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Immunoglobulin subclass profiles of anti-idiotypic antibodies to GAD65Ab differ between type 1 diabetes patients and healthy individuals.

Oak, Shilpa; Radtke, Jared; Törn, Carina LU ; Landin-Olsson, Mona LU and Hampe, Christiane S (2011) In Scandinavian Journal of Immunology 74. p.363-367
Abstract
Previously we reported the presence of anti-idiotypic antibodies (anti-Id) specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti-Id directed to GAD65Ab in T1D patients was significantly lower. These anti-Id recognize the antigen binding site of GAD65Ab, thus preventing their binding to GAD65. Here we characterized the IgG subclass profile of these anti-Id (GAD65Ab-specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti-Id in healthy individuals (n=16) was IgG3-dominated, while in T1D patients (n=8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti-Id in both healthy individuals (n=38) and GAD65Ab-negative T1D patients (n=35) showed a predominant rank... (More)
Previously we reported the presence of anti-idiotypic antibodies (anti-Id) specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti-Id directed to GAD65Ab in T1D patients was significantly lower. These anti-Id recognize the antigen binding site of GAD65Ab, thus preventing their binding to GAD65. Here we characterized the IgG subclass profile of these anti-Id (GAD65Ab-specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti-Id in healthy individuals (n=16) was IgG3-dominated, while in T1D patients (n=8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti-Id in both healthy individuals (n=38) and GAD65Ab-negative T1D patients (n=35) showed a predominant rank order of IgG1>IgG2>IgG4>IgG3. However, the frequency of GAD65Ab of the IgG4 subclass was significantly higher in T1D patients (p<0.05). We conclude that the IgG subclass profile of anti-Id (GAD65Ab-specific) in healthy individuals differs from that in T1D patients. These differences may provide insights in the development of these antibodies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Immunology
volume
74
pages
363 - 367
publisher
Wiley-Blackwell
external identifiers
  • WOS:000295075700004
  • PMID:21517929
  • Scopus:80052666659
ISSN
1365-3083
DOI
10.1111/j.1365-3083.2011.02565.x
language
English
LU publication?
yes
id
98740472-8aa1-4943-aa3d-e2da934c0a93 (old id 1936740)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21517929?dopt=Abstract
date added to LUP
2011-05-02 16:20:59
date last changed
2017-01-01 07:52:51
@article{98740472-8aa1-4943-aa3d-e2da934c0a93,
  abstract     = {Previously we reported the presence of anti-idiotypic antibodies (anti-Id) specific to autoantibodies against GAD65 (GAD65Ab) in healthy individuals while the activity of anti-Id directed to GAD65Ab in T1D patients was significantly lower. These anti-Id recognize the antigen binding site of GAD65Ab, thus preventing their binding to GAD65. Here we characterized the IgG subclass profile of these anti-Id (GAD65Ab-specific) and of the associated GAD65Ab themselves. The IgG subclass response of anti-Id in healthy individuals (n=16) was IgG3-dominated, while in T1D patients (n=8) IgG1 was the major IgG subclass. The GAD65Ab bound by anti-Id in both healthy individuals (n=38) and GAD65Ab-negative T1D patients (n=35) showed a predominant rank order of IgG1&gt;IgG2&gt;IgG4&gt;IgG3. However, the frequency of GAD65Ab of the IgG4 subclass was significantly higher in T1D patients (p&lt;0.05). We conclude that the IgG subclass profile of anti-Id (GAD65Ab-specific) in healthy individuals differs from that in T1D patients. These differences may provide insights in the development of these antibodies.},
  author       = {Oak, Shilpa and Radtke, Jared and Törn, Carina and Landin-Olsson, Mona and Hampe, Christiane S},
  issn         = {1365-3083},
  language     = {eng},
  pages        = {363--367},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {Immunoglobulin subclass profiles of anti-idiotypic antibodies to GAD65Ab differ between type 1 diabetes patients and healthy individuals.},
  url          = {http://dx.doi.org/10.1111/j.1365-3083.2011.02565.x},
  volume       = {74},
  year         = {2011},
}