8), patients with allergic rhinitis (n = 8), and patients with atopic uncontrolled asthma (symptoms despite treatment with inhaled glucocorticosteroids; mean dose, 743 mu g/d; n = 14) were processed for immunohistochemical identification of mast cell subtypes and mast cell expression of Fc epsilon RI and surface-bound IgE. Results: Whereas no difference in density of total bronchial mast cells was observed between patients with asthma and healthy controls, the total alveolar mast cell density was increased in the patients with asthma (P &lt; .01). Division into mast cell subtypes revealed that in bronchi of patients with asthma, tryptase positive mast cells (MCT) numbers decreased compared with controls (P &lt;= .05), whereas tryptase and chymase positive mast cells (MCTC) increased (P &lt;= .05). In the alveolar parenchyma from patients with asthma, an increased density was found for both MCT (P &lt;= .05) and MCTC (P &lt;= .05). The increased alveolar mast cell densities were paralleled by an increased mast cell expression of FceRI (P &lt; .001) compared with the controls. The patients with asthma also had increased numbers (P &lt; .001) and proportions (P &lt; .001) of alveolar mast cells with surface-bound IgE. Similar increases in densities, FceRI expression, and surface-bound IgE were not seen in separate explorations of alveolar mast cells in patients with allergic rhinitis. Conclusion: Our data suggest that patients with atopic uncontrolled asthma have an increased parenchymal infiltration of MCT and MCTC populations with increased expression of FceRI and surface-bound IgE compared with atopic and nonatopic controls. (J Allergy Clin Immunol 2011;127:905-12.)},
  author       = {Andersson, Cecilia and Bergqvist, Anders and Mori, Michiko and Mauad, Thais and Bjermer, Leif and Erjefält, Jonas},
  issn         = {1097-6825},
  keyword      = {Mast cells,asthma,Fc epsilon RI,IgE,allergy,peripheral,inflammation,alveolar parenchyma},
  language     = {eng},
  number       = {4},
  pages        = {123--905},
  publisher    = {Elsevier},
  series       = {Journal of Allergy and Clinical Immunology},
  title        = {Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma},
  url          = {http://dx.doi.org/10.1016/j.jaci.2011.01.022},
  volume       = {127},
  year         = {2011},
}