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Analysis of chlormequat in human urine as a biomarker of exposure using liquid chromatography triple quadrupole mass spectrometry.

Lindh, Christian LU ; Littorin, Margareta LU ; Johannesson, Gunvor and Jönsson, Bo A LU (2011) In Journal of Chromatography. B 879(19). p.1551-1556
Abstract
In this study, a method using liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is described for the analysis of the plant growth regulator chlormequat (CCC) in human urine. Analysis was carried out using selected reaction monitoring (SRM) in the positive ion mode. [(2)H(4)] labeled CCC as internal standard (IS) was used for quantification of CCC. The limit of detection (LOD) was determined to 0.1ng/mL. The method was linear in the range 0.3-800ng/mL urine and had a within-run precision of 4-9%. The between-run precision was determined at urine levels of 7.0 and 31ng/mL and found to be 5 and 6% respectively. The reproducibility was 3-6%. To validate CCC as a biomarker of exposure, the method was applied in a human... (More)
In this study, a method using liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is described for the analysis of the plant growth regulator chlormequat (CCC) in human urine. Analysis was carried out using selected reaction monitoring (SRM) in the positive ion mode. [(2)H(4)] labeled CCC as internal standard (IS) was used for quantification of CCC. The limit of detection (LOD) was determined to 0.1ng/mL. The method was linear in the range 0.3-800ng/mL urine and had a within-run precision of 4-9%. The between-run precision was determined at urine levels of 7.0 and 31ng/mL and found to be 5 and 6% respectively. The reproducibility was 3-6%. To validate CCC as a biomarker of exposure, the method was applied in a human experimental oral exposure to CCC. Two healthy volunteers received 25μg/kg b.w. CCC in a single oral dose followed by urine sampling for 46h post-exposure. The CCC was estimated to follow a first order kinetic and a two compartment model with an elimination half-life of 2-3h and 10-14h respectively. One hundred 24h urine samples were collected from non-occupationally exposed individuals in the general population in southern Sweden. All samples had detectable levels above the LOD 0.1ng/mL urine. The median levels were 4ng/mL of CCC in unadjusted urine. The levels found in the population samples are several magnitudes lower than those found in the experimental exposure, which corresponds to an oral exposure of 50% of the ADI for CCC. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography. B
volume
879
issue
19
pages
1551 - 1556
publisher
Elsevier
external identifiers
  • wos:000291330600009
  • pmid:21497564
  • scopus:79955896812
ISSN
1873-376X
DOI
10.1016/j.jchromb.2011.03.046
language
English
LU publication?
yes
id
26b1e8c3-4baf-4d65-9260-a29ffedc62db (old id 1937050)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21497564?dopt=Abstract
date added to LUP
2011-05-02 14:43:14
date last changed
2017-08-06 03:03:26
@article{26b1e8c3-4baf-4d65-9260-a29ffedc62db,
  abstract     = {In this study, a method using liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is described for the analysis of the plant growth regulator chlormequat (CCC) in human urine. Analysis was carried out using selected reaction monitoring (SRM) in the positive ion mode. [(2)H(4)] labeled CCC as internal standard (IS) was used for quantification of CCC. The limit of detection (LOD) was determined to 0.1ng/mL. The method was linear in the range 0.3-800ng/mL urine and had a within-run precision of 4-9%. The between-run precision was determined at urine levels of 7.0 and 31ng/mL and found to be 5 and 6% respectively. The reproducibility was 3-6%. To validate CCC as a biomarker of exposure, the method was applied in a human experimental oral exposure to CCC. Two healthy volunteers received 25μg/kg b.w. CCC in a single oral dose followed by urine sampling for 46h post-exposure. The CCC was estimated to follow a first order kinetic and a two compartment model with an elimination half-life of 2-3h and 10-14h respectively. One hundred 24h urine samples were collected from non-occupationally exposed individuals in the general population in southern Sweden. All samples had detectable levels above the LOD 0.1ng/mL urine. The median levels were 4ng/mL of CCC in unadjusted urine. The levels found in the population samples are several magnitudes lower than those found in the experimental exposure, which corresponds to an oral exposure of 50% of the ADI for CCC.},
  author       = {Lindh, Christian and Littorin, Margareta and Johannesson, Gunvor and Jönsson, Bo A},
  issn         = {1873-376X},
  language     = {eng},
  number       = {19},
  pages        = {1551--1556},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Analysis of chlormequat in human urine as a biomarker of exposure using liquid chromatography triple quadrupole mass spectrometry.},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2011.03.046},
  volume       = {879},
  year         = {2011},
}